Pakistan Journal of Nutrition1680-51941994-7984Asian Network for Scientific Information10.3923/pjn.2018.446.453SugiyantiDina DarmadjiPurnama AnggrahiniSri AnwarChairil SantosoUmar 92018179Background and Objective: Shrimp shell waste and crab shell waste are causes of environmental pollution and chemical methods can be used to process this waste into chitosan. Chitosan is the second largest natural biopolymer after cellulose and has many applications in food and health products. The aim of this research was to investigate the potential of Tambak Lorok shrimp shell and crab shell waste as an alternative raw material for chitosan production. Materials and Methods: Chitosan was prepared by chemically treating shell waste from shrimp in the Penaeidae rafinesque family and shell waste from crab in the Portunidae family from Tambak Lorok, Indonesia. The chemical structure of chitosan was confirmed by infrared spectroscopy, oswald viscometry and scanning electron microscopy. Results: The isolated chitosan from shrimp shell waste showed an 89.6% degree of deacetylation, a 64% degree of crystallinity and a 557 kDa molecular weight. The isolated chitosan from crab shell waste showed an 82.1% degree of deacetylation, an 81% degree of crystallinity and a 690 kDa molecular weight. Chitosan from both shrimp shell waste and crab shell waste had a porous and fibril-like structure. Conclusion: It was concluded that shrimp shell waste and crab shell waste are potential alternative raw materials for chitosan production.]]>Shahidi, F., J.K.V. Arachchi and Y.J. Jeon,1999103751Kumar, M.N.V.R.,200046127Muzzarelli, R.A.A., 1977Dutta, P.K., S. Tripathi, G.K. Mehrotra and J. Dutta,200911411731182Dai, X., P. Chang, Q. Zhu, W. Liu, Y. Sun, S. Zhu and Z. Jiang,20135546469Sayari, N., A. Sila, B.E. Abdelmalek, R.B. Abdallah, S. Ellouz-Chaabouni, A. Bougatef and R. Balti,201687163171Van der Gronde, T., A. Hartog, C. van Hees, H. Pellikaan and T. Pieters,2016199746759Huang, C., K.G. Neoh, L. Xu, E.T. Kang and E. Chiong,20121325132520Jia, S., Z. Lu, Z. Gao, J. An and X. Wu et al.,20161-42-induced rat model of Alzheimer's disease.]]>83416425Khdair, A., I. Hamad, H. Alkhatib, Y. Bustanji, M. Mohammad, R. Tayem and K. Aiedeh,2016933844Li, T.S.C., T. Yawata and K. Honke,2014524861Abdelmalek, B.E., A. Sila, A, Haddar, A. Bougatef and M.A. Ayadi,2017104953962Darmadji, P. and M. Izumimoto,199438243254Devlieghere, F., A. Vermeulen and J. Debevere,200421703714Lasabuda, R.,2013192101Brine, C.J. and P.R. Austin,198169283286El Knidri, H., R. El Khalfaouy, A. Laajeb, A. Addaou and A. Lahsini,2016104395405AOAC.,2005Sabnis, S. and L.H. Block,1997396771Benhabiles, M.S., R. Salah, H. Lounici, N. Drouiche, M.F.A. Goosen and N. Mameri,2012294856Hajji, S., I. Younes, O. Ghorbel-Bellaaj, R. Hajji, M. Rinaudo, M. Nasri and K. Jellouli,201465298306Di Mario, F., P. Rapana, U. Tomati and E. Galli,200843812Park, S.I., K.S. Marsh and P. Dawson,201085493499Seng, J.M.,1988714044Kumari, S., S.H.K. Annamareddy, S. Abanti and P.K. Rath,201710416971705No, H.K. and S.P. Meyers,199542752Ma, B., A. Qin, X. Li, X. Zhao and C. He,201464341346Kaya, M., O. Seyyar, T. Baran and T. Turkes, 20142014Paulino, A.T., J.I. Simionato, J.C. Garcia and J. Nozaki,20066498103