Pakistan Journal of Nutrition1680-51941994-7984Asian Network for Scientific Information10.3923/pjn.2018.131.139Lactobacillus plantarum as Probiotic on In vitro Ruminal Fermentation and Microbial Population]]>D. AstutiWulansih G. WiryawanKomang WinaElizabeth WidyastutiYantyati SuhartiSri RidwanRoni 32018173Background and Objective: Probiotics are widely used in ruminant production, but information about the potential of Lactobacillus plantarum (L. plantarum) as a probiotic for ruminants is still limited. The aim of this research was to select L. plantarum strains as a probiotic for ruminants and to determine their effect on the rumen fermentation system. Materials and Methods: The first experiment was conducted using a randomized block design to select 14 strains of L. plantarum isolated from rumen cattle. The second experiment was arranged in a completely randomized design using two selected L. plantarum strains to determine their effects as a probiotic on rumen fermentation. The substrates used for in vitro fermentation were napier grass (Pennisetum purpureum) and concentrate in a 70:30 ratio. Results: From experiment 1, L. plantarum U32 was selected, because it produced low methane/total gas (27.39%) and strain U40 was selected because it had the highest dry matter and organic matter rumen disappearance (56.45 and 56.44%). In experiment 2, the addition of L. plantarum U32 and U40 as probiotics increased propionic acid and decreased acetic production (p<0.05), which led to a lower A:P ratio (p<0.05). The total volatile fatty acid and in vitro digestibility were not affected by the addition of L. plantarum. Probiotic addition increased lactic acid bacteria and the protozoa population (p<0.05) from the rumen fluid compared to the control. The total rumen bacteria were not significantly changed by the treatments. Conclusion: The addition of L. plantarum strains U32 and U40 as probiotics had beneficial effects for rumen fermentation due to increased propionic acid and decreased methane production.]]>Fuller, R.,198966365378FAO. and WHO.,20012001Jiao, P.X., L.Y. Wei, N.D. Walker, F.Z. Liu, L.Y. Chen, K.A. Beauchemin and W.Z. Yang,20172281322Antonius, K.G. Wiryawan, A. Thalib and A. Jayanegara,2015in vitro study.]]>143743Tannock, G.W.,200173410414Elghandour, M.M.Y., A.Z.M. Salem, J.S.M. Castaneda, L.M. Camacho, A.E. Kholif and J.C.V. Chagoya,201514526533Wiryawan, K.G. and J.D. Brooker,19954615551568Seo, J.K., S.W. Kim, M.H. Kim, S.D. Upadhaya, D.K. Kam and J.K. Ha,20102316571667Bureenok, S., W. Suksombat and Y. Kawamoto,2011Brachiaria ruziziensis) silages.]]>138266271Cobos, M.A., A.L. de Coss, N.D. Ramirez, S.S Gonzales and R.F. 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