Pakistan Journal of Nutrition1680-51941994-7984Asian Network for Scientific Information10.3923/pjn.2017.666.671AminahSiti Suparmo NarukiSri WuryastutyHastari 92017169Background and Objective: Soybeans are known for their positive influence on the prevention of osteoporosis in postmenopausal women. Soybean sprouts produce bioactive compounds that are better than soybeans. This study aimed to compare the potential of soybean sprouts with soybean and ethinylestradiol on the changes of biomarkers of bone turnover activity in ovariectomized rats (OVX). Materials and Methods: Twenty-five female Sprague-Dawley rats aged 2 months were placed into 5 groups: (i) normal control (without OVX) (N-C); (ii) OVX control (OVX-C); (iii) OVX+ethinylestradiol (30μg kg1 b.wt.,/day, orally) (OVX-E); (iv) OVX+soybean flour (based on a dose 10μg g1 b.wt.,/day of isoflavones, orally) (OVX-S); (v) OVX+soybean sprout flour (based on a dose 10μg g1 b.wt.,/day of isoflavones, orally) (OVX-SS). All groups were treated for 6 weeks and all rats were fed an AIN-93M-based diet. Blood samples were collected before and after treatment for analysis of serum biomarkers of bone turnover and estradiol hormone. Data were analyzed using one-way ANOVA, followed by Duncans Multiple-Range (DMR) test. Results: The increase of osteocalcin (OC) and beta-crosslaps (βCTx) in the serum of the OVX-SS group was lower than in the OVX-S group and was the same as the OVX-E group. The OVX-C group experienced the highest increase in OC and βCTx. All groups of OVX rats also experienced a significant decline in estradiol hormone. There was no difference in the decrease in serum estradiol in the OVX-S and OVX-SS groups. Conclusion: The results of the study show that soybean sprout flour consumption provides better inhibition of bone turnover activity than soybeans in ovariectomized rats. The potential of soy and soybean sprouts in estradiol hormone recovery on ovariectomized rats is not different.]]>NOF.,20132013Dhanwal, D.K., C. Cooper and E.M. Dennison,20102010Micosch, P.,20122012pp: 137-162pp: 137-162Nilsson, S. and J.A. Gustafsson,200237128Biver, E., F. Chopin, G. Coiffier, T.F. Brentano, B. Bouvard, P. Garnero and B. Cortet,2012792025King, J., C.H. Wynne, L. Assersohn and A. Jones,20114716231632Cui, Y., S. Deming-Halverson, A. Beeghly-Fadiel, L. Lipworth and M.J. Shrubsole et al.,20142011691178Borrelli, F. and E. Ernst,201066333343Wang, Q., X. Ge, X. Tian, Y. Zhang, J. Zhang and P. Zhang,20131697701Wafay, H.A., M. Abdel-Moniem, H.A. Megahed and H. Elmalt,20135152156Park, Y., H.J. Moon, D.J. Paik and D.Y. Kim,20137185191Alekel, D.L., M.D. van Loan, K.J. Koehler, L.N. Hanson and J.W. Stewart et al.,201091218230Oseni, O.A., A.U. Ibeto and M.O. Aruna,2011in-vitro multi enzyme digestibility of the seed of Brazilian Jack beans (Canavalia braziliensis).]]>2192197Afiukwa, C.A., O. Onwuchekwa, U.A. Ibiam, C.O. Edeogu and P.M. Aja,201262534Mugendi, J.B., E.N.M. Njag, E.N. Kuria, M.A. Mwasaru, J.G. Mureithi and Z. Apostolides,2010Mucuna pruriens L.).]]>4156166Li, L., B. Liu and X. Zheng,2011558945898Rusydi, M.R.M. and A. Azrina,201219673677Hartiningsih, D. Anggraini and D. Aji,201269297Reeves, P.G., F.H. Nielsen and G.C. Fahey Jr.,199312319391951Lelovas, P.P., T.T. Xanthos, S.E. Thoma, G.P. Lyritis and I.A. Dontas,200858424430Hoegh-Andersen, P., L.B. Tanko, T.L. Andersen, C.V. Lundberg and J.A. Mo et al.,20042004Lim, D.W. and Y.T. Kim,2014Angelica sinensis (Oliv.) Diels extract on ovariectomized rats and its oral toxicity in rats.]]>643624372Hertrampf, T., B. Schleipen, C. Offermanns, M. Velders, U. Laudenbach and P. Diel,2009184198203Lim, D.W., J.G. Kim and Y.T. Kim,2013Puerariae radix on lipid and bone metabolism in ovariectomized rats.]]>527342746Miao, Q., J.G. Li, S. Miao, N. Hu and J. Zhang et al.,2011135670Lerner, U.H.,200685584595Lee, Y.S., X. Chen and J.J.B. Anderson,20012112871298Chen, X.W., S.C. Garner and J.J.B. Anderson,2002295417422Chiang, S.S. and T.M. Pan,20139714891500Byun, J.S. and S.S. Lee,201056106112Picherit, C., C. Bennetau-Pelissero, B. Chanteranne, P. Lebecque, M.J. Davicco, J.P. Barlet and V. Coxam,2001131723728Jeon, B.J., J. Ahn and H.S. Kwak,20091212601267Muthusami, S., I. Ramachandra, B. Muthusamy, G. Vasudevan and V. Prabhu et al.,20053608186Arslan, A., S. Orkun, G. Aydin, I. Keles, A. Tosun, M. Arslan and O. Caglayan,20112011Norazlina, M., H. Hermizi, O. Faizah, A.N. Shuid, M. Norliza and S. Ima-Nirwana,20106505512Setiawan, D.I., K. Tjahyono and D.N. Afifah,2016132026Price, S.A. and L.M. Wilson,19944th Edn., Chapter 64,pp: 1125-1157pp: 1125-1157Hong, G.E., P.K. Mandal, C.W. Pyun, K. Choi and S.K. Kim et al.,20094288296Modder, U.I., B.L. Riggs, T.C. Spelsberg, D.G. Fraser, E.J. Atkinson, R. Arnold and S. Khosla,2004151503510