Pakistan Journal of Nutrition1680-51941994-7984Asian Network for Scientific Information10.3923/pjn.2017.148.154Oreochromis niloticus Fed Diets
with Different Protein Levels]]>RagaaNaela M. ElalaNermeen M. Abu KamalAzza M. KamelN.F. 32017163Objective: This study was conducted to investigate the effect of a serine-protease supplementation on growth performance and
protein digestibility of cultured Oreochromis niloticus fed diets with different crude protein (CP) levels. Materials and Methods: The diets
T1and T2 were formulated to supply 28 and 26% crude protein, respectively. A serine-protease enzyme (Ronozyme ProAct) product with
an activity of 75000 Protease (PROT)/g was added to the diets at the level of 0, 200 and 400 mg kg1 diet. In this study 384 fish with an
initial body weight of average 36.34±3.68 g were divided into 6 groups with 4 replicates of 16 fish/replicate. The experimental period
extended for 56 days. Results: The weight gain (WG), specific growth ratio (SGR) and protein efficiency ratio (PER), apparent protein
digestibility (APD) were significantly increased when T1 and T2 diets were supplemented with protease enzyme while feed conversion
ratio (FCR) was significantly decreased. Fish fed T2 diet supplemented with 400 mg kg1 protease showed a non-significant difference
in final weight, SGR, PER and APD comparing with fish fed T1 diet only. Conclusion: The results indicated that the protease enzyme
supplementation can significantly improve growth performance and protein digestibility in O. niloticus . Protease enzyme could be used
to reduce the protein content of the diet with maintaining the fish performance.]]>FAO.,2014Pages: 223Pages: 223FAO.,20112011Wang, M. and M. Lu,20164723632374Hardy, R.W.,19951995pp: 26-50pp: 26-50Kumar, S., N.P. Sahu, A.K. Pal, D. Choudhury, S. Yengkokpam and S.C. Mukherjee,2005L. rohita juveniles.]]>19331344Khattab, R.Y. and S.D. Arntfield,20094211131118Adeola, O. and A.J. Cowieson,20118931893218Dias, J., P. Rema, C. Nunes, K. Vogel and V. Verlhac,20122012Cowieson, A.J. and O. Adeola,20058418601867Cowieson, A.J. and V. Ravindran,200849340346Simbaya, J., B.A. Silominski, W. Guenter, A. Morgan and L.D. Campbell, 1996in vitro and in vivo studies.]]>61219234Ghazi, S., J.A. Rooke and H. Galbraith,200344410418Kamel, N.F., Naela, M. Ragaa, R.A. El-Banna and F.F. Mohamed,20156216225NRC.,1993Pages: 128Pages: 128El-Ashram, A.M.M. and M.E. El-Boshy,2008Oreochromis niloticus).]]>2008pp: 10791107AOAC.,199516th Edn.,Pages: 1298Pages: 1298Abu-Elala, N., M. Marzouk and M. Moustafa,2013Saccharomyces cerevisiae biotic forms as immune-modulator and growth promoter for Oreochromis niloticus challenged with some fish pathogens.]]>12129Bureau, D.P., A.M. Harris and C.Y. Cho,1999Oncorhynchus mykiss) diets.]]>180345358Zhou, Q.C., B.P. Tan, K.S. Mai and Y.J. Liu,2004Rachycentron canadum.]]>241441451Farukawa, A. and H. Tsukahara,196632502506Daniel, W.W. and C.L. Cross,201310th Edn.,Pages: 960Pages: 960Moyle, P.B. and J.J. Cech,20004th Edn.,Pages: 612Pages: 612De Silva, S.S., R.M. Gunasekera and K.F. Shim,199195305318Ayodeji, A.A., D.L. Merrifield and S.D. Davies,2014Oreochromis niloticus.]]>2014pp: 92Soares, E.C., M.P. Filho, R. Roubach and R.C. Soares e Silva,2008Cichla sp.) juvenile].]]>37971976, (In Portuguese)Drew, M.D., V.J. Racz, R. Gauthier and D.L. Thiessen,2005Oncorhynchus mykiss).]]>119117128Adeoye, A.A., A. Jaramillo-Torres, S.W. Fox, D.L. Merrifield and S.J. Davies,2016Oreochromis niloticus) with selected exogenous enzymes: Overall performance and effects on intestinal histology and microbiota.]]>215133143De Silva, S.S. and T.A. Anderson,1995Pages: 319Pages: 319Hara, S., H. Kohno and Y. Taki,1986Siganus guttatus, in the laboratory.]]>59273285Chen, J.M., J.Y. Ye, Y.X. Xu, B.Q. Shen, J.L. Guo, Q. Pan and Y.H. Wang,2009Mylopharyngodon piceus).]]>33726731Bedford, M.R. and J. Inborr,19931993pp: 485489Lin, S., K. Mai and B. Tan,2007Oreochromis niloticus × O. aureus.]]>3816451653