Pakistan Journal of Nutrition1680-51941994-7984Asian Network for Scientific Information10.3923/pjn.2010.307.313Ardisia compressa]]>Ramirez-MaresMarco Vinicio Sanchez-BurgosJorge Alberto Hernandez-CarlosBeatriz 4201094The in vitro antioxidant, antimicrobial and antitopoisomerase activities of crude Methanolic (Me) and Hexane (He) extracts of the stem bark of Ardisia compressa (AC) were investigated. Free radical-scavenging activity against 2,2-Diphenyl-1-picrylhydrazyl (DPPH) was evaluated, acid ascorbic was used as reference standard. Antimicrobial activity was determined using the agar diffusion method; the bacteria K. pneumoniae, E. coli, S. epidermidis and the fungus C. albicans. Controls employed were chloranphenicol (30 μg) and nystatin (100 units). Topoisomerase inhibition was determined by a clone-forming assay, which uses yeast (S. cerevisiae) strains as a model. Controls included dimethyl sulfoxide (1.66%); campothecin (50 μg/ml), etoposide (100 μg/ml) and sobuzoxane (150 μg/ml). Ascorbic acid was a substantially more powerful antioxidant than the extracts from the stem bark of AC. The Antiradical Efficiency (AE) of ascorbic acid was 249-fold that for He and 8.3-fold that for Me. Only the growth of Klebsiella pneumoniae was inhibited by Me (MIC = 1.875 mg/ml) and He (MIC = 0.9375 mg/ml), while the growth of the other strains were not inhibited. He extract not showed antitopoisomerase activity. Me extract showed antitopoisomerase activity (-15.3%). Open column chromatography of the methanolic extract was conducted and fourteen fractions were collected and tested. Fractions VI (-21.55%), X (-42.49%), XI (-97.68%), XII (-52.34%) and XIII (-35.97%) showed antitopoisomerase I activity and fraction XIV (-79%) antitopoisomerase II (poison) activity. These results suggest that Me extract of the stem bark of A. compressa could be promising in its potential usefulness for treatment of cancer and deserves further investigation.]]>Adhami, V.M. and H. Mukhtar,2007375257Brand-Williams, W., M.E. Cuvelier and C. Berset,1995282530Burgess, J.G., E.M. Jordan, M. Bregu, A. Mearns-Spragg and K.G. Boyd,1999702732Chitra, M., E. Sukumar, V. Suja and C.S. Devi,199440109113Cho, K.H., J.M. Pezzuto, J.L. Bolton, V.E. Steele, G.J. Kelloff, S.K. Lee and A. Constantinou,20003621462156Gonzalez de Mejia, E.G., M.V. Ramirez-Mares and M.G. Nair,2002Ardisia compressa and ardisin protect against benomyl oxidation of hepatocytes.]]>5077147719De Mejia, E.G., M.V. Ramirez-Mares, E. Arce-Popoca, M. Wallig and S. Villa-Trevino,2004Ardisia compressa.]]>42509516De Mejia, E.G., S. Chandra, M. Ramirez-Mares and W. Wang,2006Ardisia compressa extracts and their effect on human colon cancer cells.]]>4411911203Greenwood, D.,19891989pp: 91-100pp: 91-100Jannatipour, M., Y.X. Liu and J.L. Nitiss,19932681858618592Kang, Y.H., W.H. Kim, M.K. Park and B.H. Han,2001Ardisia crispa.]]>93736740Kobayashi, H. and E. de Mejia,200596347354Mann, C.D., C.P. Neal, G. Garcea, M.M. Manson, A.R. Dennison and D.P. Berry,2009181325Nikaido, H. and M. Vaara,198549132Nikolovska-Coleska, Z., L. Xu, Z. Hu, Y. Tomita and P. Li et al.,20044724302440Nitiss, J.L. and J.C. Wang,19888575017505Nitiss, J.L. and K.C. Nitiss,200195315327Nostro, A., M.P. Germano, V. D'Angelo, A. Marino and M.A. Cannatelli,200030379384Ralph, S.J., S. Rodriguez-Enriquez, J. Neuzil, E. Saavedra and R. Moreno-Sanchez,201031145170Ramirez-Mares, M.V., S. Fatell, S. Villa-Trevino and E.G. de Mejia,1999Ardisia compressa against benomyl-induced cytotoxicity and genotoxicity in cultured rat hepatocytes.]]>13889896Ramirez-Marez, M.V. and E.G. de Mejia,20034115271535Ramirez-Mares, M.V., S. Chandra and E.G. de Mejia,2004In vitro chemopreventive activity of Camellia sinensis, Ilex paraguariensis and Ardisia compressa tea extracts and selected polyphenols.]]>5545365Schweizer, F.,2009625190194Shureiqi, I., P. Reddy and D.E. Brenner,200033157167Siddhuraju, P. and K. Becker,2003Moringa oleifera Lam.) leaves.]]>5121442155Siddiqui, I.A., M. Saleem, Y.M. Adhami, M. Asim and H. Mukhtar,20072813921408Sumino, M., T. Sekine, N. Ruangrungsi, K. Igarashi and F. Ikegami,2002Ardisia colorata.]]>5014841487Hsieh, C.Y and S.T. Chang,20105815781583