Pakistan Journal of Nutrition1680-51941994-7984Asian Network for Scientific Information10.3923/pjn.2009.578.581Ali KambohAsghar RajputN. RajputI.R. KhaskheliM. KhaskheliG.B. 5200985In present study, 40 livestock vaccines were tested for bacterial contaminants. Four different bacterial species were identified from the vaccine samples. The species were Escherichia coli, Pasteurella multocida, Bacillus cereus and Bacillus subtilis. Of the 40 livestock vaccines studied, 1 Haemorrhagic septicaemia (H.S) and 2 Anthrax vaccines were found positive for bacterial contaminants, possessing batch numbers 057, 079 and 010 respectively, while 37 samples were observed without any bacterial growth. The percentage prevalence of positive vaccine samples was recorded as 7.5%. The pure contamination was recorded in 1 (33.33%) Anthrax vaccine sample with batch number 079, while 2 (66.67%) samples, 1 H.S and 1 Anthrax with batch numbers 057 and 010 respectively were recorded for mixed bacterial species. During investigating biochemical properties, it was observed that Escherichia coli show the positive reaction to catalase, and negative to oxidase, urease and indole. While Pasteurella multocida, Bacillus cereus and Bacillus subtilis were positive to catalase and oxidase, while negative to urease and methyl red.]]>Devrajani, K.,20052005Dewani, P.,20002000Fazlani, S.A.,20052005Khalil, M.A. and A. Gabbar,19922nd Edn.,pp: 6-93pp: 6-93Khan, T.S. and R. Rind,20014696702Kojima, A., T. Takahashi, M. Kijima, Y. Ogikubo and M. Nishimura et al.,1997Mycoplasma in avian live virus vaccines by polymerase chain reaction.]]>25365371Landman, W.J.M., K.T. Veldman, D.J. Mevius and P. Doornenbal,2000292125Mbulu, R.S., G. Tjipura-Zaire, R. Lelli, J. Frev and P. Pilo et al.,2004Mycoplasma mycoides subsp. Mycoides SC.]]>98229234Merchant, I.A. and R.A. Packer,19998th Edn.,pp: 386-387pp: 386-387Nicklin, J., K. Graeme-Cook, T. Paget and R.A. Killington,1999pp: 176-179pp: 176-179Nizamani, A.W.,19991999Samad, A.,20012001Tizard, I.R.,19954th Edn.,pp: 178-191pp: 178-191Tortora, J.G., R.B. Funke and L.C. Case,20017th Edn.,pp: 501-505pp: 501-505West, P.G.,199818th Edn.,pp: 581pp: 581