Pakistan Journal of Nutrition1680-51941994-7984Asian Network for Scientific Information10.3923/pjn.2009.242.250Bacillus megaterium 19 Strain]]>KhalilRowaida ElbahloulYasser DjadouniFatima OmarSanaa 3200983A Bacillus megaterium 19strain isolated
from a mixture of fermented vegetable wastes, produced a bacteriocin that
displayed a wide spectrum antimicrobial activity against food-spoilage
microorganisms and possessed a bactericidal mode of action. The bacteriocin
activity against Salmonella typhimurium and Staphylococcus aureus
attained its maximum value during the mid stationary phase. Maximal bacteriocin
activity (93% growth reduction) was achieved using MRS broth, at initial
pH of 5.5-6.5 and at 30°C incubation temperature. High levels of bacteriocin
activity (up to 85% growth reduction) were detected under limited or reduced
oxygen levels. The inhibitory activity increased in mMRS broth with 4%
sucrose and (0.10%) beef extract. The bacteriocin was thermally stable
over a wide temperature range up to 100°C for 15 min and retained
its activity at acidic and neutral pH values, but not at alkaline pHs.
Full bacteriocin activity was expressed after 30 days of storage at 4°C,
for 15 min of exposure to UV light. The bacteriocin was amylase and lipase
sensitive, however, treatment with proteolytic enzymes resulted in a remarkable
stability. SDS-PAGE analysis of the partially purified bacteriocin revealed
an apparent molecular weight ranging from 3.496 to 6.512 kDa. The strain
may have a potential use as food biopreservative, because of its promising
thermostable technological properties and broad antimicrobial spectrum.]]>Aasen, I.M., T. Moreto, T. Katla, L. Axelsson and I. Storro,2000Lactobacillus sake CCUG 42687.]]>53159166Abada, E.A.,2008Bacillus coagulans.]]>124146Albano, H., S.D. Todorov, C.A. van Reenen, T. Hogg, L.M. Dicks and P. Teixeira,2007Pediococcus acidilactici isolated from Alheira a fermented sausage traditionally produced in Portugal.]]>116239247Bizani, D. and A. Brandelli, 2002Bacillus sp. strain 8A.]]>93512519Cherif, A., H. Ouzari, D. Daffonchio, H. Cherif and K.B. Slama et al.,2001Bacillus thuringiensis BMG1.7, a new strain isolated from soil.]]>32243247De Kwaadsteniet, M., S.D. Todorov, H. Knoetze and L.M. Diks,2005Enterococcus mundtii ST15, with activity against gram-positive and gram-negative bacteria.]]>105433444De Kwaadsteniet, M., T. Fraser, C.A. van Reenen and L.M.T. Dicks,2006Enterococcus faecium T8, isolated from vaginal secretions of children infected with human immunodeficiency virus.]]>7247614766Delmar, B., S. Amanda, J. Motta, R. Morrissy, A. Terra, A. Souto and A. Brandelli,2005Bacillus cereus.]]>8125131Diop, M.B., R. Dubois-Dauphin, E. Tine, A. Ngom, J. Destain and P. Thonart,200711275281Karaoglu, A.S., F. Aydin, S.S. Kilic and A.O. Kilic,200333713Korenblum, E., I. von der Weid, A.L.S. Santos, A.S. Rosado and G.V. Sebastian et al.,2005Bacillus subtilis LFE-1, B. firmus H2O-1 and B. licheniformis T6-5 isolated from an oil reservoir in Brazil.]]>98667675Lasta, S., Z. Fajloun, H. Darbon, P. Mansuelle and N. Andreotti et al.,2008Lactococcus lactis subsp. cremoris J46 strain.]]>618993Lewus, C.B., A. Kaiser and T.J. Montville,1991816831688Line, J.E., E.A. Svetoch, B.V. Eruslanov, V.V. Perelygin and E.V. Mitsevich et al.,20085210941100Lisboa, M.P., D. Bonatto, D. Bizani, J.A. Henriques and A. Brandelli, 2006Bacillus amyloliquifaciens isolated from the Brazilian atlantic forest.]]>9111118Maidak, B.L., J.R. Cole, C.T. Parker, G.M. Jr. Garitty and N. Larsen et al.,199927171173Martin-Visscher, L.A., M.J. van Belkum, S. Garneau-Tsodikova, R.M. Whittal, J. Zheng, L.M. McMullen and L. Vederas,2008Carnobacterium maltaromaticum UAL307.]]>7447564763Montville, T.J., K. Winkowski and R.D. Lusdescher,19955797814Naclerio, G., E. Ricca, M. Sacco and M. de Felice, 1993Bacillus cereus. ]]>5943134316Ogunbanwo, S.T., A.I. Sanni and A.A. Onilude,2003Lactobacillus plantarum F1 and Lactobacillus brevis OG1.]]>2219227Ogunbanwo, S.T., A.I. Sanni and A.A. Onilude, 2003Lactobacillus brevis OG1.]]>27179184Pattnaik, P., J.K. Kaushik, S. Grover and V.K. Batish, 2001Bacillus licheniformes isolated from water buffalo.]]>91636645Sambrook, J. and D.W. Russell,20013rd Edn.,pp: 131-138pp: 131-138Schillinger, U. and F.K. Lucke,19874199208Svetoch, E.A., N.J. Stern, B.V. Eruslanov, Y.N. Kovalev and L.I. Volodina et al.,2005Bacillus circulans and Paenibacillus polymyxa strains inhibitory to Campylobacter jejuni and characterization of associated bacteriocins.]]>6811117Brink, B.T., M. Minekus, J.M.B.M. van der Vossen, R.J. Leer and J.H.J.H. Intveld,1994Lactobacillus acidophilus M46.]]>77140148Todorov, S.D., S.T. Danova, C.A. van Reenen, M. Meincken, G. Dinkova, I.V. Ivanova and L.M. Dicks,2006Lactococcus lactis subsp. lactis HV219 isolated from human vaginal secretions.]]>46226238Todorov, S.D. and L.M. Dicks,2006Lactobacillus plantarum strains ST23LD and ST341LD, isolated from spoiled olive brine.]]>411119Verluyten, V., W. Messens and L. de Vuyst,2003Lactobacillus curvatus LTH 1174 under anaerobic conditions.]]>6938333839Vinderola, C.G., G.A. Costa, S. Regenhardt and J.A. Reinheimer,200212579589Vinderola, C.G., P. Mocchiutti and J.A. Reinheimer, 200285721729Joshi, V.K., S. Sharma and N.S Rana,200644435439Lyon, W.J. and B.A. Glatz, 1991Propionibacterium thoenii.]]>57701706Aktypis, A., G. Kalantzopulous, J.H.J. Huis in't Veld and B. ten Brink,1998Streptococcus thermophilus ACA-DC 0040.]]>84568576