Pakistan Journal of Nutrition1680-51941994-7984Asian Network for Scientific Information10.3923/pjn.2009.1048.1051KhampaSittisak ChaowaratPala KoatdokeUthai SinghalertRungson WanapatMetha 7200987Four, one-year old of native cattle were randomly assigned according to a 2x2 Factorial arrangement in a 4x4 Latin square design to study supplementation of malate level at 500 and 1,000 g with yeast (Saccharomyces cerevisiae) at 1,000 and 2,000 g in concentrate containing high levels of cassava chip. The treatments were as follows: T1 = supplementation of malate at 500 g with yeast at 1,000 g; T2 = supplementation of malate at 500 g with yeast at 2,000 g; T3 = supplementation of malate at 1,000 g with yeast at 1,000 g; T4 = supplementation of malate at 1,000 g with yeast at 2,000 g in concentrate, respectively. The animals were offered the treatment concentrate at 1% BW of DM and urea-treated rice straw was fed ad libitum. The results revealed that concentration of volatile fatty acid was significantly different especially the concentration of propionic acid was slightly higher in cattle receiving T4 than T3, T2 and T1 (23.3, 21.9, 20.9 and 18.0%, respectively). The populations of protozoa and fungal zoospores were significantly different as affected by malate level and yeast. In conclusion, the combined use of concentrate containing high level of cassava chip at 70% DM with malate at 1,000 g and yeast at 2,000 g in concentrate with urea-treated rice straw as a roughage could improved rumen ecology in native cattle.]]>AOAC,1985Brossard, L., F. Chaucheyras-Dur, B. Michalet-Doreau and C. Martin,200682829836Callaway, T.R. and S.A. Martin,1996in vitro mixed ruminal microorganism fermentation of cracked corn.]]>7419821989Chanjula, P., M. Wanapat, C. Wachirapakorn, S. Uriyapongson and P. Rowlinson,200316211216Chanjula, P., M. Wanapat, C. Wachirapakorn, S. Uriyapongson and P. Rowlinson,20041714001410France, J. and R.C. Siddons,19931993pp: 196-203pp: 196-203Galyean, M.,19891st Edn.,pp: 162-167pp: 162-167Gottschalk, G.,19862nd Edn.,Guedes, C.M., D. Goncalves, M.A.M. Rodrigues and A. Dias-da-Silva,2007Saccharomyces cerevisiae yeast on ruminal fermentation and fibre degradation of maize silages in cows.]]>1452740Khampa, S., M. Wanapat, C. Wachirapakorn, N. Nontaso, M.A. Wattiaux and P. Rowlison,200619368375Martin, S.A., M.N. Streeter, D.J. Nisbet, G.M. Hill and S.E. Williams,19997710081015Newbold, C.J., R.J. Wallace and F.M. McIntosh,1996Saccharomyces cerevisiae as a feed additive for ruminants.]]>76249261Sanson, D.W. and O.T. Stallcup,19843012611267SAS,1998Version 6.12,Sommart, K., M. Wanapat, D.S. Parker and P. Rowlinson,20001310941101Steel, R.G.D. and J.H. Torrie, 1980Wanapat, M.,200316463472Wanapat, M. and O. Pimpa,19993-N levels on ruminal fermentation, purine derivatives, digestibility and rice straw intake in swamp buffaloes.]]>12904907Goering, H.K. and P.J. van Soest,19701970