Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2022.15.22ChatuphonprasertWaranya Tukum-meeWipawee WattanathornJintanaporn JarukamjornKanokwan 12022251Background and Objective: Pineapple (Ananas comosus) is a popular fruit worldwide with natural antioxidant properties. This study examined how pineapple modified the expression of drug-metabolizing enzymes (CYP1A2, CYP2C9, CYP3A4, UGT1A6, NAT2 and SULT1A1) and a drug transporter (OATP1B1) in human hepatocarcinoma (HepG2) cells. Materials and Methods: HepG2 cells (2.5×105 cells/well in a 24-well plate) were incubated with pineapple juice extract (125-1,000 μg mL1) for 48 hrs in phenol red-free medium. Resazurin reduction, ROS, AST and ALT assays were performed. The mRNA expression of target genes was determined by RT/qPCR. Results: Pineapple juice slightly reduced HepG2 cell viability to 80% of the control, while ROS, AST and ALT levels were not changed. Pineapple juice did not alter the expression of CYP1A2, CYP2C9 and UGT1A6 mRNA. All tested concentrations of pineapple juice suppressed CYP3A4, NAT2 and OATP1B1 expression, while SULT1A1 expression was induced. Conclusion: Though pineapple juice slightly decreased the viability of HepG2 cells, cell morphology and cell function remained normal. Pineapple juice disturbed the expression of phase I (CYP3A4) and phase II (NAT2 and SULT1A1) metabolizing genes and the drug transporter OATP1B1. Therefore, the consumption of excessive amounts of pineapple juice poses a risk for drug interactions.]]>Das, G., J.K. Patra, T. Debnath, A. Ansari and H.S. Shin,2019Ananas comosus (L.).]]>2019Wali, N.,2019Ananas comosus).]]>2019pp: 367-373pp: 367-373Bamidele, O.P. and M.B. Fasogbon,2017Trichosanthes cucumerina) juice and pineapple (Ananas comosus) juice blends and their changes during storage.]]>220184189Kargutkar, S. and S. Brijesh,2018Ananas comosus.]]>26469477Secor, E.R., S.M. Szczepanek, C.A. Castater, A.J. Adami and A.P. Matson et al.,20132013Zanger, U.M. and M. Schwab,2013138103141Sasaki, T., Y. Sato, T. Kumagai, K. Yoshinari and K. Nagata,20172017Nishikawa, M., N. Ariyoshi, A. Kotani, I. Ishii and H. Nakamura et al.,200419280289Kim, S.B., K.S. Kim, D.D. Kim and I.S. Yoon,2019110111117Salazar-González, R.A., E. Turiján-Espinoza, D.W. Hein, R.C. Milán-Segovia, E.E. Uresti-Rivera and D.P. Portales-Pérez,2018156340347Rasool, M.I., A.F. Bairam, S.A. Gohal, A.A.E. Daibani and F.A. Alherz et al.,201971257265Lněničková, K., M. Šadibolová, P. Matoušková, B. Szotáková, L. Skálová and I. Boušová,20202020Kim, S.B., I.S. Yoon, K.S. Kim, S.J. Cho and Y.S. Kim et al.,2014In vitro and in vivo evaluation of the effect of puerarin on hepatic cytochrome P450-mediated drug metabolism.]]>80561567Kayesh, R., T. Farasyn, A. Crowe, Q. Liu and S. Pahwa et al.,2021110314324Chen, L., L. Liu, Y. Chen, M. Liu and Y. Xiong, 2019Radix ophiopogonis.]]>4912211228Donato, M.T., L. Tolosa and M.J. Gómez-Lechón,20152015pp: 77-93pp: 77-93Chatuphonprasert, W. and K. Jarukamjorn,2012329941001Sriset, Y., W. Chatuphonprasert and K. Jarukamjorn,2021In vitro antioxidant potential of Mallotus repandus (Willd.) Muell. Arg stem extract and its active constituent bergenin.]]>432430Chatuphonprasert, W., T. Kitisripanya, W. Putalun, I. Ellinger and K. Jarukamjorn,2020Pueraria candollei var. mirifica-induced CYP1A1 and CYP1A2 expression in human choriocarcinoma bewo cells.]]>16506512Sriset, Y., W. Chatuphonprasert and K. Jarukamjorn,20211197115Sriset, Y., W. Chatuphonprasert and K. Jarukamjorn,20202020Novotna, A., M. Korhonova, I. Bartonkova, A.A. Soshilov and M.S. Denison et al.,20142014Van den Bergh, A., J. Snoeys, L. De Zwart, P. Ward and A. Lopez-Gitlitz et al.,20205911491160Nikulin, S.V., E.A. Tonevitsky and A.A. Poloznikov,201766150155Yokobori, K., K. Kobayashi, I. Azuma, H. Akita and K. Chiba,201732265272Präbst, K., H. Engelhardt, S. Ringgeler and H. Hübner,20172017pp: 1-17pp: 1-17O'Brien, J., I. Wilson, T. Orton and F. Pognan,200026754215426Ivanova, N.N., L.M. Khomich, I.B. Perova and K.I. Eller,2019887382Uetaki, M., S. Tabata, F. Nakasuka, T. Soga and M. Tomita,20152015Husain, A., X. Zhang, M.A. Doll, J.C. States, D.F. Barker and D.W. Hein,200735721727Kukongviriyapan, V., N. Phromsopha, W. Tassaneeyakul, U. Kukongviriyapan, B. Sripa, V. Hahnvajanawong and V. Bhudhisawasdi,2006361528Miksits, M., A. Maier-Salamon, S. Aust, T. Thalhammer and G. Reznicek et al.,20053511011119Wang, L. and D.H. Sweet,20128410881095