Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2022.1077.1084Trametes versicolor (L.) Lloyd in Submerged Fermentation]]>YulianaT. PutriR. AshifaHanidahI. MardawatiE. TjaturinaH. 1220222512Background and Objective: Laccase is classified as an oxidoreductase enzyme that catalyzes oxidation reactions of phenolic groups by using oxygen as its electron acceptor. Laccase isolated from Trametes versicolor (L.) Lloyd has a wide range of applications in the industrial sector. The use of enzymes in the industrial sector requires pure enzyme conditions from impurities so that the enzyme can maximize its ability in converting the substrate. This study aims to obtain enzyme activity and the characteristic of purified laccase enzymes isolated from Trametes versicolor (L.) Lloyd. Materials and Methods: This study was conducted with an experimental method followed by descriptive analysis. The steps of this research consist of a qualitative assay of laccase enzyme, crude laccase extract desalting by Sephadex G-25, laccase purification by Sephadex G-100 and laccase optimum pH characterization. Results: The result of this study showed that purification of laccase from Trametes versicolor (L.) Lloyd with Sephadex G-25 increases laccase enzyme-specific activity which is 10.966 U mg1 and reaches 2.93-fold purity. The highest laccase enzyme activity was achieved at pH 4 with a value of laccase activity 62.39 U L1. Conclusion: Based on current results, purifying laccase from Trametes versicolor (L.) Lloyd with Sephadex G-25 was recommended which resulting higher enzyme specific activity.]]>Khootama, A., D.N. Putri and H. Hermansyah,2018Aspergillus niger using agro-industrial waste by solid state fermentation.]]>153143148Zouari-Mechichi, H., T. Mechichi, A. Dhouib, S. Sayadi, A.T. Martinez and M.J. Martinez,2006Trametes trogii isolated in Tunisia: Decolorization of textile dyes by the purified enzyme.]]>39141148Kudanga, T. and M.L. Roes-Hill,20149865256542Fillat, A., J.F. Colom and T. Vidal,201010141044110Järvinen, J., S. Taskila, R. Isomäki and H. Ojamo,20122012Rodríguez-Couto, S.,20178456466Kunamneni, A., I. Ghazi, S. Camarero, A. Ballesteros, F.J. Plou and M. Alcalde,200843169178Yang, J., Q. Lin, T.B. Ng, X. Ye and J. Lin,2014Cerrena sp. HYB07 with dye decolorizing ability.]]>2014Minussi, R.C., G.M. Pastore and N. Duran,200213205216Stanbury, P., A. Whitaker and S.J. Hall,20163rd Edn.,Pages: 824Pages: 824Han, M.L., J. Yang, Z.Y. Liu, C.R. Wang and S.Y. Chen et al.,20212021Rittstieg, K., A. Suurnakki, T. Suortti, K. Kruus, G.M. Guebitz and J. Buchert,2003O-4-substructure by Trametes hirsuta laccase in the presence of ABTS.]]>1915051509Bugg, T.D.H., 20123rd Edn.,Pages: 304Pages: 304Ang, T.N., G.C. Ngoh and A.S.M. Chua,20116589595Liu, J., Y. Cai, X. Liao, Q. Huang, Z. Hao, M. Hu and D. Zhang,2013Pycnoporus sp. sybc-L3 and its use in dye decolorisation.]]>113B2739Vantamuri, A.B. and B.B. Kaliwal,2016Marasmius species BBKAV79 and effective decolorization of selected textile dyes.]]>2016Buswell, J.A., Y. Cai and S.T. Chang,1995Lentinula (Lentinus) edodes.]]>1288187Bradford, M.M.,197672248254Özşölen, F., P. Aytar, S. Gedikli, M. Çelikdemir, M. Ardiç and A. Çabuk,201046978Senthivelan, T., J. Kanagaraj, R.C. Panda and T. Narayani,2019Penicillium chrysogenum: Media optimization by response surface methodology (RSM) and central composite rotatable design (CCRD).]]>2019Umar, A. and S. Ahmed,2022Ganoderma leucocontextum along with its phylogenetic relationship.]]>2022Rochefort, D., D. Leech and R. Bourbonnais,200461424Younes, S.B., T. Mechichi and S. Sayadi,2007Perenniporia tephropora and its role in the decolourization of synthetic dyes.]]>10210331042Ó’Fágáin, C., P.M. Cummins and B.F. O’Connor,20112011pp: 25-33pp: 25-33Yaropolov, A.I., O.V. Skorobogat'ko, S.S. Vartanov and S.D. Varfolomeyev,199449257280Taha, A.A., I.I. Shwaish, A.H. Mohammed, A.J. Haider and H. Stamatis,2013Botrytis cinerea (DSMZ 877) and application for textile phenolic dye decolorization.]]>36862871Kumar, A., A.K. Singh, S. Ahmad and R. Chandra, 2020Bacillus sp. strain AKRC01 in presence of agro-waste as effective substrate using response surface methodology.]]>14351362Garcia, T.A., M.F. Santiago and C.J. Ulhoa,2006Pycnoporus sanguineus induced by 2,5-xylidine.]]>28633636Iyer, G. and B.B. Chattoo,2003Magnaporthe grisea.]]>227121126Fithri, L., N.N.T. Puspaningsih, O. Asmarani, Ni'matuzahroh, G.D.F. Dewi and R.Y. Arizandy,20202020Almowallad, S.A., G.M. Alshammari, M.M. Alsayadi, N. Aljafer, E.A. Al-Sanea, M.A. Yahya and L.N. Al-Harbi,2022Penicillium oxalicum AUMC 4153.]]>2022Ramos, O.L. and F.X. Malcata,20172017pp: 587-603pp: 587-603Hildén, K., T.K. Hakala and T. Lundell,20093111171128Góralczyk-Bińkowska, A., A. Jasińska, A. Długoński, P. Płociński and J. Długoński,2020Nectriella pironii and innovative strategies for its production on leaf litter of an urban park.]]>2020Jin, X., X. Yu, G. Zhu, Z. Zheng, F. Feng and Z. Zhang,20162016Hossain, S.K.M. and N. Anatharaman,2006Trametes versicolor with bagasse powder.]]>5189194Nelson, D.L., A.L. Lehninger and M.M. Cox,20084th Edn.,Pages: 1158Pages: 1158Bhagavan, N.V. and C.E. Ha,20152015 pp: 63-84 pp: 63-84