Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2021.374.382Trametes versicolor Strains Using Sawdust and Rice Husk-based Substrate]]>Thuy Thi NguyenBich LeVe Van Trang Thi NguyenHuyen Thi NguyenLuyen Dong TranAnh Xuan NgoNghien 32021243Background and Objective:Trametes versicolor has not only been valued in medical use but also in environmental protection. One of the major challenges currently faced in the commercial cultivation of T. versicolor is finding superior strains that can produce high yields. In an attempt to search for high-yield potential T. versicolor, two wild strains, namely VNUA and BV, were isolated and evaluated for potential cultivation. Materials and Methods: Optimized culture conditions were set up by one-individual factor-at-a-time. Four different kinds of culture media, including Czapek, Raper, PGA and modified Potato Dextrose Agar (PDA), were investigated to ascertain the optimal media. The efficiency of sawdust and rice grain for mother spawn production was evaluated. Different combinations of sawdust and rice husk were tested to investigate the most favorable substrate mixtures. Results: The ideal medium and temperature for the favorable mycelial growth of T. versicolor were PGA and 30°C, respectively. The optimal spawning material for upscaling of the mycelium was Treatment D (20% rice grain, 79% sawdust and 1% calcium carbonate). The strains were successfully cultivated in a basal substrate combination of sawdust and rice husk supplemented with wheat bran. Investigated strains responded differently to different substrates cultivation. Of note, compared with strain BV, strain VNUA showed a significantly higher biological efficiency (7.3%). Conclusion: Wild T. versicolor strains were successfully fructified under artificial cultivation conditions. Strain VNUA can be considered as a potential strain for commercial cultivation. The use of sawdust for the spawn production of T. versicolor can reduce the cost of manufacturing.]]>Jo, W.S., M.J. Kang, S.Y. Choi, Y.B. Yoo, S.J. Seok and H.Y Jung, 2010Coriolus Versicolor.]]>38195202Pop, R.M., I.C. Puia, A. Puia, V.S. Chedea, N. Leopold, I.C. Bocsan and A.D. Buzoianu,2018Trametes versicolor: Medicinal mushroom with important health benefits.]]>46343349Habtemariam, S.,2020Trametes versicolor (Synn. Coriolus versicolor) polysaccharides in cancer therapy: Targets and efficacy.]]>2020Chauhan, P.S., M. Kumarasamy, A. Sosnik and D. Danino, 2019113943639448Hsieh, T.C. and J.M. Wu, 2013Coriolus versicolor and Ganoderma lucidum.]]>32251257Wang, D.F., N. Lou and X.D. Li, 2012Coriolus versicolor polysaccharide-B on the biological characteristics of human esophageal carcinoma cell line Eca109. ]]>9164167Maruyama, S., T. Akasaka, K. Yamada and H. Tachibana, 200963409412Yang, J., W. Li, T.B. Ng, X. Deng, J. Lin and X. Ye,20172017Aguiar, A., D. Gavioli and A. Ferraz,20143+-reducing activity and wood degradation by the white-rot fungus Trametes versicolor ATCC 20869.]]>118935942Marco-Urrea, E., M. Pérez-Trujillo, T. Vicent and G. Caminal, 2009Trametes versicolor.]]>74765772Mallak, A.M., A. Lakzian, E. Khodaverdi, G.H. Haghnia and S. Mahmoudi,2020Pleurotus ostreatus and Trametes versicolor on triclosan biodegradation and activity of laccase and manganese peroxidase enzymes.]]>2020Atilano-Camino, M.M., L.H. Álvarez-Valencia, A. García-González and R.B. García-Reyes, 2020Trametes versicolor using lignocellulosic residues as cosubstrates and evaluation of enzymes for blue wastewater biodegradation.]]>2020Xu, L., K. Sun, F. Wang, L. Zhao, J. Hu, H. Ma and Z. Ding, 2020Trametes versicolor in solid-state fermentation using tea residues as substrate and its application in dye decolorization.]]>2020Guerrero, D.G., V.E. Martinez and R. de la T Almaraz, 2011Trametes versicolor in Mexico. ]]>23 5558Nguyen, B.T.T., N.X. Ngo, V.V. Le, L.T. Nguyen, R. Kana and H.D. Nguyen,2019616267Pekşen, A. and B. Kibar, 2020Macrolepiota procera mushroom.]]>191120Luangharn, T., S.C. Karunarathna, P.E. Mortimer, K.D. Hyde, N. Thongklang and J. Xu, 2019Ganoderma tropicum (Basidiomycota, Polyporales) for Thailand and first assessment of optimum conditions for mycelia production.]]>516583Dulay, R.M.R. and E.J.B. Garcia, 2017Lentinus strigosus (BIL1324).]]>12323328Kang, J.A., K.H. Ka, J.Y. Kim and S.H. Kim, 201846281294Snajdr, J. and P. Baldrian,2007Pleurotus ostreatus and Trametes versicolor.]]>52498502Rizal, L.M., K.D. Hyde, E. Chukeatirote, S.C. Karunarathna, P. Kakumyan and S. Chamyuang,2016Macrolepiota dolichaula in Thailand.]]>43959971Nguyen, L.T., V.V. Le, B.T.T. Nguyen, N.X. Ngo, H.T.T. Nguyen, Q.D. Nguyen and S. Mulla, 2020Cordyceps militaris strains under artificial cultivation conditions. ]]>101135145Nguyen, T.M. and S.L. Ranamukhaarachchi,2020Pleurotus eryngii and Pleurotus ostreatus.]]>23223230Elhami, B. and N. Alemzadeh Ansari,2008mushroom species.]]>8474477Zhang, R.Y., D.D. Hu, X.T. Ma, S.G. Li, J.G. Gu and Q.X. Hu, 2014Pleurotus eryngii.]]>175156159Zhang, W.R., S.R. Liu, Y.B. Kuang and S.Z. Zheng, 2019Pleurotus ostreatus, in liquid culture and its cultivation evaluation.]]>4797104Oyetayo, V.O. and O.O. Ariyo,2013Pleurotus ostreatus (Jacq: Fries) cultivated on different wood substrates.]]>6223226Owaid, M.N., A.M. Abed and B.M. Nassar,2015Pleurotus ostreatus in Iraq.]]>27537541Narain, R., R.K. Sahu, S. Kumar, S.K. Garg, C.S. Singh and R.S. Kanaujia,2008Pleurotus florida on maize cobs substrate.]]>2917Gaitán-Hernández, R., E.N. Aquino-Bolaños, M. Herrera and D. Salmones, 202032188197Yildiz, S., U.K. Yildiz, E.D. Gezer and A. Temiz,2002Pleurotus ostreatus culture mushroom.]]>38301306Rizki, M. and Y. Tamai,2011Pleurotus ostreatus).]]>2716951702