Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2021.165.171Zanthoxylum acanthopodium) Fruits in NOTCH1 and Hes1 Expressions to Human Placental Trophoblasts]]>C. SitumorangPutriIlyasSyafruddinHutahaeanSalomoRosidah12021241Background and Objective: Andaliman fruit (Zanthoxylum acanthopodium) is a well-known spice antioxidant in Northern Sumatera (Indonesia). The cellular activity requires antioxidants in counteracting free radicals. The cellular proteins that play a role in development, proliferation, differentiation and embryonic processes in the human placenta are NOTCH1 and Hes1. The aim of this research was to analyze the expression of NOTCH1 and Hes1 genes after administering nano herbal andaliman to the trophoblast cells of the human placenta. Materials and Methods: HTR8 trophoblast cells were divided into two groups, namely, control and treatment (nano herbal andaliman). RNA isolation, reverse transcription and RT-PCR (real-time polymerase chain reaction) were performed to analyze the NOTCH1 and Notch target gene (Hes1) expressions. The NOTCH1 and Hes1 gene expressions were quantified using the CT method (2-ΔΔCT) and normalized with Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene expressions. Results: Nanoherbal andaliman reduced the expression of NOTCH1 genes in the human placental trophoblast. However, it increased the expression of Hes1 when the incubation time was 16 hrs. Conclusion: Nanoherbal andaliman decreases the expression of genes that are crucial in hypoxia and free radicals in the placenta, namely, NOTCH1 and Hes1 increased after incubation for 16 hrs. Therefore, this herb needs to be evaluated further.]]>Wijaya, C.H., F.I. Napitupulu, V. Karnady and S. Indariani,2019Zanthoxylum acanthopodium DC.).]]>Food Rev. Int.,35119Yanti, T.E. Pramudito, N. Nuriasari and K. Juliana,2011Zanthoxylum acanthopodium DC.) suppresses the expression of inflammatory mediators in lipopolysaccharide-induced macrophages in vitro.]]>Am. J. Biochem. Biotechnol.,7190195Gultom, S., I.H. Suparto and I. Batubara,2011Zanthoxylum acanthopodium fruit as an antioxidant and α-glucosidase inhibitors.]]>Flavonoid of Zanthoxylum acanthopodium fruit as an antioxidant and α-glucosidase inhibitors.2011Situmorang, P.C., S. Ilyas and S. Hutahaean,2019Zanthoxylum acanthopodium) and Extra Virgin Olive Oil (EVOO) effects in the expression of Malondialdehyde (MDA), Heat Shock Protein-70 (HSP70) and placental histology of preeclamptic rats.]]>Pharm. Sci.,25205220Situmorang, P.C., S. Ilyas, S. Hutahaean and R. Rosidah,2019Zanthoxylum acanthopodium) and extra virgin olive oil combination on preeclamptic rats liver histology.]]>Open Access Maced. J. Med. Sci.,722262231Situmorang, P.C., S. Ilyas, S. Hutahaean, Rosidah and R.D. Manurung,2020Zanthoxylum acanthopodium). ]]>Rasayan J. Chem., 13780788Situmorang, P.C., S. Ilyas and S. Hutahaean,2019Zanthoxylum acanthopodium DC.) and Extra Virgin Olive Oil (EVOO) to kidney histology of preeclampsia rats.]]>IOP Conf. Ser.: Earth Environ. Sci., Vol. 305, No. 1.2019Sarikaya, D.P. and L.A. Jerome‑Majewska,2011Notch1 and the activated NOTCH1 intracellular domain are expressed in differentiated trophoblast cells.]]>Cell. Biol. Int.,35443447Cormier, S., S. Vandormael-Pournin, C. Babinet and M. Cohen-Tannoudji,2004Gene Expression Patterns,4713717Yu, Y., L. Wang, W. Tang, D. Zhang and T. Shang, 2014Acta Histochem.,116911919Wang, Z., Y. Li, S. Banerjee, D. Kong and A. Ahmad et al.,2010J. Cell. Biochem.,109726736Sahlgren, C., M.V. Gustafsson, S. Jin, L. Poellinger and U. Lendahl, 2008Proc. Natl. Acad. Sci.,10563926397De Falco, M., L. Cobellis, D. Giraldi, A. Mastrogiacomo and A. Perna et al.,2007Placenta,28118126Liu, Z.J., T. Shirakawa, Y. Li, A. Soma and M. Oka et al.,2003Mol. Cel. Bio., 231425Shawber, C.J. and J. Kitajewski,2004Bioessays,26225234Bai, G., N. Sheng, Z. Xie, W. Bian and Y. Yokota et al.,2007Hes1 expression to inhibit precocious neurogenesis by releasing negative autoregulation of Hes1. ]]>Dev. Cell,13283297Liu, Z.H., X.M. Dai and B. Du,2015Cancer Biol. Ther.,16353359Fre, S., M. Huyghe, P. Mourikis, S. Robine, D. Louvard and S. Artavanis-Tsakonas,2005Nature,435964968Campagnolo, L., C. Telesca, M. Massimiani, H. Stuhlmann and M. Angelico et al.,2016Digestive Liver Dis.,487680Yamagishi, S.I. and T. Matsui,2011Pharmacol. Res.,64187194Weng, A.P.,2004Science,306269271Velicky, P., S. Haider, G.R. Otti, C. Fiala, J. Pollheimer and M. Knöfler, 2014Mol. Hum. reprod., 20756766Hatakeyama, J.,2004Development,13155395550Nakamura, Y., S.I. Sakakibara, T. Miyata, M. Ogawa and T. Shimazaki et al.,2000Hes1 as a repressor of the neuronal commitment of CNS stem cells.]]>J. Neurosci.,20283293Stockhausen, M.T., J. Sjölund and H. Axelson,2005Exp. Cell. Res.,310218228Lee, S.H., H.S. Hong, Z.X. Liu, R.H. Kim, M.K. Kang, N.H. Park and K.H. Shin,2012Biochem. Biophys. Res. Com.,4245864