Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2021.1195.1201Plumbago indica Differentially Modulated Cytochrome P450 and Transporter Profiles in BeWo and HepG2 Cells]]>ChatuphonprasertWaranya SukkasemNadta EllingerIsabella JarukamjornKanokwan 1120212411Background and Objective: The medicinal herb Plumbago indica (PI) and its major constituent plumbagin have reported pharmacological properties but there is a lack of information about their herb-drug interactions. The effects of methanolic (PI-MeOH) and ethanolic (PI-EtOH) crude extracts of PI and plumbagin on the expression of cytochrome P450s (CYP1A2, CYP2E1 and CYP3A4) and transporters (ABCC1, ABCG2 and SLC22A11) were investigated in BeWo and HepG2 cells. Materials and Methods: BeWo or HepG2 cells were treated with 0.5-5 μM plumbagin or 25-500 μg mL1 of PI-MeOH or PI-EtOH for 24 hrs. Total RNA was extracted and mRNA expression of CYPs and transporters were determined using RT-qPCR. Results: PI and plumbagin affected mRNA expression differently in the two tested cell types. In BeWo cells, all concentrations of PI-MeOH induced CYP2E1, 100 and 500 μg Ml1 PI-MeOH and PI-EtOH up-regulated CYP1A2, CYP3A4 and ABCG2 and 500 μg mL1 PI-EtOH induced ABCG2 expression. Plumbagin suppressed CYP1A2 and induced SLC22A11 expression at the highest concentration, 5 μM. In HepG2 cells, 5 μM plumbagin and 500 μg Ml1 PI-EtOH suppressed CYP3A4 expression and 500 μg mL1 PI-MeOH and PI-EtOH up-regulated CYP1A2 and CYP2E1 expression. ABCC1 expression was induced by all treatments while ABCG2 and SLC22A11 were induced only by 500 μg mL1 PI-MeOH and PI-EtOH. Conclusion: The use of PI or plumbagin supplements in large quantities or for long periods should be carefully considered due to the risk of herbal drug interactions via modulated expression of CYPs and transporters.]]>Jayanthi, M., A. Gokulanathan, P. Haribalan, K. Ashakiran and C.D. Kumar et al.,20202020Dissanayake, D.M.I.H., D.D.B.D. Perera, L.R. Keerthirathna, S. Heendeniya, R.J. Anderson, D.E. Williams and L.D.C. Peiris,2020Plumbago indica and ligand screening of plumbagin against methicillin-resistant Staphylococcus aureus.]]>2020Chavan, R.D., P. Shinde, K. Girkar, R. Madage and A. Chowdhary,2016Plumbago indica and Allium sativum extracts.]]>8105111Chewchinda, S., P. Lomarat and P. Sithisarn,2018424550Sychev, D.A., G.M. Ashraf, A.A. Svistunov, M.L. Maksimov and V.V. Tarasov et al.,2018in vivo.]]>1211471156Zanger, U.M. and M. Schwab,2013138103141Gessner, A., J. König and M.F. Fromm,201910513861394Bruckmueller, H. and I. Cascorbi,2021]]>17369396Han, L.W., C. Gao and Q. Mao,201814817829Liu, X.,20192019pp: 1-12pp: 1-12Chatuphonprasert, W., K. Jarukamjorn and I. Ellinger,20182018Sukkasem, N., W. Chatuphonprasert and K. Jarukamjorn,2018Plumbago indica Linn. and plumbagin after oral administration in mice.]]>14507512Chatuphonprasert, W., N. Tatiya-aphiradee and K. Jarukamjorn,2015Plumbago indica Linn. and plumbagin on the expression of hepatic cytochrome P450 2e1 and lung cytochrome P450 2f2 in mice.]]>34692696Chen, S., Q. Wu, X. Li, D. Li and N. Mei et al.,2021396886Kallol, S., R. Moser-Haessig, C.E. Ontsouka and C. Albrecht,201872-734852Sriset, Y., W. Chatuphonprasert and K. Jarukamjorn,20191810011007Chatuphonprasert, W., T. Kitisripanya, W. Putalun, I. Ellinger and K. Jarukamjorn,2020Pueraria candollei var. mirifica-induced CYP1A1 and CYP1A2 expression in human choriocarcinoma bewo cells.]]>16506512Van den Bergh, A., J. Snoeys, L. De Zwart, P. Ward and A. Lopez-Gitlitz et al.,20205911491160Zhou, S.F., B. Wang, L.P. Yang and J.P. Liu,201042268354Caparrotta, T.M., D.J. Antoine and J.W. Dear,201774147160Robinson, J.F., E.G. Hamilton, J. Lam, H. Chen and T.J. Woodruff,20201003544Yin, W., D. Xiang, T. Wang, Y. Zhang and C.V. Pham et al.,20212021Kumarasamy, M. and A. Sosnik,20202020pp: 337-369pp: 337-369Noguchi, S., T. Nishimura, S. Mukaida, L.Z. Benet, E. Nakashima and M. Tomi,201710628952898Jana, S. and H. Rastogi,2017in vitro permeability, metabolism and in vivo pharmacokinetics of melatonin in rats: Potential for herb-drug interaction.]]>42781791