Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2021.1015.1021Eleutherine palmifolia Flower]]>RizaliAkhmad Laili AzizaNoor SariNoorkomala 1020212410Background and Objective: Endophytic fungi live in plant tissue and show no symptoms of disease in their host plants. It is known that endophytes as biological agents, can control plant diseases. In this study, isolated endophytic fungi from healthy Dayak Onion flowers were used as biocontrol agents in the control of the pathogenic fungi Fusarium spp. that causes molar disease in shallot plants. Materials and Methods: This study identifies the type of endophytic fungi molecularly isolated from Dayak onion flowers and determine the antagonistic effect of the endophytic extract against Fusarium spp. screening for endophytic fungi as antagonizing agents is carried out using the poisoned food method. Results: The results showed that two endophytic fungi isolates were obtained from healthy Dayak onion flowers, namely, EnI which was identified with the primers ITS1 and ITS4 as Fusarium solani and EnK as Neoscytalidium sp. Fusarium wilt caused by pathogenic fungi was identified as Fusarium oxysporum. The inhibitory percentage of EnI extract against the pathogenic fungus Fusarium oxysporum was 71.09% (high inhibition percentage) and the inhibition percentage of EnK was 38.54% (low inhibition percentage). Conclusion: Based on the results of this study, recommend using the endophytic fungus EnI extracts (Fusarium solani ), extracted from Dayak onion flowers to control the pathogen Fusarium oxysporum.]]>Dhayanithy, G., K. Subban and J. Chelliah,2019Catharanthus roseus.]]>2019Fatima, N., U. Mukhtar, Ihsan-Ul-Haq, M.A. Qazi, M. Jadoon and S. Ahmed,2016Chaetomium sp. NF15 of Justicia adhatoda L.: A potential candidate for drug discovery.]]>2016Idris, A.M., I. Al-Tahir and E. Idris,20132013Kumar, S. and N. Kaushik,2013Jatropha curcas produces oil and exhibit antifungal activity.]]>2013Afzal, I., Z.K. Shinwari, S. Sikandar and S. Shahzad,20192213649Venieraki, A., M. Dimou and P. Katinakis,2017105166Gao, F.K., C.C. Dai and X.Z. Liu,2010413461351Ibrahim, M., E. Oyebanji, M. Fowora, A. Aiyeolemi, C. Orabuchi, B. Akinnawo and A.A. Adekunle,20212021Shi, P., W. Du, Y. Wang, X. Teng, X. Chen and L. Ye,2019Eleutherine bulbosa (Mill.) Urb.]]>7148154Schulz, B., C. Boyle, S. Draeger, A.K. Rommert and K. Krochn,20021069961004Suciatmih and M. Rahmansyah,20138251257Pharmawati, M.,2009Grevillea spp. (Proteaceae).]]>131216Vinale, F., E.L. Ghisalberti, K. Sivasithamparam, R. Marra and A. Ritieni et al.,2009Trichoderma harzianum secondary metabolites during the interaction with different plant pathogens.]]>48705711Lou, J., L. Fu, Y. Peng and L. Zhou,20131858915935Hohenfeld, C.S., M.P. Santana, L.R.C. Junior, E.J. de Oliveira and S.A.S. de Oliveira,2018Neoscytalidium hyalinum and Fusarium solani associated with black and dry root rot diseases on cassava.]]>43422432Azliza, I.N., R. Hafizi, M. Nurhazrati and B. Salleh,2014Fusarium species isolated from wild grasses in Peninsular Malaysia.]]>438994Singh, G., A. Katoch, M. Razak, S. Kitchlu, A. Goswami and M. Katoch,2017Brugmansia aurea Lagerh.]]>2017Izzatinnisa, I., U. Utami and A. Mujahidin,2020Fusarium oxysporum in vitro]]>21825Aziza, N.L.,2019235462Mulaw, T.B., I.S. Druzhinina, C.P. Kubicek and L. Atanasova,2013Trichoderma spp. isolated from healthy Coffea arabica roots are capable of controlling coffee tracheomycosis.]]>5750766