Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2020.1572.1580YulianaT. HayatiF. CahyanaY. RialitaT. MardawatiE. HarahapB.M. SafitriR. 1220202312Background and Objective: The bacteriocin isolated from fermented buffalo milk from West Sumatra-Indonesia, called Dadih, can be considered as a natural and safe antimicrobial compound for food products. The objective of this research was to evaluate the antimicrobial activity of bacteriocin from Dadih and its effectiveness as a preservative in chicken meat. Materials and Methods: This study used experimental method followed by statistical analysis using 3 experiments with duplication including experiment of meat samples (0 and 10% bacteriocin), storage temperatures (7 and 26°C) and storage duration (0, 1, 2, 3, 4, 5, 6 days and 0, 6, 12 hrs). Each experiment consists of a bacteriocin test, antimicrobial activity assay, physicochemical measurement and storability. Results: From 10 LAB isolates successfully obtained from Dadih, two isolates with D7 code and D10 code had the highest antimicrobial activity, reaching 11.75 mm and 12 mm, respectively. The meat treated by 10% of bacteriocin gave the lower total microbial (3rd and 5th day) and total E. coli (5th day) at 7 and 26°C. The pH and water activity (aw) values of chicken meat with 10% of bacteriocin showed lower values at 7 and 26°C. The application of bacteriocin to chicken meat was able to inhibit the microbial growth that was still below standard for 3 days at 7°C and 6 hrs at 26°C. Conclusion: Based on research, lactic acid bacteria isolated from buffalo milk curd produced bacteriocin compound which has antimicrobial properties. This bacteriocin showed potential as a natural preservative for chicken meat by inhibiting the growth of pathogen microorganisms.]]>Khan, H., S. Flint and P.L. Yu,2010141110Khochamit, N., S. Siripornadulsil, P. Sukon and W. Siripornadulsil,2015Bacillus subtilis KKU213: Potential as a probiotic strain.]]>1703650Javaherzadeh, R., A.S.T. Bafroee and A. Kanjari,2020Polylophium involucratum essential oil incorporated poly lactic acid/ nanochitosan composite film on shelf life and sensory properties of chicken fillets at refrigeration temperature.]]>2020Garneau, S., N.I. Martin and J.C. Vederas,200284577592 Al Kassaa, I., R. Rafei, M. Moukhtar, M. Zaylaa and A. Gharsallaoui et al.,201954771779Acuña, L., G. Picariello, F. Sesma, R.D. Morero and A. Bellomio,201221219Zou, J., H. Jiang, H. Cheng, J. Fang and G. Huang,2018117781789Duan, X., S. Duan, Q. Wang, R. Ji, Y. Cao and J. Miao,2020Lactobacillus paracasei FX-6 on shelf life and microbial composition in chicken breast during refrigerated storage.]]>2020Kaya, H.I. and O. Simsek,20202020Kumariya, R., A.K. Garsa, Y.S. Rajput, S.K. Sood, N. Akhtar and S. Patel,2019128171177Cleveland, J., T.J. Montville, I.F. Nes and M.L. Chikindas,200171120Maragkoudakis, P.A., K.C. Mountzouris, D. Psyrras, S. Cremonese, J. Fischer, M.D. Cantor and E. Tsakalidou,2009Listeria monocytogenes and Salmonella enteritidis.]]>130219226Lv, X., H. Ma, M. Sun, Y. Lin, F. Bai, J. Li and B. Zhang,2018Lactobacillus plantarum from cutlassfish and its application as bio-preservative for the control of Pseudomonas fluorescens in fresh turbot (Scophthalmus maximus) fillets.]]>892231Fibri, D.L.N. and M.B. Frost,20191336169Sunaryanto, R. and B. Marwoto,201214228233Kaktcham, P.M., E.M.F. Kouam, M.L.T. Tientcheu, J.B. Temgoua, C. Wacher, F.Z. Ngoufack and M. De L. Perez-Chabela,2019Lactococcus lactis subsp. lactis 2MT isolated from freshwater Nile tilapia in Cameroon: Bacteriocin screening, characterization, and optimization in a low-cost medium.]]>107272279Wong, F.W.F., A.B. Ariff, S. Abbasiliasi and D.C. Stuckey,2017Pediococcus acidilactici Kp10 using surfactant precipitation.]]>232245252Swetwiwathana, A. and W. Visessanguan,2015109101105Hartmann, H.A., T. Wilke and R. Erdmann,2011Listeria monocytogenes in food.]]>146192199Macwana, S. and P.M. Muriana,201288713Favaro, L. and S.D. Todorov,20179444458Sun, T., Y. Qin, H. Xu, J. Xie, D. Hu, B. Xue and X. Hua,2017Penaeus vannamei.]]>105764768Braiek, O.B., S. Smaoui, K. Ennouri, S. Morandi, P. Cremonesi, K. Hani and T. Ghrairi,2019Enterococcus lactis strains and their potential on Listeria monocytogenes growth behaviour in stored chicken breast meats: Generalised linear mixed-effects approaches.]]>99244253Huff-Lonergan, E. and S.M. Lonergan,200571194204Arief, I.I., B.S.L. Jenie, T. Suryati, G. Ayuningtyas and A. Fuziawan,2012Lactobacillus plantarum 2C12 and its application on beef meatball as biopreservative.]]>379096Pateiro, M., F.C. Vargas, A.A.I.A. Chincha, A.S.S. Ana and I. Strozzi et al.,20181145563Badan, B.,2009Pages: 20Pages: 20