Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2019.283.290Portunus pelagicus (Linnaeus, 1758)]]>Efrizal Rusnam Suryati YolandaNofa SyaifulFerry Lismanto MardiahAinul 62019226Background and Objective: Spinach extract (Amaranthacea tricolor) is a very prospective feed raw materials to stimulate the molting and growth of crab female broodstock. The purpose of this study was to evaluate the quality of broodstock females, Portunus pelagicus (Linnaeus, 1758) at different levels of spinach extract based on physical, organoleptic and chemical tests. Materials and Methods: Four different treatments of spinach extract (0, 250, 500 and 750 ng g1 crab, respectively) were used in this study. The female crab samples were collected from coastal region of Padang, West Sumatera and placed randomly in four concrete tanks (200×100×100 cm). Each concrete tank consist of five units of plastic box (45.5×32.5×16.5 cm) with the maximum density was one crab per box. Results: The organoleptic test showed that formulated diets enriched with spinach extract (0, 250 and 500 ng g1 crab) had a smooth texture, pungent aroma and brown. Whereas from the physics test results obtained good water stability (rupture velocity ranged from 89.20-105.40 min and solids dispersion ranged from 4.97-7.17%), hardness (92.66-98.07%) and sinking velocity (3.88-5.88 cm sec1) (p<0.05). The results also showed that formulated diet enriched with spinach extract doses of 250-750 ng g1 crab gave a value of delicacy of feed (0.195-0.386 g crab1 weight/day) which was significantly different (p<0.05) with 0 ng g1 crab (0.445 g crab1 weight/day). The chemical test shows the moisture content were 11.60%, ash 9.31%, protein 44.38%, fat 7.64% and carbohydrate 14.46%. Conclusion: It was concluded that there was a linear relationship between dose of spinach extracts in formulated diet and rupture velocity and a quadratic relationship between dose of spinach extracts in formulated diet and solid disperse, hardness and sinking velocity and delicacy of feed.]]>FAO,2000pp: 713pp: 713Efrizal, A.A., M.S. Kamarudin, C.R. Saad and S.M.N. Amin,2015Portunus pelagicus (Linnaeus, 1758)) under laboratory conditions.]]>107791Hassan, A., T.N. Hai, A. Chatterji and M. Sukumaran, 2011Scylla serrata (Forsskal, 1775).]]>1416511654Romano, N., M.A. Safee, M. Ebrahimi and A. Arshad,2016Portunus pelagicus (Portunidae: Decapoda).]]>60112117Lockwood, A.P.M.,19671967Primavera, J.H.,19851985pp: 4764Watanabe, W.O., M.S. Alam, A.D. Ostrowski, F.A. Montgomery, J.E. Gabel, J.A. Morris Jr. and P.J. Seaton,2016Pagrus pagrus.]]>393107Millamena, O.M. and E.T. Quinitio,2000Scylla serrata.]]>1818190Aslamyah, S. and Y. Fujaya,20099133141Alava, V.R., A. Kanazawa, S.I. Teshima and S. Koshio,199359345351Efrizal and Rusnam,2017Portunus pelagicus (Linnaeus, 1758).]]>11715Fujaya, Y.,2011102428Aslamyah, S. and Y. Fujaya,201110815Aslamyah, S. and Y. Fujaya,2012Scylla sp.) melalui aplikasi pakan buatan berbahan dasar limbah pangan yang diperkaya dengan ekstrak bayam.]]>15170178Efrizal,2013Portunus pelagicus (Linnaeus, 1758), dengan manipulasi pakan alami dan buatan.]]>2013pp: 4551Efrizal, E.,2017Portunus pelagicus (Linnaeus, 1758) under laboratory conditions.]]>10217226Efrizal, E., I.J. Zakaria, R. Rusnam, S. Suryati and N. Yolanda,2018Portunus pelagicus (Linnaeus, 1758).]]>2018Badan Standarisasi Nasional,20062006Saade, E. and S. Aslamyah,2009Penaeus monodon fab. yang menggunakan berbagai jenis rumput laut sebagai bahan perekat.]]>19107115Efrizal,2015Portunus pelagicus (Linnaeus, 1758) under laboratory conditions.]]>9557563Balazs, G.H., E. Ross and C.C. Brooks,19732369377Badan Standarisasi Nasional,20022002Steel, R.G.D. and J.H. Torrie,19802nd Edn.,Pages: 633Pages: 633Aslamyah, S. and M.Y. Karim,2013Lumbricus sp.).]]>11124131Saenz de Rodriganez, M.A., B. Gander, M. Alaiz and F.J. Moyano,2011in vitro digestibility of commercial feeds used in weaning of marine fish.]]>17429440Murdinah, T., S. Suwarno and P. Sumpeno,1991702936Lim, C. and G. Cuzon,19947115127Mudjiman, A.,2009Makanan ikan.]]>2009Solomon, S.G., G.A. Ataguba and A. Abeje,2011Saccahromyces cerevisae).]]>115Lim, C. and G.W. Dominy,19911991pp: 149157ADCP.,1983pp: 97pp: 97Piedecausa, M.A., F. Aguado-Gimenez, B. Garcia-Garcia, G. Ballester and T. Telfer,2009Sparus aurata L. 1758) and seabass (Dicentrarchus labrax L. 1758).]]>4017031714Ayuba, V.O. and E.K. Iorkohol,20138248252Dale, N.,19976169173Mendez, A. and N. Dale,19987309312Wang, X. and C.M. Parsons,199877834841Oniam, V., L. Chuchit and W. Arkronrat,2012Portunus pelagicus) broodstock, fed with different feeds.]]>34381386Ding, L.Y., M. Jin, P. Sun, Y. Lu and H.N. Ma et al.,2017fabp and vitellogenin gene expression in the female swimming crab Portunus trituberculatus.]]>4745765Creswell, R.L.,19931993pp: 102-146pp: 102-146Alava, V.R. and F. Pascual,1987Penaeus monodon (Fabricius) juveniles.]]>61211217