Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2017.320.327Metarhizium anisopliae and Entomopathogenic Nematodes to Control Oryctes rhinoceros Larvae in the Rainy Season]]>IndriyantiDyah Rini WidiyaningrumPriyantini Haryuni SlametMuji MarettaYoris Adi 72017207Background and Objective: Metarhizium anisopliae (MET) and entomopathogenic nematodes (EPN) are microorganisms that attack the larvae of Oryctes rhinoceros. The effects of MET, EPN and the combination of both on the O. rhinoceros larvae were studied during the rainy season in Jepara Indonesia. This study aimed to determine the effectiveness of Metarhizium anisopliae and entomopathogenic nematodes to control Oryctes rhinoceros larvae in the rainy season. Materials and Methods: There were four level doses of MET, four level doses of EPN and four mixture of MET and EPN. The experiment used 72 containers that were placed in the garden with coconut palm shade. Five kilograms of organic soil that was mixed with biological control agents (MET, EPN and MET+EPN) and ten O. rhinoceros larvae 3rd instar were put in each other container. The data were analyzed by descriptive analysis. Results: Every larvae mortality was observed once a week and observations are for 8 weeks. The result showed that the larval mortality due to MET treatment occurred on 2nd-7th week. Meanwhile, the larval mortality due to EPN treatment took place on 2nd-8th weeks and the larval mortality due to MET+EPN treatment occurred on 1st-5th weeks. Conclusion: The combination of MET and EPN was simultaneously effective to control O. rhinoceros larvae than separate use of MET or EPN. Result of this study showed that using two agents of biocontrol was more effective, so that it can be beneficial for controlling O. rhinoceros larvae in the field.]]>BPTBUN.,2011Metarhizium anisopliae mushroom for controlling beetles (Oryctes rhinoceros) pest.]]>2011Bedford, G.O.,1986Oryctes rhinoceros) in the Southh Paciic by baculovirus.]]>15141147Prasad, G.S., V. Jayakumar, H.R. Ranganath and V.R. Bhagwat,2008Oryctes rhinoceros L. (Coleoptera: Scarabaeidae) by Oryctes baculovirus (Kerala isolate) in South Andaman, India.]]>27959964Manjeri, G., R. Muhamad and S.G. Tan,2014Oryctes rhinoceros beetles, an oil palm pest in Malaysia.]]>434293439Ansari, M.A., F.A. Shah and T.M. Butt,2008Metarhizium anisopliae as a new approach for black vine weevil, Otiorhynchus sulcatus, control.]]>129340347Sucipto,200924753Sevim, A., I. Demir, M. Hofte, R.A. Humber and Z. Demirbag,201055279297Gopal, M., A. Gupta and G.V. Thomas,2006Metarhizium anisopliae to check the breeding of insect pest, Oryctes rhinoceros L. in coconut leaf vermicomposting sites.]]>9718011806Gabarty, A., H.M. Salem, M.A. Fouda, A.A. Abas and A.A. Ibrahim,2014Beauveria bassiana and Metarhizium anisopliae in Agrotis ipsilon (Hufn.).]]>795100Soelistijono, R.,2015Rhizoctonia sp microryza on severity of Orchid Phalaenopsis amabilis's DSI disease against Fusarium sp.]]>7112119Indriyanti, D.R. and N.L. Muharromah,20168111118Acosta, E.W.,20112011Chaerani and B. Nurbaeti,2007Scirpophaga incertulas).]]>77179Subagiya,2005Steinernema carpocapsae against Crocidolomia binotalis pest in Tawangmangu.]]>73439Bunga, J.A.,2004Cylas formicarius (Coleoptera: Curculionidae).]]>2004Nderitu, J., M. Sila, G. Nyamasyo and M. Kasina,2009Cylas puncticollis (Coleoptera: Apionidae) under semi-field condition in Kenya.]]>6145154Climate-Data.ORG,20152015Indriyanti, D.R., B.K. Rani and P. Widiyaningrum,2015Metarhizium anisopliae M. fungus derived from infected larvae to Oryctes rhinoceros arvae mortality.]]>2015Erixon, M.H., S. Oemry and F. Zahara,2015Baculovirus oryctes and Metarhizium anisopliae (Metch.) Sorokin to control Brontispa longissima Gestro.]]>3124128Rani, B.K.,2015Metarhizium anisopliae to the of Oryctes rhinoceros larvae mortality.]]>2015Milner, R.J., P. Samson and R. Morton,2003Metarhizium anisopliae in sugarcane fields: Effect of isolate and formulation on persistence over 3.5 years.]]>13507516Tampubolon, D.Y., Y. Pangestiningsih, F. Zahra and F. Manik,2013Bacillus thuringiensis and Metarhizium anisopliae against Spodoptera litura Fabr (Lepidoptera: Noctuidae) mortality in laboratoratory.]]>1784791Mulyono,2007Metarhizium anisopliae fungi against Oryctes rhinoceros L. as a pests of coconut plants at various applications time.]]>2007Manurung, E.M., M.C. Tobing, L. Lubis and H. Priwiratama,2012Metarhizium anisopliae formulations against Oryctes rhinoceros L. larvae (Coleoptera: Scarabaeidae) di Insektarium.]]>14763Indriyanti, D.R.,2014Metarhizium anisopliae in Telogoweru Demak.]]>2014Hussein, K.A., M.A.A. Abdel-Rahman, A.Y. Abdel-Mallek, S.S. El-Maraghy and J.H. Joo,2010Beauveria bassiana and Metarhizium anisopliae in cultivated soil.]]>976747682Xiao, X., X. Wang, X. Zhang, E. Chen and J. Li,20155747760Hosang, M.L., A. Jelfina, C. Alouw and H. Novarianto,2004Brontispa longissima (Gestro) in Indonesia.]]>2004Hashim, N. and Y.B. Ibrahim,2003Paecilomyces fumosoroseus, Beauveria bassiana and Metarhizium anisopliae var. majus against Crocidolomia binotalis (Lepidoptera; Pyralidae).]]>26103108Imanadi, L.,2012Steinernema spp and Heterorhabditis spp).]]>2012Nugrohorini,2007Steinernema sp. nematoda (Tulungagung isolate) on plant sugar hama (Brassica juncea) at the laboratory.]]>1016