Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2017.100.107Candida guilliermondii]]>MohamedLagzouli KettaniYoussfi El AliAitounejjar MohamedElyachioui MohamedJadal 22017202Objective: Glucoamylase is among the most important enzymes in biotechnology. The present study aims to determine better conditions for growth and glucoamylase production by Candida guilliermondii and to reduce the overall cost of the medium using Box-Behnken design with one central point and response surface methodology. Methodology: Box-Behnken factorial design based on three levels was carried out to obtain optimal medium combination of five independent variables such as initial pH, soluble starch, CH4N2O, yeast extract and MgSO4. Forty one randomized mediums were incubated in flask on a rotary shaker at 105 rpm for 72 h at 30°C. Results: The production of biomass was found to be pH and starch dependent, maximum production when the starch concentration was 8 g L1 and the initial pH was 6, while maximum glucoamylase production was found at 6.5 of initial pH, 4 g L1 yeast extract and 6 g L1 starch, whereas yeast extract and urea were highly significant, but interacted negatively. Box-Behnken factorial design used for the analysis of treatment combinations gave a second-order polynomial regression model with R2 = 0.976 for Biomass and R2 = 0.981 for glucoamylase. Conclusion: The final biomass and glucoamylase activity obtained was very close to the calculated parameters according to the p-values (p<0.001), the predicted optimal parameters were confirmed and provides a basis for further studies in baking additives and in the valuation of starch waste products.]]>Kaur, P., N.K. Bhardwaj and J. Sharma,20162016pp: 133-156pp: 133-156Hu, Y., H. Qin, Z. Zhan, Y. Dun and Y. Zhou et al.,2016Saccharomyces boulardii production in solid-state fermentation with response surface methodology.]]>30173179Wu, W., S. Zhao, C. Chen, F. Ge, D. Liu and X. He,20142319411949Mosbah, H., I. Aissa, N. Hassad, D. Farh, A. Bakhrouf and S. Achour,2015Candida famata using factorial design.]]>63572580Oberoi, H.S., R. Rawat and B.S. Chadha,2014Aspergillus niger HN-2.]]>105119134Naili, B., M. Sahnoun, S. Bejar and R. Kammoun,2016Aspergillus oryzae S2 α-amylase production.]]>25185192Nachaiwieng, W., S. Lumyong, K. Yoshioka, T. Watanabe and C. Khanongnuch,2015Kluyveromyces marxianus CK8.]]>4543549Panda, T., T. Theodore and R.A. Kumar,2015Pages: 278Pages: 278Nielsen, S.,2014Pages: 602Pages: 602Feng, Y., X. Zhao, F. Lv, J. Zhang and B. Deng et al.,20152015Keera, A.A., F.E. Mouafi, T. Kahil, M. Fadel and A.A. Abedo,2014Aspergillus oryzae FK-923 under solid state fermentation.]]>3014471461Colla, L.M., A.L. Primaz, S. Benedetti, R.A. Loss and M. de Lima et al.,201647461467Kiran, E.U., A.P. Trzcinski and Y. Liu,2014198105Ramesh, V. and V.R. Murty,2014Humicola grisea MTCC 352.]]>2014Arnthong, J., B. Wanitchaploy, K. Sakai, J.J. Sanglier and V. Kitpreechavanich,2015973127316Ghosh, P., A. Das, S. Gayen, K.C. Mondal and U. Ghosh,2015Penicillium notatum NCIM 923 and kinetics study of the purified enzyme.]]>59179188Pervez, S., N.N. Siddiqui, A. Ansari, A. Aman and S.A.U. Qader,2015Aspergillus species for the production of starch-saccharifying amyloglucosidase.]]>6522872291Lakshmi, M.V.V.C. and P. Jyothi,201437076Wang, S., C. Lin, Y. Liu, Z. Shen, J. Jeyaseelan and W. Qin,2016Aspergillus niger WLB42 mutated by ethyl methanesulfonate treatment.]]>7110Lima-Orozco, R., I. van Daele, U. Alvarez-Hernandez and V. Fievez,2014in vitro assessment of their nutritive value.]]>152967980