Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2016.89.94Anacardium occidentale L.) In Albino Rat (Rattus norvegicus Berkenhout 1769)]]>Harlita SatutiN.H. Niken SagiMammed AstutiPudji 22016192Cashew plant (Anacardium occidentale L.) is a crop producing cashew nut shell that contain phenolic compounds such as lacquer oil (cashew nut shell liquid) which can be used for many studies. This study was conducted to determine the potency of acute toxicity (LD50) of cashew nut shell extract on female albino Wistar rats using Weil method. Twenty rats used in this study. The rats was divided into five groups, each consist of four rats after acclimatization. Each group was given the extract of cashew nut shell orally (force-fed). The amount of cashew nut shell extract that were given to group I, II, III and IV were 2.5, 25, 250 and 2,500 mg kg1 b.wt., respectively, while group V were given 0.5% sodium carboxyl methyl cellulose (CMCNa) solution. Clinical symptoms were observed 24 h after the administration of extract include behavioral changes i.e., licking, scratching, twitching, tremors, wrihing, reactivity to stimuli, cerebral and spinal reflexes, secretions, breath, skin, hair and death. Probit analysis using Weil method was used as an effective dose. The results showed that the potency for acute toxicity (LD50) of cashew nut shell extract was 2,018 mg kg1 which classified as moderately toxic category. The administration of extract also causes behavioral changes in animal including passivity and mucus secretion. All doses of the extract did not affect the development body weight and the weight of organs (spleen, liver, heart, kidneys and lungs) in female rats.]]>Arcanjo, D.D.R., A.C.M. Albuquerque, B. Melo-Neto, L.C.L.R. Santana, M.G.F. Medeiros and A.M.G.L. Cito,2012Artemia salina Leach of medicinal plants used in Brazilian Northeastern folk medicine.]]>72505509Donatus, I.A.,20012001Harmita and M. Radji,2006Harlita, H.,2004Anacardium occidentale L) as molluscicides to inhibit reproduction Keong Mas (Pomacea sp).]]>166Hemshekhar, M., M.S. Santhosh, K. Kemparaju and K.S. Girish,2012110122132Hodgson, E. and P.E. Levi,20002nd Edn.,El Hilaly, J., Z.H. Israili and B. Lyoussi,2004Ajuga iva in experimental animals.]]>914350Kannan, V.R., C.S. Sumathi, V. Balasubramanian and N. Ramesh,2009Anacardium occidentale L.) nuts.]]>2253257Kubo, I., H. Muroi, M. Himejima, Y. Yamagiwa and H. Mera et al.,19934110161019Leite, A.S., A.F. Dantas, G.L.S. Oliveira, A.I. Gomes Jr. and S.G. de Lima et al.,2015Allium cepa and Artemia salina bioassays.]]>2015Madamba, L.S.P., R.O. Quimaldo, M.E. Flaveier and M.S. Villau,1991Anacardium occidentale) nut oil.]]>74511511Lu, F.C.,19912nd Edn.,Michael, B., B. Yano, R.S. Sellers, R. Perry and D. Morton et al.,200735742750Mir, A.H., M. Sexena and M.Y. Malla,2013Tridex procumbens in sprague dawley's rats as per OECD guidelines 423.]]>31620Murray, R.S.,1992Ping, K.Y., I. Darah, Y. Chen, S. Sreeramanan and S. Sasidharan,2013Euphorbia hirta L. methanol extract in rats.]]>2013Roopashree, T.S., R. Dang, R.H. Shobha Rani and C. Narendra,2009Calendula officinalis, Momordica charantia, Cassia tora and Azadirachta indica seed oil.]]>337483Simpen, I.N.,2008Anacardium occidentale L) dan Kajian Beberapa Sifat Fisiko-Kimianya.]]>27176Steenis,1997Sornprom, N.,20072007Sullivan, J.T., C.S. Richards, H.A. Lloyd and G. Krishna,198244175177Thompson, E.B.,1985Pages: 164Pages: 164Yance, D.R. and B. Tabachnik,20072007Zbinden, G. and M. Flury-Roversi,198150-test for the toxicological evaluation of chemical substances.]]>477799