Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2016.43.48MaalhaghMehrnoosh JahromiAbdolreza Sotoodeh YusefiAlireza RazeghiAli ZabetiyanHassan KaramiMohammad Yasin MadaniAbdol Hossein 12016191Stress has inhibitory effect on HPG axis through increasing cortisol serum level. In this study, the effect of acute prepubertal stress on kisspeptin, which plays essential role in puberty achievement is assessed. To do this experimental study thirty immature healthy male wistar rats of 4 weeks old and without any symptoms of puberty were selected randomly. These rats were divided into three groups, randomly. Two groups were chosen as control and pretest and one as stress (test) group. Immobilization stress was applied for 10 days and serum level of cortisol, testosterone and kisspeptin were measured. Primary and secondary spermatocyte and sertoli cell evaluated and compared among groups. Mean serum level of kisspeptin in pretest group, control group and stress (test) group were 0.0381±0.0079, 91.0500±4.87430 and 15.2156±3.88135 pg mL1 respectively. Serum level of kisspeptin had significant differences between three groups (p<0.001). Acute prepubertal immobilization stress led to decrease in serum level of kisspeptin and testosterone in stress (test) group compared to control groups. Also stress caused a significant decrease in the numbers of secondary spermatocytes of the test group.]]>Almeida, S.A., J.A. Anselmo-Franci, A.A. Rosa-e-Silva and T.L.L. Carvalho,199883701704Aziz, N.M., M.M. Ragy and M.F. Gayyed,201324255262Barnea, E.R. and J. Tal,199181523Bianco, S.D.C.,20122012Blum, W.F., P. Englaro, S. Hanitsch, A. Juul and N.T. Hertel et al.,199782290429010Castellano, J.M., A.H. Bentsen, M. Romero, R. Pineda and F. Ruiz-Pino et al.,2010299E54E61Huhman, K.L., T.O. Moore, C.F. Ferris, E.H. Mougey and J.L. Meyerhoff,199125206216Iwasa, T., T. Matsuzaki, M. Murakami, R. Kinouchi and R. Ogata et al.,200927345349Jameel, M.K., A.R. Joshi, J. Dawane, M. Padwal, A.R. Joshi, V.A. Pandit and R.R. Melinkeri,20148181183Kanasaki, H., I.N. Purwana, A. Oride, T. Mijiddorj, U. Sukhbaatar and K. Miyazaki,201329583587Kinsey‐Jones, J.S., X.F. Li, A.M.I. Knox, E.S. Wilkinson and X.L. Zhu et al.,2009Kisspeptin and its receptor, Kiss1r, mRNA expression is associated with stress-induced suppression of luteinising hormone secretion in the female rat.]]>212029Majzoub, J.A. and K.P. Karalis,1999180S242S246Maric, D., T. Kostic and R. Kovacevic,199658351355Mogford, J.T., C.N. Jayasena and W.S. Dhillo,20149251261Morelli, A., M. Marini, R. Mancina, M. Luconi and L. Vignozzi et al.,2008510971113Navarro, V.M., J.M. Castellano, D. Garcia-Galiano and M. Tena-Sempere,200781120Nirupama, M., M. Devaki, R. Nirupama and H.N. Yajurvedi,2013695968Pineda, R., D. Garcia-Galiano, A. Roseweir, M. Romero and M.A. Sanchez-Garrido et al.,2010151722730Rao, Y.S., N.N. Mott and T.R. Pak,201139220228Rhie, Y.J.,2013185559Robb, G.W., R.P. Amann and G.J. Killian,197854103107Sawyer, I., S.J. Smillie, J.V. Bodkin, E. Fernandes, K.T. O'Byrne and S.D. Brain,20112011Shibata, M., R.L. Friedman, S. Ramaswamy and T.M. Plant,2007KiSS-1 expression is involved in the negative feedback action of testosterone to regulate luteinising hormone secretion in the adult male rhesus monkey (Macaca mulatta).]]>19432438Skorupskaite, K., J.T. George and R.A. Anderson,201420485500Smith, J.T., D.K. Clifton and R.A. Steiner,2006131623630Zhang, M.J., Z.L. Gong, D. Han, X. Gao, Q. Tan and Y. Xu,2013647466470