Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2014.999.1006Camellia sasanqua as Antioxidant Agents]]>SukitoAgus TachibanaSanro 82014178Two antioxidant active compounds were isolated from the methanol
extract of Camellia sasanqua using various in vitro assays: 1,1-diphenyl-2-picrylhydrazyl
(DPPH), β-carotene bleaching and reducing power assays. The ethyl acetate
(EtOAc) fraction of the methanol extract had the highest DPPH radical-scavenging
activity with an Inhibition Concentration (IC50) value of 18.3±1.63
μg mL-1. Sephadex LH-20 column chromatography was used to separate
the EtOAc fraction into eight fractions (F1-F8). Antioxidant activity was significantly
higher in fraction 5 with an IC50 value 14.61±0.02 μg
mL-1. Fraction 5 was further separated by HPLC preparative with Capcellpak
C18 MG followed by the Cosmosil 5C18-AR-II column, using
a guided DPPH radical-scavenging assay. The compounds isolated were identified
as: Hyperoside (1) and isoquercitrin (2) after recrystallization from ethanol,
based on Mass Spectrum (MS) and Nuclear Magnetic Resonance (NMR) analyses. Their
DPPH radical-scavenging activities based on the 50% scavenging concentration
decreased in the following order: Isoquercitrin (21.6 mM)>hyperoside (27.5
mM). The antioxidant activities of hyperoside and isoquercitrin were 67.52±0.64
and 64.33±0.51%, respectively, in the β-carotene bleaching assay.
These compounds were found to have good reducing powers (OD value: 2.5-3.8)
at concentrations of 50-140 μg mL-1, using the potassium ferricyanide
reduction method. Although, these compounds are well-known, hyperoside (1) was
isolated from this herb for the first time.]]>Akihisa, T., K. Yasukawa, Y. Kimura, S. Yamanouchi and T. Tamura,199848301305Cao, G., E. Sofic and R.L. Prior,199722749760Cho, J.Y., S.H. Ji, J.H. Moon, K.H. Lee, K.H. Jung and K.H. Park,2008Camellia japonica.]]>1710601065Das, D., D. Bandyopadhyay, M. Bhattacharjee and R.K. Banerjee,199723818Devasagayam, T.P.A., J.C. Tilak, K.K. Boloor, K.S. Sane, S.S. Ghaskadbi and R.D. Lele,200452794804Fishman, G.M. and V.A. Bandyukova,1991Camellia sasanqua.]]>27371371Gazzani, G., A. Papetti, G. Massolini and M. Daglia,19984641184122Heinecke, J.W.,2003911216Jayaprakasha, G.K., R.P. Singh and K.K. Sakariah,2001Vitis vinifera) extracts on peroxidation models in vitro.]]>73285290Kulisic, T., A. Radonic, V. Katalinic and M. Milos,200485633640Koleva, I.I., T.A. van Beek, J.P.H. Linssen, A. de Groot and L.N. Evstatieva,200213817Lee, H.H., J.Y. Cho, J.H. Moon and K.H. Park,2011Camellia japonica flowers.]]>52270277Lelono, R.A.A. and S. Tachibana,2013Eugenia polyantha.]]>16812818Matsuda, H., S. Nakamura, K. Fujimoto, R. Moriuchi and Y. Kimura et al.,2010Camellia sasanqua.]]>5816171621Meister, A.,199426993979400Muramatsu, H., K. Kogawa, M. Tanaka, K. Okumura and Y. Nishihori et al.,19955562106214Onodera, K., K. Hanashiro and T. Yasumoto,2006Camellia japonica.]]>7019951998Oyaizu, M.,198644307315Pekkarinen, S.S., H. Stockmann, K. Schwarz, I.M. Heinonen and A.I. Hopia,19994730363043Perron, N.R. and J.L. Brumaghim,20095375100Pietta, P.G.,20006310351042Rosen, P., T. Ballhausen, W. Bloch and K. Addicks,19953811571168Sawa, T., M. Nakao, T. Akaike, K. Ono and H. Maeda,199947397402Van Acker, S.A.B.E., D.J. van den Berg, M.N.J.L. Tromp, D.H. Grifioen, W.P. van Bennekom, W.J.F. van der Vijgh and A. Bast,199620331342Seeram, N.P. and M.G. Nair,20025053085312Steinberg, D., S. Parthasarathy, T.E. Crew, J.C. Khoo and J.L. Witztum,1989320915924Yamaguchi, T., H. Takamura, T. Matoba and J. Terao,19986212011204Yen, G.C. and H.Y. Chen,1995432732Rice-Evans, C.A., N.J. Miller and G. Paganga,199620933956