Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2014.891.897In situ Hybridization: A Pilot Study]]>AnnoozAaedah F. MelconianAlice K. Abdul-MajeedBan A. JawadAli M. 72014177P53 as a tumor suppressor gene plays a major role in cancer
development, it is essential for cell growth regulation and apoptosis. The deletion
of p53 is known to be associated with aggressive diseases in several hematological
malignancies. The evidence indicated that p53 deletions can be acquired as a
result of chemotherapy. Therefore, a follow-up study for p53 gene deletion by
fluorescence in situ hybridization technique (FISH) was carried out for
the patients group who affected with different hematological malignancies before
and after chemotherapy. The main goals from screening of p53 deletion were to
assess the correlation between p53 deletion and chemotherapy resistance, overall
median survival and chromosomal abnormalities. It is concluded from the present
study that p53 deletion has a cardinal effect on the clinical outcome (chemotherapy
resistance, overall median survival) and outcome of chromosomal abnormalities
(quality and quantity of chromosomal abnormalities) of the patients who were
affected with hematological malignancies before and after chemotherapy.]]>Hollstein, M., D. Sidransky, B. Vogelstein and C.C. Harris,19912534953Shimamura, A. and D.E. Fisher,19962435440Hahn, W.C., C.M. Counter, A.S. Lundberg, R.L. Beijersbergen, M.W. Brooks and R.A. Weinberg,1999400464468Peller, S. and V. Rotter,200321277284Wattel, E., C. Preudhomme, B. Hecquet, M. Vanrumbeke and B. Quesnel et al.,19948431483157Ferbeyre, G., E. de Stanchina, E. Querido, N. Baptiste, C. Prives and S.W. Lowe,20001420152027Ferbeyre, G., E. de Stanchina, A.W. Lin, E. Querido, M.E. McCurrach, G.J. Hannon and S.W. Lowe,20022234973508Gaumont-Leclerc, M.F., U.K. Mukhopadhyay, S. Goumard and G. Ferbeyre,20042794680246809Giaccia, A.J. and M.B. Kastan,19981229732983Bonnefoi, H., A. Ducraux, S. Movarekhi, M.F. Pelte, S. Bongard, E. Lurati and R. Iggo,200286750755Venkat, P.C.,20032003Czepulkowski, B.H., B. Bllatt and D.E. Rooney,19921992pp: 1-24 pp: 1-24 Shaffer, L., M. Slovak and L. Cambell,2009Al-Achkar, W., A. Wafa and M.S. Nweder,200726411415Daniel, W.W.,1985pp: 363-394pp: 363-394Daniel, W.W.,1985pp: 275-321pp: 275-321Chang, H., C. Qi, Q.L. Yi, D. Reece and A.K. Stewart,2005105358360Kremer, M., M. Spitzer, S. Mandl-Weber, K. Stecker and B. Schmidt et al.,200383107114Rubin, E.H. and W.N. Hait,20032003pp: 2282-2298pp: 2282-2298Olivier, M.,20082008Christiansen, D.H., M.K. Andersen and J.P. Biergaard,20011914051413Keshelava, N., J.J. Zuo, P. Chen, S.N. Waidyaratne and M.C. Luna et al.,20016161856193Feinstein, E., G. Cimino, R.P. Gale, G. Alimena and R. Berthier et al.,19918862936297Di Bacco, A., K. Keeshan, S.L. McKenna and T.G. Cotter,20005405415Honda, H., T. Ushijima, K. Wakazono, H. Oda and Y. Tanaka et al.,20009511441150Gandhi, V.,200748219220Drach, J., J. Ackermann, E. Fritz, E. Kromer and R. Schuster et al.,199892802809Lozanski, G., N.A. Heerema, I.W. Flinn, L. Smith and J. Harbison et al.,200410332783281Tweddle, D.A., A.J. Malcolm, N. Bown, A.D. Pearson and J. Lunec,200161813Connor, M. and M. Ferguson-Smith,20042004pp: 162-178pp: 162-178Moorman, A.V., C.J. Harrison, G.A.N. Buck, S.M. Richards and L.M. Secker-Walker et al.,200710931893197Yang, C.P., J.H. Wu, I.J. Hung and T.H. Jaing,200099281289Grimwade, D., H. Walker, F. Oliver, K. Wheatley and C. Harrison et al.,19989223222333Stefanova, M., J. Dierlamm, L. Michaux, P. Leberecht, D. Seeger, K. Hinz and D.K. Hossfeld,2000119158161Fassas, A.B.T., T. Spencer, J. Sawyer, M. Zangari and C.K. Lee et al.,200211810411047Johansson, B., T. Fioretos and F. Mitelman,20021077694Bertrand, P., D. Rouillard, A. Boulet, C. Levalois, T. Soussi and B.S. Lopez,19971411171122Paulson, T.G., A. Almasan, L.L. Brody and G.M. Wahl,19981830893100Kojima, K., M. Konopleva, I.J. Samudio, M. Shikami and M. Cabreira-Hansen et al.,200510631503159