Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2014.829.835Ficus sycomorus L. (Moraceae)]]>Ramde-TiendrebeogoAlphonsine TibiriAndre Moussa Ouedraogo Sylvin Ouedraogo Odile Germaine Nacoulma Innocent Pierre Guissou 62014176The leaves of Ficus sycomorus are used in Burkina Faso
folk medicine for the treatment of sickle cell disease. The present comparative
study of crude extracts of leaves (decoction, macerated extract and a 95% ethanol
extract) was performed with the aim to assess the efficiency of this traditional
use and to determine the most active of the three extracts. Antisickling activity
was assessed by the Emmels test. Vasorelaxant effect on rat aortic rings precontracted
by phenylephrine with and without N-nitro-L-arginine methyl ester chloride (L-NAME)
was also evaluated. The 95% ethanol extract (20 mg mL-1) showed the
most antisickling activity on sickle erythrocytes, by inhibiting completely
sickling of double heterozygote SC cells in 60 min and that of homozygote SS
cells in 90 min. On the aorta this extract exhibited a significant (p<0.05)
vasorelaxant activity, better than that of the other extracts, with an IC50
value of 6.86±0.13 mg mL-1 against 18.78±0.38 and 28.56±1.27 mg mL-1,
respectively for the macerated extract and the decoction. When the aortic rings
were pretreated with L-NAME, only the ethanolic extract conserved its vasorelaxant
activity, up to 73% of relaxation. The acute toxicity of the decoction, assessed
by intraperitoneal route and using the Litchfield and Wilcoxon method, led to
an LD50 value of 1553.61 mg kg-1 b.wt. This places the
drug among those with low toxicity according to the WHO scale. These results
confirm those previously obtained and provide a scientific basis supporting
the use of this plant in folk medicine against sickle cell disease. They indicate
the importance of Ficus sycomorus in the research of new antisickling
molecules.]]>Abdel-Hameed, E.S.S.,2009Ficus species leaf samples.]]>11412711277Andriambeloson, E., A.L. Kleschyov, B. Muller, A. Beretz, J.C. Stoclet and R. Andriantsitohaina,199712010531058Arbonnier, M.,20001st Edn.,pp: 541pp: 541Charbonney, E., M. Terrettaz, N. Vuilleumier and J.F. Lambert,2006228522857Diagne, I., N.D. Diagne-Gueye, H. Signate-Sy, B. Camara and P. Lopez-Sall et al.,200363513520Furchgott, R.F. and J.V. Zawadzki,1980288373376Girot, R. and P. Begue,2003pp: 321pp: 321Guissou, I.P., M. Sawadogo, A. Sawadogo and A. Ouattara,199512938Hirt, H.M. and B. M'Pia,1995Pages: 118Pages: 118Litchfield, Jr. J.T. and F. Wilcoxon,19499699113Modell, B. and M. Darlison,200886480487Mpiana, P.T., V. Mudogo, D.S. Tshibangu, E.K. Kitwa and A.B. Kanangila et al.,2008Bombax pentadrum, Ficus capensis and Ziziphus mucronata: Photodegradation effect.]]>120413418Mpiana, P.T., D.S.T. Tshibangu, O.M. Shetonde and K.N. Ngbolua,2007In vitro antidrepanocytary actvity (anti-sickle cell anemia) of some congolese plants.]]>14192195Nongonierma, R.B., G.Y. Sy, L. Ndiaye, D. Thiam, I. Samb and A. Samb,2005Ficus gnagphalocarpa stem bark acetone extract].]]>18736Ramde-Tiendrebeogo, A., A. Tibiri, A. Hilou, M. Lompo, H. Millogo-Kone, O.G. Nacoulma and I.P. Guissou,2012Ficus sur Forssk. and Ficus sycomorus L. (Moraceae): Potential for sickle cell disease treatment in Burkina Faso.]]>6328336Rees, D.C., T.N. Williams and M.T. Gladwin,201037620182031Reiter, C.D., X. Wang, J.E. Tanus-Santos, N. Hogg, R.O. Cannon, A.N. Schechter and M.T. Gladwin,2002813831389Sandabe, U.K., P.A. Onyeyili and G.A. Chibuzo,2006Ficus sycomorus L. (Moraceae) stem bark on muscular activity in laboratory animals.]]>103481483Sess, E.D., M.A. Carbonneau, M. Meite, E. Peuchant and M.F. Dumont et al.,199891238241Weatherall, D., O. Akinyanju, S. Fucharoen, N. Olivieri and P. Musgrove,20062006pp: 663-680pp: 663-680WHO.,2010Pages: 78Pages: 78