Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2014.667.674Penicillium sp.) of Annona squamosa L.]]>YuniantoPrasetyawan RusmanYudi SaepudinEndang SuwarsoWahyudi Priyono SumaryonoWahono 52014175Several endophytic fungal strains from Srikaya plants (Annona squamosa L.) have been isolated and one of them was identified as Penicillium sp. Penicillium has been proven as an established source for a wide array of unique bioactive secondary metabolites that exhibit a variety of biological activities. The aim of this study is isolation of secondary metabolite from Penicillium, an endophytic of A. squamosa L. Penicillium sp. from endophytic of A. squamosa L. was fermented in Wicherham media. The whole extract from both liquid media and mycelium was partitioned by ethyl acetate and evaporated to obtain crude ethyl acetate extract. The ethyl acetate extract was then brokedown using column chromatography with silica as stationary phase and mixture of ethyl acetate/methanol (98%:2% ) as mobile phase and then was separated by sephadex column. Structure elucidation of isolated compounds were mainly done by analysis of one and two dimensional NMR (Nuclear Magnetic Resonance) data and supported by HPLC (High performance Liquid Chromatography) and MS-TOF (Mass Spectrometer-Time of Flight). Isolated secondary metabolites were tested using in vitro assays for anticancer and antimicrobial activity. For anticancer activity, the metabolites were tested against breast cancer cells (MCF-7) using MTT assay, while for antimicrobial activity was performed using disk diffusion assays. From these physical, chemical and spectral evidences that the secondary metabolites were confirmed as Chrysogine and Meleagrine. Chrysogine and Meleagrine have no activity as anticancer and antimicrobial.]]>Bhatnagar, I. and S.K. Kim,2010826732701CCRC,20082008Ebel, R., P. Proksch and W.E.G. Muller,20062006pp: 73-144pp: 73-144Effendi, H.,20042004Frisvad, J.C., J. Smedsgaard, T.O. Larsen and R.A. Samson,2004Penicillium subgenus Penicillium.]]>49201241Houbraken, J., J.C. Frisvad and R.A. Samson,2011Penicillium chrysogenum but P. Rubens.]]>28795Joseph, B. and R.M. Priya,20111291309Kawai, K., K. Nozawa, S. Nakajima and Y. Iitaka,1984329498Keller, N.P., G. Tunner and J.W. Bennet,20053937947Kettering, M., O. Stener and T. Anke,200459816823Kim, H.Y., H.M. Park and C.H. Lee,2012Penicillium species (P. Echinulatum, P.expansum, P.solitum and P.oxalicum) and its corelation with antioxcidant activity.]]>90327335Kumala, S., R. Utji, P. Sudarmono and L.B.S. Kardono,2006Brucea javanica L. (Merr.) and cytotoxic evaluation of their n-butanol extract from fermentation broth.]]>9825832Leatemia, J.A. and M.B. Isman,2004Annona spp., Lansium domesticum and Sandoricum koetjape against lepidopteran larvae.]]>323037Liaw, C.C., Y.L. Yang, M. Chen, F.R. Chang, S.L. Chen, S.H. Wu and Y.C. Wu,2008Annona squamosa as cytotoxic agents and calcium ion chelators.]]>71764771Meng, L., P. Sun, H. Tang, L. Li and S. Draeger et al.,2011Penicillium chrysogenum, a new source of hypocrellins.]]>39163165McLaughlin, J.l. and C.H. David,20012001Nielsen, K.F.,200339103117Oliveira, C.M., L.O. Regasini, G.H. Silva, L.H. Pfenning and M.C.M. Young et al.,2011Xylaria sp. And Penicillium sp., endophytic fungi associated with Piper aduncum and Alibertia macrophylla.]]>49396Prijono, D., M.S. Gani and E. Syahputra,1997Crocidolomia binotalis Zeller (Lepidoptera: Pyralidae).]]>916Rusman, Y.,2006Samson, R.A., J. Houbraken, U. Thrane, J.C. Frisvad and B. Andersen,2010Pages: 390Pages: 390Shabab, M., Y.P. Mhaindarkar, S. Kumar, M.I. Khan and S.H. Bhosale,2011penicillium sp. SDBF1 and partial characterization of its cysteine protease inhibitor.]]>2135142Sharif, S., G.S. Denisov, M.D. Toney and H.H. Limbach,200712962136327Swanson, S.M. and J.M. Pezzuto,19901990pp: 273-297 pp: 273-297 Tan, R.X. and W.X. Zou,200118448459Vinokurova, N.G., L.V. Boichenko and M.U. Arinbasarov,200339457460Yunianto, P., S. Rosmalawati, I. Rachmawati, W.P. Suwarso and W. Sumaryono,2012Annona squamosa) having potential secondary metabolites as anti-breast cancer activity.]]>62329