Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2014.182.189Retama raetam in Southern Tunisia]]>AbdellaouiRaoudha YahyaouiFaouzia NeffatiMohamed 22014172Retama raetam is a stem-assimilating, C3, evergreen,
medicinal plant species, desert legume common to arid ecosystems around the
Mediterranean basin. This study addresses the genetic diversity and relationship
among and within three populations collected from different habitats in southern
Tunisia by Random Amplified Polymorphic DNA (RAPD). Estimates of the percentage
of polymorphic bands, Shannons diversity information index and Neis
gene diversity index were determined. Results showed that population from the
Island Djerba has the lowest Neis gene diversity; this also was for Shannon
diversity index. An analysis of molecular variance indicated that the majority
of variation existed within populations (68%) and that there was significant
differentiation among populations (ΦPT = 0.316, p<0.001). Genetic distance
(ΦPT based values) between pair-wise populations ranged from 0.098 to 0.505
and the differentiation between pair-wise populations was significant when individual
pairs of populations were compared. Based on the coefficient of gene differentiation
(Gst), gene flow (Nm) was estimated and was found to vary from 0.490 to 4.609
between pair-wise populations and 1.42 among populations. The results of UPGMA
cluster analysis and PCoA analysis indicated that most variation occurred within
populations and that genetic differentiation had happened between populations.
These findings are important for a better understanding of the adaptive strategy
of R. raetam in southern Tunisia and will be useful for conservation
managers to work out an effective strategy to protect this important species.]]>Abdellaoui, R., H. Gouja, A. Sayah and M. Neffati,2011Calligonum species.]]>49695703Al-Tubuly, R.A., A. Auzi, A.A. Al-Etri-Endi, N. Lutfun and S.D. Sarker,2011Retama reatam (Forssk.) Webb and Berthel. (Fabaceae) on the central nervous system in experimental animals.]]>6310151021Ayala, F.J. and J.A. Keger,19842nd Edn.,Pages: 923Pages: 923Burits, M. and F. Bucar,2000Nigella sativa essential oil.]]>14323328CRC Weed Management Guide,2003Retama raetam.]]>2003Dawson, I.K., A.J. Simons, R. Waugh and W. Powell,1995Gliricidia sepium revealed by PCR-based assays.]]>741018Gustafson, D.J., J. Gibson and D.L. Nickrent,1999Andropogon gerardii Vitman) populations from Arkansas Grand Prairie.]]>816931701Helm, A., T. Oja, L. Saar, K. Takkis, T. Talve and M. Partel,20099713291336Karimi, H.R., S. Kafkas, Z. Zamani, A. Ebadi and R. Fatahi,2009Pistacia species using AFLP markers.]]>2792128Kassem, M., S.A. Mosharrafa, N.AM. Saleh and S.M. Abdel-Wahab,2000Retama raetam.]]>71649654Kate, K.T. and S.A. Laird,1999Pages: 398Pages: 398Larsen, H.O. and C.S. Olsen,20071616791697Lewis, P.O. and D.J. Crawford,1995Pleistocene refugium endemics exhibit greater allozyme diversity than wide spread congeners in the genus Polygonella (Polygonaceae).]]>82141149Mantel, N.,196727209220McDermott, J.M. and B.A. McDonald,199331353373Messaoud, C., M. Afif, A. Boulila, M.N. Rejeb and M. Boussaid,2007Tunisian Myrtus communis L. (Myrtaceae) populations assessed by isozymes and RAPDs.]]>64845853Mittler, R., E. Merquiol, E. Hallak-Herr, S. Rachmilevitch, A. Kaplan and M. Cohen,2001Retama raetam.]]>25407416Nei, M.,19737033213323Orloci, L.,1975Ouji, A., M. Jose Suso, M. Rouaissi, R. Abdellaoui and M. El Gazzah,2011Vicia faba L.) populations revealed by isozyme markers.]]>333138Peakall, R. and P.E. Smouse,20066288295Pluess, A.R. and J. Stocklin,20049120132021Qian, W. and S. Ge,200128244255Rohlf, F.C.,1994Schaal, B.A., W.J. Leverich and S.H. Rogstad,19911991pp: 123-134pp: 123-134Schippmann, U., D.J. Leaman and A.B. Cunningham,20022002Solouki, M., H. Mehdikhani, H. Zeinali and A.A. Emamjomeh,2008Matricaria chamomilla) based on morphological traits and molecular markers.]]>117281287Spooner, A., J. Carpenter, G. Smith and K. Spence,2008Retama raetam (Forssk.) wbb.]]>2008Staub, J.E., Y. Danin-Poleg, G. Fazio, T. Horejsi, N. Reis and N. Katzir,2000Cucumis melo L. using random amplified polymorphic DNA and simple sequence repeat markers.]]>115225241Sui, X.Q., K. Wang, S.H. Zheng, L. Li and S.Z. An,2009Stipa krylovii Roshev (Stipa L.) in agro-pastoral ecotone in Northern China.]]>834313439Wang, J.L., Y.B. Gao, N.X. Zhao, A.Z. Ren and W.B. Ruan et al.,2006Stipa krylovii Roshev. in Inner Mongolia steppe.]]>472335Williams, J.G.K., A.R. Kubelik, K.J. Livak, J.A. Rafalski and S.V. Tingey,19901865316535Wright, S.,19311697159Yeh, F.C., R. Yang and T. Boyle,1999Version 1.31,Zhang, D.Q., L.M. Gao and Y.P. Yang,2010Fritillaria cirrhosa (Liliaceae) in southwest China and implications for its conservation.]]>38236242Zhao, N.X., Y.B. Gao, J.L. Wang and A.Z. Ren,2008Stipa grandis P. Smirn, a dominant species in the typical steppe of northern China.]]>36110Zheng, W., L. Wang, L. Meng and J. Liu,2008Anisodus tanguticus (Solanaceae), an alpine perennial endemic to the Qinghai-Tibetan Plateau.]]>132123129Dyer, A.R. and K.J. Rice,1997112333339Qiu, Y.X., X.W. Zhou, C.X. Fu and Y.S.G. Chan,2005Dysosma versipellis (Berberidaceae).]]>466169