Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2014.1209.1218Phoenix dactylifera L.) in vitro]]>AldaejMohammed I. AlturkiSaleh M. ShehataWael F. GhazzawyHesham S. 1220141712Antioxidants present in dates are necessary for all physiological processes of humans and animals. In Saudi Arabia date palm is a national fruit tree, produces millions of tons of dates for consumption and is considered a major source of antioxidants. The main aim of this study was to determine the role of potassium nitrate (KNO3) in the formation of antioxidants from explants collected from date palm cultivars in Al-Ahsa Oasis, Kingdom of Saudi Arabia (KSA) and to monitor the extent of its effect on growth and development of cells during callus formation stage via somatic embryogenesis. The results showed that full concentration of KNO3 was the best for callus formation in general. While, the half concentration of KNO3 played an important role for stimulating the explants to form phenolic compounds and the browning emergence for all the cultivars under investigation. On the other hand, the chemical analysis for measuring the phenolic compounds in the explants showed that all the explants formed antioxidants but with varying degrees. The highest mean of phenolic contents was found in those explants cultured with the half concentration of KNO3 for Shishi cv 2.053±0.010a mg g-1 and antioxidant activity by ABTS Inhibition and UM Trolox was 80.694±0.439 and 801.575±2.391, respectively.]]>Abo-El-Nil, M.M.,19861986pp: 5965Abul-Soad, A.A., N.R. El-Sherbini and E.E. Bakr,2004Phoenix dactylifera L. cv. Zaghloul).]]>2004pp: 139163Al-Ibresam, O.T. and U.N. Al-Meer,2008Phoenix dactylifera L.) by in vitro.]]>7116Al-Kharyi, J.M. and K.W. Al-Marri,1997332226Al-Khateeb, A.A.,2008in vitro bud formation of date palm (Phoenix dactylifera L.) cv. Khanezi by different carbon sources.]]>9965506555Al-Khayri, J.M.,2001Phoenix dactylifera L.).]]>37453456Al-Marri, K.W.,19951995Al-Redhaiman, K.N.,200422532Al-Redhaiman, K.N.,20042004Al-Turki, S., M.A. Shahba and C. Stushnoff,2010Phoenix dactylifera L.) fruits as affected by cultivar and location.]]>8253260Alturki, S.M., W.F. Shehata and M.I. Aldaej,2013Phoenix dactylifera L.) cultivars in vitro.]]>12119127Al-Whaibi, M.H.,1990Zyziphus spina-christi L. fruits.]]>295100Al-Whaibi, M.H.,1997919Al-Whaibi, M.H.,200916314Al-Whaibi, M.H. and M.O. Basalah,1986172735Asemota, O., C.R. Eke and J.O. Odewale,2007Phoenix dactylifera L.) in vitro morphogenesis in response to growth regulators, sucrose and nitrogen.]]>623532357Bekheet, S.A. and M.M. Saker,1998Phoenix dactylifera L., cv. Zaghloul.]]>1998pp: 150157Belal, A.H., M.D. El-Deeb and W.F. Shehata,2008in vitro.]]>2008Bhargava, S.C., S.N. Saxena and R. Sharma,2003In vitro multiplication of Phoenix dactylifera (L).]]>124347Cai, Y., Q. Luo, M. Sun and H. Corke,20047421572184Drira, N. and A. Al-Sha'ary,19931993pp: 161170Eriksson, T.,1965Haplopappus gracilis.]]>18976993Eshraghi, P., R. Zarghami and M. Mirabdulbaghi,2005413091312Gamborg, O.L., R.A. Miller and K. Ojima,196850151158Gorinstein, S., Z. Zachwieja, M. Folta, H. Barton, J. Piotrowicz and M. Zemser,200149952957Henriquez, C., S. Almonacid, I. Chiffelle, T. Valenzuela and M. Araya et al.,201070523536Ibrahim, I.A., A.A. Abo-El-Soaud, N.R. El-Sherbeny and E.I. Baker,1999in vitro and ex vitro.]]>1999pp: 209217James, L.F., W.J. Hartley and K.R. Van Kampen,1981178146150Jones, R., T. Mann and R. Sherins,197931531537Kim, D.J. and H.N. Chang,1990Lithospermum erythrorhizon by in situ extraction and calcium alginate immobilization.]]>36460466Lee, Y., D.E. Lee, H.S. Lee, S.K. Kim, W.S. Lee, S.H. Kim and M.W. Kim,2011Morus alba L.).]]>105919Letouze, R., F. Daguin, L. Hamama, K. Paquier, F. Marionnet and M. Javouhey,2000Phoenix dactylifera L.) through somatic embryogenesis: Histological study of embryo formation and cultivar identification by markers.]]>2000pp: 5564Lunde, P.,197829176179Mohamed, M.S.,19961996Rao, S.R. and G.A. Ravishankar,200220101153Sae-Lee, N., O. Kerdchoechuen and N.I. Laohakunjit,201142249252Sarin, R.,200547993SAS 2005Schenk, R.U. and A.C. Hildebrandt,197250199204Sharma, D.R., S. Dawrara and J.B. Chowdury,1984Phoenix dactylifera) cv. Khadrawy through tissue culture.]]>22596598Mohamed, S.M., S.F. El-Sharabasy, H.A. Bosila, I.A. Ibrahim and K.A. Refay,2001cvs of date palm (Phoenix dactylifera L.), 1-Callus initiation and formation.]]>2001pp: 491499Mok, M.C., D.W.S, Mok and D.J. Armstrong,1978Phaseolus.]]>617275Morre, D.J. and J.H. Cherry,19771977pp: 35-43pp: 35-43Murashige, T. and F. Skoog,196215473497Murray, M.G. and J.L. Key,197861190198Nadeem, M., Salim-ur-Rehman, F.M. Anjum and I.A. Bhatti,201149525539Shui, G. and L.P. Leong,200697277284Singleton, V.L. and J.A. Rossi,196516144158Tawaha, K., F.Q. Alali, M. Gharaibeh, M. Mohammad and T. El-Elimat,200710413721378Tisserat, B.,1979Phoenix dactylifera L.) in vitro.]]>3012751283Wajih, S.,19861986Wangensteen, H., A.B. Samuelsen and K.E. Malterud,200488293297Zaid, A.,1984In vitro browning of tissues and media with special emphasis to date palm cultures.]]>3269275Zaid, A.,1989Phoenix dactylifera L.) tissue culture.]]>1989pp: 6775Zaid, A. and E.J. Arias-Jimenez,19991999Zaid, M.,1989Phoenix dactylifera L.)[Research on somatic embryogenesis for date palm (Phoenix dactylifera L.)].]]>1989Zenk, M.H., H. El-Shagi, H. Arens, J. Stockigt, E.W. Weiler and B. Deus,1977Catharanthus roseus.]]>1977pp: 2743Zheng, W. and S.Y. Wang,20014951655170Zhong, J.J.,200172126Shehata, W.F., S.M. Alturki, M.I. Aldaj and H.S. Ghazzawy,2014Phoenix dactylifera L.) in vitro.]]>2014Raghavan, G.S.V., E. McKyes, B. Beaulieu, F. Mfirineau and I. Amir,19761976