Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2014.1170.1178Ginkgo biloba
Leaves]]>SukitoAgus TachibanaSanro 1120141711In vitro α-glucosidase inhibitory activity of
Ginkgo biloba leaves was investigated. The inhibitory activity of methanol
extracts from yellow and green leaves was 13.8 and 40.1 μg mL-1,
respectively. Each methanol extract was separated into its respective fraction
by solvent-solvent extraction with n-hexane, chloroform, ethyl acetate
and n-butanol. The n-hexane fractions (in both methanol extracts
from green and yellow leaves) exhibited high α-glucosidase inhibitory activity
with IC50 values of 13.6 and 13.4 μg mL-1, respectively.
Further fractionation of the n-hexane fractions by silica gel column
chromatography gave the most active fraction which was identified as ginkgolic
acid (C13:0) and a mixture (C13:0, C15:0, C15:1, C17:1 and C17:2). Ginkgolic
acid (C13:0) exhibited the highest α-glucosidase inhibitory activity. This
is the first study to successfully isolate ginkgolic acids as α-glucosidase
inhibitors.]]>Bairy, K.L. and C.M. Rao,2001Ginkgo biloba.]]>12527Baron-Ruppert, G. and N.P. Luepke,2001Ginkgo biloba in the Hen's Egg Test (HET).]]>8133138Bridi, R., F.P. Crossetti, V.M. Steffen and A.T. Henriques,200115449451Cartayrade, A., G. Bourgeois, J.P. Balz and J.P. Carde,1990Ginkgo biloba: Structure, differentiation and analysis of the secretory product.]]>4171178Cheng, D., B. Liang and Y. Li,2013Ginkgo biloba extract in streptozotocin-induced diabetes in rats.]]>2013Christudas, S., L. Gopalakrishnan, P. Mohanraj, K. Kaliyamoorthy and P. Agastian,2009Pisonia alba Span. leaves.]]>64145Doly, M., M.T. Droy-Lefaix, B. Bonhomme and P. Braquet,1986Ginkgo biloba extract on the electrophysiology of the isolated retina from a diabetic rat].]]>1514801483, (In French)EMA,2014Ginkgo biloba L., folium.]]>2014Fuzzati, N., R. Pace and F. Villa,2003Ginkgo biloba extracts.]]>74247256Gholamhoseinian, A., H. Fallah, F. Sharifi-Far and M. Mirtajaddini,20081119Hausen, B.M.,19989146148Hobbs, C.,1991Irie, J., M. Murata and S. Homma,1996Ginkgo biloba.]]>60240243Kennedy, D.O., A.B. Scholey and K.A. Wesnes,2000Ginkgo biloba to healthy young volunteers.]]>151416423Koch, E., H. Jaggy and S.S. Chatterjee,2000Ginkgo biloba L. leaf extracts using the popliteal lymph node assay in the mouse.]]>22229236Kramer, K.U., P.S. Green and E. Gotz,1990pp: 284-289pp: 284-289Kuang, H.X., H.W. Li, Q.H. Wang, B.Y. Yang, Z.B. Wang and Y.G. Xia,2011Sanguisorba tenuifolia var. alba.]]>1646424651Lepoittevin, J.P., C. Benezra and Y. Asakawa,1989Ginkgo biloba L.: Relationship with urushiol.]]>281227230Liu, Z.H. and S. Zeng,2009187131136Lopez-Candales, A.,200132283300Louajri, A., S. Harraga, V. Godot, G. Toubin, J.P. Kantelip and P. Magnin,2001Ginkgo biloba extract on free radical production in hypoxic rats.]]>24710712Mahmoud, F., H. Abul, B. Onadeko, M. Khadadah, D. Haines and G. Morgan,2000In vitro effects of Ginkgolide B on lymphocyte activation in atopic asthma: Comparison with cyclosporin A.]]>83241245Porter, J.R. and T.G. Barrett,200542893902Shankar, P.K., V. Kumar and N. Rao,2005Ginkgo biloba in streptozotocin induced diabetic rats.]]>41619Shim, Y.J., H.K. Doo, S.Y. Ahn, Y.S. Kim, J.K. Seong, I.S. Park and B.H. Min,200385283287Silverstein, R.M., F.X. Webster and D.J. Kiemle,20057th Edn.,Pages: 502Pages: 502Singh, B., P. Kaur, R.D. Singh and P.S. Ahuja,2008Ginkgo biloba.]]>79401418Uddin, G., A. Rauf, A.M. Al-Othman, S. Collina, M. Arfan, G. Ali and I. Khan,2012Pistacia integerrima.]]>8316481652Van Beek, T.A. and M.S. Wintermans,2001Ginkgo biloba.]]>930109117Zhang, H., H. Matsuda, C. Yamashita, S. Nakamura and M. Yoshikawa,2009Hydrangea macrophylla var. thunbergii as a new type of anti-diabetic compound.]]>606255261