Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2013.86.91DeoreG.B. LimayeA.S. DushingY.A. DhobaleS.B. KaleS. LawareS.L. 22013162The problem of metalnutrient deficiency is becoming more serious with the introduction of modern agricultural practices. As a result, metalnutrient deficiency is recognized as one of the critical yield limiting factors. Metalnutrients are generally offered in their sulphate or oxide forms. However, it is reported that organically bound minerals generally have a higher bioavailability than inorganic minerals. Chelation makes otherwise unavailable metalnutrients plant available. Amino acids are well known among various chelating agents. In present investigation the fungus Paecilomyces variotii PR-4 was isolated from soil and was used for production of protease and determination of its activity. Proteins from germinating seeds of chick pea, mung bean, soybean and cowpea were hydrolyzed for the production of amino acids. Amino acids were recovered, estimated and utilized for chelation of metalnutrients viz., Zn, Cu, Fe, Mn, Mg, B and Mo. The resultant chelates were employed to detect with Fourier Transform Infra-Red Spectrophotometer (FTIR) analysis. The peaks of most intensive bands in the IR spectra of ligands recorded were present in the intervals of the wave numbers 3500-3300 and 1720-1700 cm-1. Chelation of metalnutrients led to the broadening of peak and changes of the peak position of hydroxyl groups, which indicated the binding of the carboxylic groups and primary amine groups of amino acids to the metalnutrients. The resultant amino acids-metalnutrient chelates can be utilized as organic fertilizer.]]>AAPFCO,19981998Sumantha, A., P. Deepa, C. Sandhya, G. Szakacs, C.R. Soccol and A. Pandey,200649843851Amersham,19981998Aoki, K., T. Tanaka, R. Shinke and H. Nishira,1979170446448Araki, K. and T. Ozeki,19911991Ashmead, H.D.,19861986pp: 1-9pp: 1-9Ayet, G., C. Burbano, C. Cuadrado, M.M. Pedrosa and L.M. Robredo et al.,1997Lens culinaris).]]>74273279Baker, D.H. and C.B. Ammerman,19951995pp: 367-392pp: 367-392Benepal, P.S.,196744363369Bose, U.S. and S.K. Tripathi,1996126164Ellaiah, P., K. Adinarayana, Y. Bhavani, P. Padmaja and B. Srinivasulu,2002Aspergillus species.]]>38615620Fageria, N.K., V.C. Baligar and R.B. Clark,200277185268Foster, A.S.,193499192Ghanem, K.Z. and L. Hussein,199950351356Hajji, M., A. Rebai, N. Gharsallah and M. Nasri,2008Aspergillus clavatus ES1 in Mirabilis jalapa tuber powder using statistical experimental design.]]>79915923Jansman, A.J.M.,199611429432Jeppsen, R.B.,19911991Joo, H.S., G.C. Park, K.M. Kim, S.R. Paik and C.S. Chang,2001Periserrula leucophryna: Purification and characterization.]]>36893900Kalisz, H.M.,19881988pp: 3-61pp: 3-61Kataria, A., B.M. Chauhan and D. Punia,1989in vitro) of mungbean as affected by domestic processing and cooking.]]>32917Kemp, W.,1998Madan, M., S. Dhillon and R. Singh,2002Bacillus polymyxa.]]>42155159Manachini, P.L., M.G. Fortina and C. Parini,1988Bacillus thermorubber: A new species of Bacillus.]]>28409413Meister, R.T.,1999Miller, G.W.,19981998Morgan, G.T. and H.D.K. Drew,192011714561464Naidu, K.S.B. and K.L. Devi,20054724726Nakamoto, K.,19861st Edn.,Norton, G., F.A. Bliss and R. Bressani,19851985pp: 73-114pp: 73-114Osawa, R. and T.P. Walsh,19935912511252Paranthaman, R., K. Alagusundaram and J. Indhumathi,2009Aspergillus niger in solid state fermentation.]]>5308312Patil, B.C., R.M. Hosamani, P.S. Ajjappalavara, B.H. Naik, R.P. Smitha and K.C. Ukkund,2008Lycopersicon Esculentum Mill.).]]>21428430Preet, K. and D. Punia,2000in vitro) of soaked, dehulled and germinated cowpea.]]>14109117Sadasivam, S. and A. Manickam,20033rd Edn.,Sandhya, C., A. Sumantha, G. Szakacs and A. Pandey,2005Aspergillus oryzae in submerged and solid-state fermentation.]]>4026892694Sattar, A., S.K. Durrani, F. Mahmood, A. Ahmad and I. Khan,198934111120Srinubabu, G., N. Lokeswari and K. Jayaraju,2007Aspergillus oryzae.]]>4208215Vanlada, S., N. Pracha, P. Siangjeaw and M. Leka,2006Trichoderma viride and Azomonas agilis from vetiver rhizosphere.]]>2006Vanderstoep, J.,1981388385