Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2013.720.725SahaTapabrata GuhaChanchal ChakrabortyDhruba PalBiplab BiswasUjjwal ChatterjeeAmaresh KoenigPatricia BeerMartin 1220131615Infectious Bovine Rhinotracheitis (BoHV-1) is the most important emerging disease of cattle in India. With an aim to reactivate BoHV-1 from latently infected sero-positive cattle for molecular characteristics of the isolates prevalent in tropical and sub-tropical countries like India and further epidemiological investigations on IBR infections this study had been conducted. Artificial stress with dexamethasone at the dose rate of 0.1 mg kg-1 body weight for 5 consecutive days was induced in BoHV-1 sero-positive cows. Then isolation from nasal swabs was attempted in Madin Darby Bovine Kidney (MDBK) cell line to find out the prevalent strain in India. The virus was isolated from all the three cows. All the three isolates were typed as BoHV-1.2 (Strain India 4, India 5 and India 6). The reactivation obtained in this study with dexamethasone suggests the usefulness of BoHV-1 cow latency model for epidemiological investigations on BoHV-1 infections in tropical and sub-tropical countries like India, Pakistan etc.]]>Ackermann, M., E. Peterhans and R. Wyler,1982433640Ackermann, M. and M. Engels,2006113293302ADMAS,20032003RDDL,20042004RDDL,20052005RDDL,20062006Chinchkar, S.R., V.V. Deshmukh, Abdulaziz and M.B. Gujar,2002797879Davies, D.H. and I.E. Carmichael,19738510518Dhand, N.K., G. Singh, D.R. Sharma and K.S. Sandhu,200272850852De Carlo, E., G.N. Re, R. Letteriello, V. Del Vecchio and M.P. Giordanelli et al.,2004Babalusbubalis) after pharmacological reactivation.]]>154171174Gibbs, E.P.J. and M.M. Rweyemamu,197747317343Gill, B.S., D.R. Sharma, M.S. Kwatra, A. Kumar and J.S. Gill,198724317318Hage, J.J., R.D. Glas, H.H. Westra, M.A. Maris-Veldhuis, J.T. Van Oirschot and F.A.M. Rijsewijk,1998608798Kumar, M., M. Rajasekhar and G. Krisnappa,199471109112Kilari, S., S.N. Singh, A.V. Buche, S. Sawarkar and S. Ghosh,200077185188Mehrotra, M.L., B.S. Rajya and S. Kumar,197617073Mehrotra, M.L. and B.S. Rajya,198151561562Mohan, M., B.K. Singh and R. Manickam,198966914916OIE,2009pp: 752-767pp: 752-767Pastoret, P.P., L.A. Babiuk, V. Misra and P. Griebel,198029483488Pastoret, P.P., E. Thiry and R. Thomas,198667885897Pritchard, G., N. Cook and M. Banks,1997140587587Pistl, J., M. Bobakova, P. Mudron and E. Pilipcinec,2003282428Rock, D., J. Lokensgard, T. Lewis and G. Kutish,19926624842490Radostits, O.M., C.C. Gay, D.C. Blood and K.W. Hinchcliff,20009th Edn.,pp: 1173-1184pp: 1173-1184Rajesh, J.B., P.V. Tresamol and M.R. Saseendranath,200380393396Rola, J., M. Larska and M.P. Polak,200549267271Saha, T., C. Guha, D. Chakraborty, B. Pal and U. Biswas et al.,2010218SuriBabu, T., B.B. Mallick and S.K. Das,198461195200Satyanarayana, K. and T. SuriBabu,198757499502Straub, O.C. and R.J. Lorenz,199146344354Suresh, K.B., K.J. Sudharshana and M. Rajasekhar,19997659 Six, A., M. Banks, M. Engels, C. RosBascunana and M. Ackermann,200114613251335Vaid, J., L. Krishna and R.S. Kaushal,199168705707