Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2013.396.400Vatica diospyroides Symington: An Endangered Medicinal Dipterocarp of Peninsular Thailand by Cultures of Embryonic Axes and Leaf-derived Calli]]>SrisawatTheera JongkraijakNathinan 82013168Embryonic axes and young leaves of Vatica diospyroides Symington were excised sterilely and then cultured on MS medium supplemented with 0-20 mg L-1 of a growth regulator and 0-0.3% Activated Charcoal (AC). The growth regulators tested were 2, 4-Dichlorophenoxyacetic acid (2,4-D), α-Naphthaleneacetic acid (NAA) and 3, 6-Dichloro-2-methoxybensoic acid (Dicamba). Vigorous shoot development was observed with 0-20 mg L-1 of each plant growth regulator and 0.1-0.15% AC. Shoot-derived calli were obtained 6 months after culturing embryonic axes on MS medium, with 15 mg L-1 dicamba and 0.1% AC. Interestingly, after culturing young leaves for 2 months, the highest weight of compact calli (0.40 g) was achieved with a medium containing 20 mg L-1 dicamba and 0.1% AC, in darkness. These were successfully multiplied by renewing and culturing in the same medium and transfer to shooting induction in MS medium supplemented with 0-20 mg L-1 6-benzyladenine (BA). Unfortunately, shoot induction from calli was unsuccessful and despite initiated roots being induced. A successful in vitro propagation protocol of V. diospyroides should be thus investigated more extensively.]]>Abdulhadi, R., K. Kartawinata and S. Sukardjo,198144407418Aguado-Santacruz, G.A., A. Velazquez-Ordinola, B. Moreno-Gomez, L.M. Gomez-Torres, L.F. Diaz-Espino and F.P.G. Vazquez,2011in vitro plant regeneration systems for elite malting barley varieties: Optimizing media formulation and explant selection.]]>101952219533Ashton, P.,19981998Atun, S., N. Aznam, R. Arianingrum, Y. Takaya and N. Masatake,2008Hopea and their biological activity test.]]>19721Bahieldin, A., W.E. Dyer and R. Qu,2000119437439Brisibe, E.A., H. Miyake, T. Taniguchi and E. Maeda,1994Saccharum officinarum L.).]]>126301307Gorji, A.H., M. Zolnoori, A. Jamasbi and Z. Zolnoori,2011In vitro plant generation of tropical maize genotypes.]]>2011pp: 5259Ito, T., Y. Akao, H. Yi, K. Ohguchi and K. Matsumoto et al.,20032414891497Kackar, A., S.R. Bhat, K.P.S. Chandel and S.K. Malik,199332289292Kinghorn, A.D.,20005285288Linington, I.M.,1991In vitro propagation of Dipterocarpus alatus and Dipterocarpus intricatus.]]>278188Lloyd, G. and B. McCown,1980Kalmia latifolia, by use of shoot-tip culture.]]>30421426Moura-Costa, P. and L. Lundoh,1994Dryobalanops lanceolata (Dipterocarpaceae) by cuttings.]]>6533541Murashige, T. and F. Skoog,196215473497Pooma, R.,200230727Satyavathi, V.V., P.P. Jauhar, E.M. Elias and M.B. Rao, 2004in vitro plant regeneration in durum wheat. ]]>4418391846Seo, E.K., H. Chai, H.L. Constant, T. Santisuk and V. Reutrakul et al.,1999Vatica diospyroides.]]>6469766983Scott, E.S., A.N. Rao and C.S. Loh,1988Shorea roxburghii G. Don. from embryonic axes cultured in vitro.]]>61233236Scott, E.S., A.N. Rao and C.S. Loh,1995Hopea odorata, a dipterocarp tree.]]>41193196Smith, M.T., B.S.P. Wang and H.P. Msanga,20022002pp: 149 -176pp: 149 -176Smits, W.T.M. and B. Struycken,1983in vitro culture of dipterocarps.]]>31233238Smitinand, T.,19661966pp: 6776Srisawat, T., S. Thipnetr and C. Maknoi,2012Vatica diospyroides Symington, endangered medicinal plant of Peninsular Thailand.]]>636813688Srisawat, T., P. Chumkaew, W. Heed-Chim, Y. Sukpondma and K. Kanokwiroon2013Vatica diospyroides Symington type LS.]]>127176Thomas, T.D.,200826618631Vaario, L.M.,1996Betula platyphylla var. japonica and in Dipterocarpaceae Species.]]>9651118Zain, W.Z.W.M., N. Ahmat, N.H. Norizan and N.A.A.M. Nazri,20115926929Wu, J.M., T.C. Hsieh and Z. Wang,201113847