Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2013.281.286GolalipourM.J. GhafariS. KafshgiriS. Kaboli MoghadamM.H. Latifi MoharriA.R. 62013166This study carried out to evaluate the effects of maternal morphine exposure during gestational and lactation period on the neuronal cells of dentate gyrus in 18 and 32 days Balb/c mice offspring. In this experimental study 10 female mice were randomly allocated into cases and controls. In experimental group, animals were received morphine sulfate 10 mg/kg/body weight intraperitoneally during 7 days before mating, gestational period (GD0-21), 18 and 32 days after delivery. The control animals were received an equivalent volume normal saline. Cerebrum of six infant for each group were removed and stained with cresyl violet and monoclonal anti-neuronal nuclei (NeuN) antibody. Quantitative computer-assisted morphometric study was done on dentate gyrus of hippocampus. In the P18 mice , the numbers of granular cells in dentate gyrus medial blade and dentate gyrus lateral blade significantly reduced from 171.45±4.2 and 174.51±3.1 cells in control group to 153.32±2.8 and 151.23±3.2 cells in 10000 μm2 area of granular layer in treated group (p<0.001). In P32 mice the numbers of granular cells in mb and lb of dentate gyrus significantly decreased from 155.31±4.1 and 153.77±3.4 in control group to 138.33±4.5 and 135.13±4.3 in treated group, respectively (p<0.001). The granular layer thickness in mb and lb area of dentate gyrus significantly reduced in treated mice in compared to controls in P18 and P32 mice (p<0.05). This study revealed that morphine administration before, during pregnancy and lactation period causes neuronal cells loss of dentate gyrus in 18 and 32 days old infant mice.]]>Atici, S., L. Cinel, I. Cinel, N. Doruk, M. Aktekin, A. Akca, H. Camdeviren and U. Oral,200411410011011Bekheet, S.H., S.A. Saker, A.M. Abdel-Kader and A.E. Younis,201042165175Cameron, H.A. and E. Gould,199461203209Cheng, W.H., F.W. Quimby and X.G. Lei,200334918927Eisch, A.J., M. Barrot, C.A. Schad, D.W. Self and E.J. Nestler,20009775797584Farber, N.B. and J.W. Onley,20031473745Garcia, M.M., J. Gilster and R.E. Harlan,199628k immunoreactivity in a subset of cerebellar Purkinje neurons of rat brain.]]>734123134Ghafari, S., D. Roshandel and M.J. Golalipour,201149328334Gould, E. and P. Tanapat,199780427436Gould, E., P. Tanapat, T. Rydel and N. Hastings,200048715720Gould, E. and C.G. Gross,200222619623Hammer, R.P.J., A.A. Ricalde and J.V. Seatriz,198910475483Hauser, K.F., J.A. Gurwell and C.S. Turbek,199413095105Hauser, K.F., P.J. McLaughlin and I.S. Zagon,1987416157161Hauser, K.F., M.E. Harris-white, J.A. Jackson, L.A.Opanashuk and J.M.Carney,19982+ homeostasis and induce carbonyl oxyradical production in mouse astrocytes In vitro: Transient increases and adaptation to sustained exposure.]]>1517076Hauser, K.F., A.A. Houdi, C.S. Turbeck, R.P. Elde and W. Maxson,2000In vitro: Differential impact of μ and δ receptor activation on proliferation and neurite elongation.]]>1212811293Jackson-Guilford, J., J.D. Leander and L.K. Nisenbaum,20002939194Jacob, J.T.,20072007Lorber, B.A., S.K. Freitag and J.V. Bartolome,1990531329332Mao, J., B. Sung, RR. Ji and G. Lim,20022276507661Mei, B., L. Niu, B. Cao, D. Huang and Y. Zhou,20096311541161Morris, R.G.M., E. Anderson, G.S. Lynch and M. Baudry,1986319774776Morris, R.G.,1989in vivo by the N-methyl-D-aspartate receptor antagonist AP5.]]>930403057NIDA,19961996Nestler, E.J.,200425210218Niu, L., B. Cao, H. Zhu, B. Mei, M. Wang, Y. Yang and Y. Zhou,2009in vivo synaptic plasticity in dentate gyrus and spatial memory in juvenile rats induced by prenatal morphine exposure.]]>19649657Ornoy, A., V. Michailevskaya, I. Lukashov, R. Bar-Hamburger and S. Harel,199620385396Emeterio, E.P.S., M. Tramullas and M.A. Hurle,20068313521361Pretorius, E. and M.S. Bornman,200565893904Seatriz, J.V. and R.P. Hammer,199330523527Schmahl, W., R. Funk, U. Miaskowski and J. Plendl,1989486297300Silva, A.J., R. Paylor, J.M. Wehner and S. Tonegawa,1992257206211Svensson, A.L., N. Bucht, M. Hallberg and F. Nyberg,200810573047308Turchan-Cholewo, J., Y. Liu, S. Gartner, R. Reid and C. Jie et al.,200623109119United Nations Office on Drugs and Crime,20062006Wilson, G.S., R. McCreay, J. Kean and J.C. Baxter,197963135141Yang, S.N., L.T. Huang, C.L. Wang, W.F. Chen and C.H. Yang et al.,200313915921Yang, S.N., C.A. Liu, M.Y. Chung, H.C. Huang and G.C. Yeh et al.,200616521530Zagon, I.S. and P.J. MacLaughlin,19874126872Zagon, I.S. and P.J. MacLaughlin,19921992pp: 241-282pp: 241-282Zhang, Y., Q. Chen and L.C. Yu,200814561570