Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2013.1971.1977In-vitro Antioxidant Activity Isolated Bioactive Compounds from Tridax procumbens Linn]]>SaxenaManjusha MirAbrar Hussain SharmaManik Yousuf MallaMohd QureshiSumeerah MirMohd Iqbal ChaturvediYogesh 1220131624Tridax procumbens L., Asteraceae, has been extensively used for various ailments in the Ayurvedic system of medicine. Previous studies have revealed remarkable phytoconstituents from Tridax procumbens L. with significant antioxidant activity. The aim of the present study is to measure the anti-DPPH activity of the purified isolated compounds from n butanol soluble part and ethyl acetate soluble part of successive methanolic extract of Tridax procumbens L. We thus quantified the total phenolic and total flavonoids in different purified isolated compounds, the whole of the tests were evaluated with a sample conc. of 100 μg mL-1 and were determined spectrophotometrically using Folin-ciocaltue and AlCl3 reagents, respectively. DPPH (1, 1-diphenyl, 2-picryl hydrazyl) assay was used to determine the in vitro antioxidant activity of different isolated compounds. Isolated compounds, one from ethyl acetate soluble part (EF-I) and one from n butanol soluble part (BF-II) were reported to possess a significant anti DPPH activity with lowest IC50 values 67.26 and 80.90 μg mL-1, respectively while comparable to standard ascorbic acid with IC50 value of 59.62 μg mL-1, due to the high concentration of phenols 146.4 μg mL-1 from EF-I and 142.2 μg mL-1 from BF-II and flavonoids 48 and 42.5 μg mL-1 found in EF-I and BF-II isolated compounds, respectively.]]>Imam, S., I. Azhar, M.M. Hasan, M.S. Ali and S.W. Ahmed,2007Tamarindus indica linn.]]>20125127Yildirim, A., M. Oktay and V. Bilaloglu,2001Cydonia vulganis.]]>312327Alshawsh, M.A., M.A. Abdulla, S. Ismail, Z.A. Amin, S.W. Qader, H.A. Hadi and N.S. Harmal,2012Orthosiphon stamineus.]]>1753855395AYUSH,20122012Bhagwat, D.A., S.G. Killedar and R.S. Adnaik,2008Tridax procumbens.]]>2126128Bhat, R.S., J. Shankrappa and H.G. Shivakumar,200721117Blois, M.S.,2001in vitro.]]>5510181018Evans, W.C. and G.E. Trease,198916th Edn.,GOI,20012001Harborne, J.B.,19842nd Edn.,Jude, C.I., C.I. Catherine and M.I. Ngozi,2009Tridax procumbens Linn.]]>8548550Kunle, O.F., H.O. Egharevba and P.O. Ahmadu,20124101112Nia, R., D.H. Paper, E.E. Essien, O.H. Oladimeji, K.C. Iyadi and G. Franz,2003in-vitro radical scavenging and in-vivo anti-inflammatory potential of Tridax Procumbens.]]>183943Oladunmoye, M.K.,2006Tridax procumbens on swiss alblno rats orogastrically dosed with Pseudomonas aeruginosa (NCIB 950).]]>1152155Prabhu, V.V., G. Nalini, N. Chidambaranathan and S.S. Kisan,2011tridax procumbens Linn against formalin, acetic acid and cfa induced pain models.]]>3126130Quettier-Deleu, C., B. Gressier, J. Vasseur, T. Dine and C. Brunet et al.,2000Fagopyrum esculentum Moench) hulls and flour.]]>723542Racek, J., H. Rusnakova, L. Tretil and K.K. Siala,2005548795Chander, R., A.K. Khanna, K. Raj and A.K. Rastogi,200520153159Ravikumar, V., K.S. Shivashangari and T. Devaki,2005Tridax procumbens against D-galactosamine/lipopolysaccharide-induced hepatitis in rats.]]>1015560Ravishankara, M.N., N. Shrivastava, H. Padh and M. Rajani,2002Hemidesmus indicus R. Br. (Anantmul).]]>9153160Rice-Evans, C.A., N.J. Miller, P.G. Bolwell, P.M. Bramley and J.B. Pridham,199522375383Sahoo, G. and P.K. Chand,1998In Vitro photifilication of a medicine herbs Tridax procumbens: Influence of explanting season growth regulator synergy culture passage and planting substance.]]>48195205Seebacher, W., N. Simic, R. Weis, R. Saf and O. Kunert,200341636638Singleton, V.L., R. Orthofer and R.M. Lamuela-Raventos,19991999pp: 152-178pp: 152-178Taddei, A. and A.J. Rosas-Romero,2000Tridax procumbens.]]>7235238NISCAIR,20042004Tiwari, U., B. Rastogi, P. Singh, D.K. Saraf and S.P. Vyas,2004Tridax procumbens in experimental animals.]]>92113119Verma, R.K. and M.M. Gupta,1988Tridax procumbens.]]>27459463Vishnu, P.P., K. Radhika, K.R. Siva, S.M. Ramchandra, D.Y. Prameela and R.A. Srinivas,2011Tridax procumbens flower extracts on PC 3 cell lines.]]>22630Wang, H., F. Liu, L. Yang, Y.G. Zu, H. Wang, S.Z. Qu and Y. Zhang,20111289399