Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2013.1751.1757SessouP. FarougouS. AhounouS. HounnankponY. AzokpotaP. YoussaoI. SohounhloueD. 1220131623The study has compared the antifungal efficacy of six essential oils, Cinnamomum zeylanicum, Cymbopogon citratus, Ocimum gratissimum, Pimenta racemosa, Syzygium aromaticum and Zingiber officinale, tested in culture medium and in traditional cheese wagashi system against moulds belonging to Aspergillus, Penicillium, Fusarium and Scopulariopsis genera in perspective to select the most actives as substitutes of chemical preservatives for wagashi preservation. Results obtained from this work indicated that Syzygium aromaticum, Pimenta racemosa, Ocimum gratissimum and Cymbopogon citratus essentials oils were the most actives extracts at in vitro assay in decreasing order with strong fungistatic activity against the isolates tested; the pronounced activity was provided by S. aromaticum essential oil. The effectiveness of these actives oils on the less sensitive moulds common to these oils showed that, among these extracts that of Syzygium aromaticum in particular exerted high sporale reduction against all the strains tested. In sum, Syzygium aromaticum essential oil possessed the highest antifungal activity both in culture medium and in wagashi system. Essential oils of C. citratus, O. gratissimum, P. racemosa and above all that of S. aromaticum, among the six extracts investigated, were the most promising oils as wagashi additives in substitution of synthetic chemicals ones to extend shelf life time of this by-product of milk for its valorization. Further studies are needed to be performed on the safety of oils for human, the shelf life time of this cheese and its acceptability when treated with essential oils to reduce and control pathogen contamination or native microflora.]]>Aissi, V.M., M.M. Soumanou, H. Bankole, F. Toukourou and C.A. de Souza,2009323972404Alzoreky, N.S. and K. Nakahara,200380223230Angelini, P., R. Pagiotti, A. Menghini and B. Vianello,2006566569Bakkali, F., S. Averbeck, D. Averbeck and M. Idaomar,200846446475Barkat, M. and A. Bouguerra,2012Foeniculum vulgare Mill. for its use as food conservative.]]>6239244Burt, S.,200494223253Food and Drug Administration,20052005Gandomi, H., A. Misaghi, A.A. Basti, S. Bokaei, A. Khosravi, A. Abbasifar and A.J. Javan,2009Zataria multiflora Boiss. essential oil on growth and aflatoxin formation by Aspergillus flavus in culture media and cheese.]]>4723972400Goni, P., P. Lopez, C. Sanchez, R. Gomez-Lus, R. Becerril and C. Nerin,2009116982989Hsouna, A.B., Trigui, R.B. Mansour, R.M. Jarraya, M. Damak and S. Jaoua,2011Ceratonia siliqua essential oil with preservative effects against Listeria inoculated in minced beef meat.]]>1486672ICMSF,20052nd Edn.,Pages: 777Pages: 777Keke, M., B. Yehouenou, E. Dahouenon, J. Dossou and D.C.K. Sohounhloue,2008Lactobacillus plantarum.]]>107386Lopez-Reyes, J.G., D. Spadaro, M.L. Gullino and A. Garibaldi,2010in vivo.]]>25171177Lv, F., H. Liang, Q. Yuan and C. Li,2011In vitro antimicrobial effects and mechanism of action of selected plant essential oil combinations against four food-related microorganisms.]]>4430573064Nguefack, J., S.K. Nguikwie, D. Fotio, B. Dongmo and P.H.A. Zollo et al.,2007Cymbopogon citratus, Ocimum gratissimum and Thymus vulgaris to control Alternaria padwickii and Bipolaris oryzae, two seed-borne fungi of rice (Oryza sativa L.).]]>19581587Pinto, E., L. Vale-Silva, C. Cavaleiro and L. Salgueiro,2009Syzygium aromaticum on Candida, Aspergillus and dermatophyte species.]]>5814541462Philippe, S., F. Souaibou, A. Guy, D.T. Sebastien and Y. Boniface et al.,2012Ocimum gratissimum from Benin against six mycotoxigenic fungi isolated from traditional cheese wagashi.]]>12227Sessou, P., S. Farougou, P. Azokpota, I. Youssao and B. Fanou et al.,2012In vitro antifungal activity of essential oil of Pimenta racemosa against fungal isolates from wagashi, a traditional cheese produced in Benin.]]>82534.Sessou, P., S. Farougou, P. Azokpota, I. Youssao and D. Sohounhloue,2012In vitro antifungal activities of essential oils extracted from fresh leaves of Cinnamomum zeylanicum and Ocimum gratissimum against foodborne pathogens for their use as traditional cheese wagashi conservatives.]]>16773Sessou, P., S. Farougou, J.P. Noudogbessi, B. Fanou, P. Azokpota, I. Youssao and D. Sohounhloue,2012in vitro antifungal activity of Zingiber officinale essential oil against foodborne pathogens isolated from a traditional cheese wagashi produced in Benin.]]>22028Sessou, P., S. Farougou and D. Sohounhloue,201224557Sessou, P., S. Farougou, S. Kaneho, S. Djenontin and G.A. Alitonou et al.,2012Cymbopogon citratus essential oil against foodborne pathogens in culture medium and in traditional cheese wagashi produced in Benin.]]>3406415Sessou, P., S. Farougou, B. Yehouenou, B. Agniwo and G.A. Alitonou et al.,2013Syzygium aromaticum essential oil.]]>2013Singh, P., R. Shukla, B. Prakash, A. Kumar, S. Singh, P.K. Mishra and N.K. Dubey,2010Citrus maxima Burm. and Citrus sinensis (L.) Osbeck essential oils and their cyclic monoterpene, DL-limonene.]]>4817341740SAS,19894th Edn.,StatSoft,20102010Tatsadjieu, N.L., J.P.M. Dingamo, M.B. Ngassoum, F.X. Etoa and C.M.F. Mbofung,2009Lippia rugosa from Cameroon for its potential use as antifungal agent against Aspergillus flavus Link ex. Fries.]]>20161166Varona, S., R.S. Rojo, A. Martin, J.M. Cocero and T.A. Serra et al.,201342243250Velazquez-Nunez, M.J., R. Avila-Sosa, E. Palou and A. Lopez-Malo,2013Citrus sinensis var. Valencia) peel essential oil applied by direct addition or vapor contact.]]>3114WHO,2002Yehouenou, B., E. Ahoussi, P. Sessou, F. Toukourou and D. Sohounhloue,2012654965505Boniface, Y., S. Philippe, H.R. de Lima, N.J. Pierre, A.G. Alain, T. Fatiou and S. Dominique,2012Cinnamomum zeylanicum Blume dry leaves essential oil against food-borne pathogens and adulterated microorganisms.]]>11825