Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2013.1303.1310Aeromonas hydrophila
Strains and the Varied Responses of the Strains Towards Phyto-extracts]]>BalasundaramA. Rathna KumariP. KolanchinathanP. MasilamaniV. JohnGeorge 1220131621The present study evaluated genetic variation in Aeromonas
hydrophila strains using PCR-RAPD and their varied susceptibility to phyto-extract.
Four strains of Aeromonas hydrophila isolated from skin infections of
common freshwater fish, Cyprinus carpio were characterized by various
biochemical methods, physiological tests and PCR- RAPD. Antimicrobial activity
of the leaf extracts of three medicinal plants, Ocimum sanctum, Adathoda
vasica and Calendula officinalis were tested against the four strains
of A. hydrophila by disc diffusion (Kirby-Bauer) method. Antagonistic
effects of leaf extracts against A. hydrophila strains were assessed
by co-culture method. RAPD analysis showed that all the microbes isolated from
skin infection belong to the same species but there was no 100% genetic similarity
among them Dendrogram constructed by UPGMA clearly supported the PCR pattern
of genetic variability among the strains. This study revealed that Aeromonas
hydophila exhibits genetic variability and varied susceptibility towards
phyto-extracts. Results indicated that phyto-extracts offers a promising alternative
to the use of antibiotics in controlling Aeromonas hydrophila.]]>Aguilera-Arreola, M.G., C. Hernandez-Rodriguez, G. Zuniga, M.J. Figueras and G. Castro-Escarpulli,2005Aeromonas hydrophila clinical and environmental ecotypes as revealed by genetic diversity and virulence genes.]]>242231240Pereira, R.S., T.C. Sumita, M.R. Furlan, A.O. Jorge and M. Ueno,200438326328Christybapita, D., M. Divyagnaneswari and R.D. Michael,2007Eclipta alba leaf aqueous extract enhances the non specific immune responses and disease resistance of Oreochromis mossambicus.]]>23840852Davis, W.A., J.G. Kane and V.F. Garagusi,1978Aeromonas infections: A review of the literature and a case report of endocarditis.]]>57267277Divyagnaneswari, M., D. Christybapita and R.D. Michael,2007Solanum trilobatum leaf fractions.]]>23249259Fainstein, V., S. Weaver and G.P. Bodey,1982In vitro susceptibility of Aeromonas hydrophila against new antibiotics.]]>22513514Fass, R.J. and J. Barnishan,1981In vitro susceptibility of Aeromonas hydrophila to 32 antimicrobial agents.]]>19357361Fraire, A.E.,1978Aeromonas hydrophila infection.]]>239192192Garcia-Vallve, S., J. Paulu and A. Romeu,19991611251134Garcia, J.A., J.L. Larsen, I. Dalsgaard and K. Pedersen,2000Aeromonas salmonicida ssp. Salmonicida.]]>190163166Gerin, M.,199919993240Hazen, T.C., C.B. Fliermans, R.P. Hirsch and G.W. Esch,1978Aeromonas hydrophila in the United States.]]>36731738Hayashi, F., Y. Araki, K. Harada, M. Inoue and S. Mitshuhashi,19824811211127Holt, J.G., N.R. Kreig, P.H.A. Sneath and J.T. Staley,19941994pp: 255-273pp: 255-273Itami, T., M. Asano, K. Tokushige, K. Kubano and A. Nakawaga et al.,1998Penaeus japonicus, after oral administration of peptidoglycan derived from Bifidobacterium thermophilum.]]>164277288Kaper, J.B., H. Lockman, R.R. Colwell and S.W. Joseph,1980Aeromonas hydrophila: Ecology and toxigenicity of isolates form an estuary.]]>50359377Larsen, J.L. and N.J. Jensen,1977Aeromonas species implicated in ulcer-disease of the cod (Gadus morhua).]]>29199211Lee, S., S. Kim, Y. Oh and Y. Lee,2000Aeromonas hydrophila isolated from rainbow trouts in Korea.]]>3817Logambal, S.M., S. Venkatalakshmi and R.D. Michael,2000Ocimum sanctum Linn. Oreochromis mossambicus (Peters).]]>430113120Mathewson, J.J. and H.L. Dupont,1992Aeromonas Species: Role as Human Pathogens.]]>1992pp: 26-36pp: 26-36Muyzer, G., E.C. de Waal and A.G. Uitterlinden,199359695700McNicol, L.A., K.M. Aziz, I. Huq, J.B. Kaper and H.A. Lockman et al.,1980Aeromonas hydrophila from aquatic environments.]]>17477483Miyata, M., T. Aoki, V. Inglis, T. Yoshida and M. Endo,1995Aeromonas salmonicida and Aeromonas hydrophila.]]>79181185Miyazaki, T. and N. Kaige,1985Crucian carp.]]>21181185CLSI20022002Palumbo, S.A., M.M. Bencivengo, F. Del Corral, A.C. Williams and R.L. Buchanan,1989Aeromonas hydrophila group isolated from retail foods of animal origin.]]>27854859Rahim, Z., S.C. Sanyal, K.M. Aziz, M.I. Huq and A.A. Chowdhury,1984Aeromonas hydrophila strains from infected fish in Bangladesh.]]>48865867Ramesh, N., M.B. Viswanathan, A. Sarasawathy, K. Balakrishna, P. Brindha and P. Lakshmanaperumalsamy,2002Begonia malabarica.]]>79129132Rao, Y.V., B.K. Das, J. Pradhan and R. Chakrabarti,2006Achyranthes aspera on the immunity and survival of Labeo rohita infected with Aeromonas hydrophila.]]>20263273Rogol, M., I. Sechter, L. Grinber and C.B. Gerichter,1979Aeromonas hydrophila.]]>12229231Salyers, A.A. and D.D. Whitt,1994Samy, R.P. and S. Ignacimuthu,2000696371Bonjar, G.H.S. and A.K. Nik,2004Pseudomonas aeruginosa and P. fluorescens.]]>36164Szczuka, E. and A.Kaznowski,2004Aeromonas sp. strains by random amplified polymorphic DNA PCR, repetitive extragenic palindromic PCR and enterobacterial repetitive intergenic consensus sequence PCR.]]>42220228Szczuka, E. and A. Kaznowski,2007Aeromonas caviae and A. veronii by standardized cellular protein electrophoretic patterns.]]>526569Van der Kooij, D.,1988187117Williams, J.G.K., A.R. Kubelik, K.J. Livak, J.A. Rafalski and S.V. Tingey,19901865316535