Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2011.894.899in vitro Amylase Inhibitory Effect of the Leaves of Breynia retusa]]>KripaK.G. SangeethaR. MadhaviP. DeepthiP. 1220111419This study was proposed based on the folklore claim and on the scarcity of scientific evidence from the literature for the medicinal uses of Breynia retusa. The aim of the present study was to analyse the phytochemical constituents of the leaves of B. retusa. The fractions obtained by successive fractionation using solvents of varying polarity were studied for the presence of primary and secondary metabolites and the total phenolic content of the different fractions were determined by HPLC. The results of the study support the traditional acclaim of the therapeutic uses of B. retusa. The potential of B. retusa to inhibit α-amylase, a prime enzyme involved in carbohydrate metabolism was analysed and it was observed that the ethyl acetate and methanolic extract of the leaves of B. retusa possessed in vitro amylase inhibitory activity.]]>Kumar, M.S.B. and N.P. Balakrishnan,1996Euphorbia L. (Euphorbiaceae).]]>124649Franco, F.M. and D. Narasimhan,20098645648Laloo, R.C., L. Kharlukhi, S. Jeeva and B.P. Mishra,200690225232Lowry, O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall,1951193265275Marinova, D., F. Ribarova and M. Atanassova,200540255260McCready, R.M., J. Guggolz, V. Silviera and H.S. Owens,19502911561158McDonald, S., P.D. Prenzler, M. Antolovich and K. Robards,2001737384Nostro, A., M.P. Germano, V. D'Angelo, A. Marino and M.A. Cannatelli,200030379384Pullaiah, T.,2006Vol.1,pp: 223pp: 223Arshad, J.,2010Parthenium hysterophorus: A review.]]>25331344Scandalios, J.G.,197425225258Schroeder, G., M. Rohmer, J.P. Beck, and R. Anton,1980Euphorbia fischerriana.]]>1922132215Singleton, V.L. and J.A. Rossi,196516144158Verma, C., S. Bhatia and S. Srivastava,20109779785Zlatkis, A., B. Zak and A.J. Boyle,195341486492Zhishen, J., T. Mengcheng and W. Jianming,199964555559Jothi, G.J., A. Benniamin and V.S. Manickam,200812118126Karim, A., M.N. Sohail, S. Munir and S. Sattar,20117419439Sohail, M.N., A. Karim, M. Sarwar and A.M. Alhasin,2011Allium cepa L.): An alternate medicine for Pakistani population.]]>7736744Sohail, M.N., F. Rasul, A. Karim, U. Kanwal and I.H. Attitalla,2011611251152Sohail, F. and M.N. Sohail,2011Silybum marianum L. despite of its weedy nature is an important medicinal plant of NWFP, Pakistan.]]>7747748