Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2010.209.215 Thermomyces lanuginosus TISTR 3465]]>KhucharoenphaisanK. TokuyamaS. RatanakhanokchaiK. KitpreechavanichV. 52010135The effect of carbon sources on the production of β-xylanase by Thermomyces lanuginosus TISTR 3465 was investigated. Xylan showed the highest inductive effect on the enzyme formation whereas xylobiose and xylooligosaccharides resulted in lesser effect. β-Xylanase was also produced at low level with xylose as well as other sugars tested. Xylan concentration at 15 g L-1 gave the maximum inductive effect on β-xylanase formation, whereas xylooligosaccharides and xylose were effective at a lower concentration of 2.5 g L-1. High concentrations of these sugars significantly repressed the enzyme formation. Crude enzyme from the supernatants, without and with other sugars produced a single xylanase band on non-denaturing PAGE gels. However, an intense xylanase activity band was observed from the supernatant of media with xylan, xylobiose and xylooligosaccharides as the carbon sources. An intense protein band of 24.9 kDa from the culture filtrate of xylan medium was observed. Xylan increased β-xylanase production by the fungus 16-fold when it was added to the xylose medium after cultivation for 3 days. In contrast, addition of xylose to the xylan medium decreased β-xylanase production 3-fold. A distinct appearance and disappearance of a 24.9 kDa protein and the activity band coincided with an increase and decrease of xylanase activity, respectively. This indicated the synthesis of xylanase by this strain was most induced by xylan. Moreover, the level of xylanase induction has no related to amino acid sequence of the enzyme.]]>Abdeshahian, P., N. Samat and W.M. Wan Yusoff,2009Aspergillus niger FTCC 5003 using palm kernel cake in fermentative bioprocess.]]>1210491055Ahmed, S., Qurrat-ul-Ain, A. Nighat, N. Saima, Sajjad-ur-Rahman and J. Amer,2003Trichoderma harzianum with different carbon sources.]]>619121916Anand, L., S. Krishnamurthy and P.J. Vithayathil,1990Humicola lanuginosa (Griffon and Maublanc) Bunce.]]>276546553Beg, Q.K., M. Kapoor, L. Mahajan and G.S. Hoondal,200156326338Domsch, K.H., W. Gams and T.H. Anderson,1993Felsenstein, J.,198539783791Ghosh, M. and G. Nanda,1994Aspergillus sydowii MG49.]]>117151156Hidalgo-Lara, M.E. and G.S.A. Farres,2005Cellulomonas flavigena.]]>32345348Hrmova, M., E. Petrakova and P. Biely,1991Aspergillus terreus by homo and heterodisaccharides composed of glucose and xylose.]]>137541547Jäger, A., M. Sinner, H. Purkarthofer, H. Esterbauer and M.G. Ditzel,19921992Khucharoenphaisan, K., S. Tokuyama, K. Ratanakhanokchai and V. Kitpreechavanich,2009Thermomyces lanuginosus strains on thermostable xylanase production.]]>816081614Khucharoenphaisan, K., S. Tokuyama and V. Kitpreechavanich,2008Thermomyces lanuginosus.]]>34187192Kitpreechavanich, V., M. Hayashi and S. Nagai,1984Aspergillus fumigatus and Humicula lanuginosa.]]>626369Kyu, K.L., K. Ratanakhanokchai, D. Uitapap and M. Tanticharoen,1994Bacillus circulans B6.]]>48163167Laemmli, U.K.,1970227680685Lin, J., L.M. Ndlovu, S. Singh and B. Pillay,1999Thermomyces lanuginosus-SSBP.]]>307379Lowry, O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall,1951193265275Miller, G.L.,195931426428Mishra, R.S. and R. Maheshwari,1996Thermomyces lanuginosus: Their purification, properties, action on starch and response to heat.]]>21653672Purkarthofer, H. and W. Steiner,1995Thermomyces lanuginosus.]]>17114118Rapp, P. and F. Wangner,1986Cellulomonas uda.]]>51746752Singh, S., A.M. Madlala and B.A. Prior,2003Thermomyces lanuginosus: Properties of strains and their hemicellulases.]]>765114Singh, S., B. Pillay and B.A. Prior,2000Thermomyces lanuginosus strain.]]>26502508Sunna, A. and G. Antranikian,1997173967Viikari, L., M. Tenkanen, J. Buchert and M. Ratto,19931993Yasui, T., B.T. Nguyen and K. Nakanishi,1984Cryptococcus flavus.]]>62353359