Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2009.914.918hbs Gene Detection]]>GhadamP. GharaviS. YarianF. SoudiM.R. KazemiB. BandehpourM. 1220091212Among some Bacillus species, a protein highly homologous to HU, classified HB and coded by hbs gene. According to the recent studies, the sequence of hbs gene just in one strain of Bacillus subtilis exists in gene bank (ATCC 23857). In this study, DNA from Bacillus subtilis ATCC 6633 was extracted and investigated by PCR. The PCR product was sequenced and shown to differ in just one nucleotide from B. subtilis ATCC 23857. Hence, it was chosen as reference and for the first time, used for non-radioactive labeled probe preparation. The PCR product in Bacillus subtilis with ATCC 6633 was labeled using non-radioactive DIG-labeled nucleotides and conditions of probe preparation and hybridization were optimized and checked it by Southern blotting.]]>Dixon-Fly, S.M. and L. Caro,1999in vitro and in vivo of new HU family protean from Streptococcus thermopiles ST11.]]>42159173Kamaua, E., N.D. Tsihlis, L.A. Simmons and A. Grove, 20053904955Kohler, P. and M.A. Marahiel,1997Bacillus subtitles.]]>17920602064Kunst, F., N. Ogasawara, I. Moszer, A.M. Albertini and G. Alloni et al., 1997Bacillus subtilis.]]>390249256Micka, B., N. Groch, U. Heinemann and M.A. Marahiel, 1991Bacillus subtilis gene encoding the DNA-binding protein HBsu.]]>17331913198Micka, B. and M.A. Marahiel,1992Bacillus subtitles.]]>74641650Oberto, J., K. Drlica and J. Rouviere-Yaniv, 199476901908Ross, M.A. and P. Setlow2000Bacillus subtilis HBsu protein modifies the effects of alpha/beta-type, small acid-soluble spore proteins on DNA.]]>18219421948Schmid, M.B., 199063451453Swinger, K.K. and P.A. Rice,200736510051016Ussery, D., T. Schou Larsen, K.T. Wilke, C. Friis, P. Worning, A. Krogh and S. Brunak, 2001Escherichia coli.]]>83201212Wolfgang, K. and M.A. Marahiel, 2002Bacillus subtilis DNA-binding proteins, HBsu and AbrB.]]>4323329Yokohama, E., K. Doi and S. Ogata, 1997hup gene encoding the histone-like protein HSl of Streptomyces lividans.]]>1353103106Yamane, K., K. Bunai and H. Kakeshita,2004Bacillus subtilis]]>6820072023