Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2009.750.757SohrabiM. KalatiF.A. VatanseverS. AbbasiM.M. RoshangarL. KhakiA.A. TugluI.M. AydemirI. DustarY. JavadzadehY. RadJ. Soleimani 1020091210The aim of the study is to determine the effect of Celecoxib administration, dietary or topical, on expression of Ki-67, c-erb-B2, bcl-2 and bax genes in rat tongue by the immunohistochemistry methods and also tdt-mediated dupt-biotin nick end labeling assay in order to explore their role in malignant transformation and the proliferation rate, apoptosis rate in tongue squamous cell carcinoma. Effects of celecoxib on tongue carcinogenesis were investigated in 40 adult male Sprague Dawley 3-3.5 months rats initiated with 30 ppm 4-nitroquinoline-1-oxide. The immunohistochemical expression of Ki-67, bcl2, bax and c-erb-B2 were also examined for analysis of the effects of Celecoxib on tongue carcinogenesis. Differences among groups were statistically analyzed with one-way analysis of variance (SPSS-13, p<0.05 ). At week 8, the incidence of tongue precancer lesions was reduced by Celecoxib and there were significant differences in the average expression of Ki-67 (p = 0.00), c-erb-B2 (p = 0.01), bax (p = 0.02), bcl2 (p = 0.02) and also in TUNEL assay (p = 0.00). The results suggest probably thatthe level of c-erb-B2, bcl-2 and bax expression could show behavior of squamous cell carcinoma in initiation phase of developing carcinoma.]]>Ananthanarayanan, V., R.J. Deaton, X.J. Yang, M.R. Pins and P.H. Gann,20046119Atula, S., R. Grenman, P. Laippala and S. Syrjänen,199612213131319Chan, T.A., P.J. Morin, B. Vogelstein and K.W. Kinzler,199895681686Gimenez-Conti, I.B. and I.T. Slaga,1993178390Hsu, A.L., T.T. Ching, D.S. Wang, X. Song, V.M. Rangnekar and C.S. Chen,20002751139711403Jaeckel, E.C., S. Raja, J. Tan, S.K. Das, S.K. Dey, D.A. Girod, T.T. Tsue and T.R. Sanford,200112712531259Jendrossek, V., R. Handrick and C. Belk,20031715471549Mann, J.R., M.G. Backlund and R.N. DuBois,20052202210Khawaja, T., S.H. Khan and N.N. Ansari,20076860865Pindborg, J.J., P.A. Reichart, C.J. Smith and I. van der Waal,19972nd Edn.,pp: 21-31pp: 21-31Resnicoff, M., J.L. Burgaud, H.L. Rotman, D. Abraham and R. Baserga,19955537393741Shirasun, K., Y. Hayashido, M. Sugiyama, H. Yoshioka and T. Matsuya,199141349353Slaughter, D.P., H.W. Southwick and W. Smejkal,19536963968Sudbo, J., A. Ristimäki, J.E. Sondresen, W. Kildal, M. Boysen and H.S. Koppang et al.,200339497505Tomei, L.D. and F.O. Cope,19941 Edn.,Wang, Z., C.F. Fuentes and S.M. Shapshay,2002112839843Xie, X., P. De Angelis, O.P. Clausen and M. Boysen,199935502509Yang, E. and S.I. Korsmeyer,199688386401Yao, L., M. Iwai and I. Furuta,1999355662Odukoya, O. and G. Shklar,198254547552