Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2009.530.533Toxoplasma Infected Small Mice to Detect IgG Against Toxoplasma]]>AbdollahiSeyed Hossein ArababadiMohammad Kazemi HassanshahiGholamhossein 62009126The present study, evaluated, using the components of peritoneal fluid of infected mice (as another source of E/SA), for the detection of Toxoplasma specific IgG in human serum. Peritoneal fluids of mice infected by intraperitoneal (IP) inoculation of Toxoplasma tachizoites were collected after 3 days and centrifuged at 750 x g for 15 min then the supernatant was precipitated with ammonium sulphate solution (40% saturated) and used as components containing E/SA. Forty nine uninfected ( without anti-Toxoplasma antibodies) and thirty two positive (with IgG to Toxoplasma) human serum samples were selected (all sera were first tested by standard method for detection of IgG antibodies anti-T.gondii) then the sera were tested by ELISA using E/SA. The cut-off point with 95% confidences was found to be 0.78. Moreover, sensitivity and specificity of the method were determined to be 84 and 92%, respectively. The present results indicate that peritoneal exudates from mice infected with T.gondii may be used as a source of antigenic material for the detection of Toxoplasma-specific IgG and may be valuable for the development of new tools in the serodiagnosis of toxoplasmosis.]]>John, D.T. and W.A. Petri,20069th Edn.,pp: 140-149pp: 140-149Aubert, D., G.T. Maine, I. Villena, J.C. Hunt and L. Howard et al.,2000Toxoplasma gondii-specific immunoglobulin G and immunoglobulin M in human sera by enzyme immunoassay.]]>3811441150Pitetkiewicz, H., E. Hiszcznska-Sawicka, J. Kur, E. Petersen and H.V. Nielsen et al.,2004Toxoplasma gondii-specific recombinant antigens in serodiagnosis of human toxoplasmosis.]]>4217791781Daryani, A., A.Z. Hosseini and A. Dalimi,2003Toxoplasma gondii tachyzoites in the murine model.]]>113123134Eui, S.S. and W.N. Ho,2001Toxoplasma gondii by monoclonal antibodies.]]>394956Ahn, M.H. H.J. Son, M.H. Leem and D.Y. Min,1994Toxoplasma gondii lysate and excretory-secretory materials by enzyme-linked immunoelectrotransfer blot (EITB).]]>32249257Gulie, D.J. and E.H. Richard,19961996pp: 33-59pp: 33-59Yamamoto, Y.I., J.R. Mineo, C.S. Meneghisse, A.C.S. Guimaraes and M. Kawarabayashi,1998Toxoplasma gondii by use immunoblot assay of IgG, IgM and IgA from of dot ELISA.]]>922330Tanyuksel, M., C. Guney, E. Araz, M.A. Saracli and L. Doganci,200442211215Laurence, L., P.F. Marie, M. Corinne, S. Eric, E. Masy and F.C. Marie,2000Toxoplasma gondii for detection of immunoglobulin G antibodies.]]>7607611Alessader, C.A.R., A.S. Maria and R.M. Jose,2002Toxoplasma gondii in samples human serum.]]>9710091013Costa-Silva, T.A., C.S. Meira, I.M. Ferreira, R.M. Hiramoto and V.L. Pereira-Chioccola,2008Toxoplasma gondii.]]>120227234Patricia, R.B.A. and W.F. Antonio,2008Toxoplasma gondii: Immunological marker for acute recent toxoplasmosis.]]>41142147Hafid, J., H. Raberin, B. Pozzetto, Z.Y. Akono, R.T. Sung. M. Jana and O. Gaudin,1992Toxoplasma gondii by enzyme immunoassay and immunoblotting.]]>2210831088