Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2009.1253.1259Rhodobacter sphaeroides NCIMB 8253]]>Syed JaaparS. Za`imahKalilM. S.AnuarN. 1220091218Photo fermentation is a biological process that can
be applied for hydrogen production. The process is environmental friendly
which is operated under mild conditions using renewable resources. In
order to increase yield of H2 produced by Rhodobacter sphaeroides,
some experimental factors that may enhance H2 production were
studied. The effect of operating parameters including agitation, aeration
and light on hydrogen production using R. sphaeroides NCIMB 8253
was investigated. Rhodobacter sphaeroides NCIMB 8253 was grown
in 100 mL serum bottle containing growth medium with maliec acid as the
sole organic carbon source. The cultures were incubated anaerobically
at 30°C with tungsten lamp (100 W) as the light source (3.8 klux)
and argon gas was purged for maintaining anaerobic condition. The results
show that maximum hydrogen produced was higher (54.37 mL) in static culture
with 69.98% of H2 in the total gas compared with shake culture
(11.57 mL) with 57.86% of H2. By using static culture, H2
produced was five times higher compared with non-static in both aerobic
and anaerobic condition. It was found that growth and H2 production
with fluorescent lamp showed better results than growth and H2
production with tungsten light.]]>Akkerman, I., M. Janssen, J. Rocha and H.R. Wijffels,20022711951208Barbosa, M.J., J.M.S. Rocha, J. Tramper and R.H. Wijffels,2001852533Basak, N. and D. Das,2007233142Das, D. and T.N. Veziroglu,2001261328Dogrusoz, N., U. Gunduyz and M. Yucel,2004Rhodobacter sphaeroides O.U.001.]]>2004Eroglu, I., K. Aslan, U. Gunduz, M. Yucel and L. Turker,1999Rhodobacter sphaeroides in a column photobioreactor.]]>70103113Fang, H.H.P., H. Zhu and T. Zhang,2006Clostridium butyricum and rhodobacter sphaeroides. ]]>3122232230Fascetti, E., E. D'Addario, O. Todini and A. Robertiello,199823753760Gadhamshetty, V., A. Sukumaran, N. Nirmalakhandan and M.T. Myint,20083321382146Hoekema, S., B. Martijn, M. Janssen, J. Tramper and H.W. Rene,20022713311338Kalil, M.S., Y. Pang, Y. Sadazo, A.R. Rakmi and M. Wan, 2003612731275Kapdan, K.I., F. Kargi, R. Oztekin and H. Argun,2009Rhodobacter sp.]]>3422012207Kemavongse, K. and P. Prasertsan,2008Rhodobacter sphaeroides U7.]]>2420732085Kim, J.S., H. Yamauchi, K. Ito and H. Takahashi,19824614691474Koku, H., I. Eroglu, U. Gunduz, M. Yucel and L. Turker,2002Rhodobacter sphaeroides. ]]>2713151329Koku, H., I. Eroglu, U. Gunduz, M. Yucel and L. Turker,2003Rhodobacter sphaeroides O.U. 001.]]>28381388Levin, D., L. Pitt and M. Love,200429173185Matsunaga, T., T. Hatano, A. Yamada and M. Matsumoto,200068647651Nakada, E., S. Nishikata, Y.Asada and J. Miyake,19992410531057Uyar, B., I. Eroglu, M. Yucel, U. Gunduz and L. Turker,20073246704677Pang, W.K.,2005Clostridium acetobutylicum NCIMB13357.]]>2005Zhu, H., T. Wakayama, Y. Asada and J. Miyake,20014+.]]>2611491154Kapdan, I.K. and F. Kargi,200638569582