Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2009.1102.1110ThobunluepopPitipong 1220091216 Presently, chemical seed treatments are in discussion due to their directly or indirectly impacts on human health or other living organisms. They may also negatively affect the ecosystem and the food chain. In rice seeds, chemicals may cause phytotoxic effects including seed degradation. Eugenol is the main component of clove (Eugenia caryophillis) oil, which was proved to act simultaneously as bactericide, virocide and especially fungicide. The in vitro study was aimed to compare the inhibitory effect of the following seed treatment substances against seed borne fungi and their shelf-life during 12 months of storage; conventional captan (CA), chitosan-lignosulphonate polymer (CL), eugenol incorporated into chitosan-lignosulphonate polymer (E+CL) and control (CO). The obtained results of fungi inhibition were classified in three groups, which showed at first that CA treatment led to a better, i.e., longer, inhibitory effect on Alternaria padwickii, Rhizoctonia solani, Curvularia sp., Aspergillus flavus and Aspergillus niger than E+CL. Secondly, E+CL coating polymer showed the longest inhibitory effect against Bipolaris oryzae and Nigrospora oryzae compared to CA and CL coating polymer. Finally, both CA and E+CL coating polymer had non-significant difference inhibitory effect on Fusarium moniliforme. The variant of CL coating polymer for seed coating was only during the first 6 months of storage able to inhibit all species of the observed seed borne fungi, whereas CA and E+CL coating polymer were capable to inhibit most of the fungi until 9 months of storage.]]>Baruah, P., R.K. Sharma, R.S. Singh and A.C. Ghosh,1996Fusarium moniliforme.]]>8411412Burt, S.A. and R.D. Reinders,2003Escherichia coli 0157:H7.]]>36162167Chami, N., F. Chami, S. Bennis, J. Trouillas and A. Remmal,20048217226Chami, N., F. Chami, S. Bennis, T. Bouchikhi and A. Remmal,200519405408Chami, N., S. Bennis, F. Chami, A. Aboussekhra and A. Remmal,2005in vitro and in vivo.]]>20106111Dix, N.J. and J. Webster,1995pp: 549pp: 549Don, T.M., S.C. Hsu and W.Y. Chiu,20013916461655Enokibara, S., A. Suzuki, C. Fujita, M. Kashiwagi, N. Mori and Y. Kitamoto,199334221228Ester, A., H. De Putter and J.G.P.M. Van Bilsen,2003Brassica oleracea L. convar. Capitata L.) and Cualifower Brassica oleracea L. var. Botryti L.) with inidacloprid and spinosad to control insect pests.]]>22761768De Souza, E.L., E. de Oliveira Lima, K.R. de Luna Freire and C.P. de Sousa,200548245250Farag, R.S., Z.Y. Daw and S.H. Abo-Raya,1989Aspergillus parasiticus growth and production of aflatoxins in a synthetic medium.]]>547476Han, J.H.,199613287298Han, J.H. and J. Floros,199981114Han, J.H.,2000545665Hewett, P.D.,1987157377Horita, H. and F. Kodama,1996Fusarium roseum in commercial production of cutting of carnation, Dianthus caryophyllus.]]>11840844International Seed Testing Association (ISTA),20062006Jun, Q., R. Wang, J. Yan and J. Hu,2005477581Lee, C.F., C.F. Wen, C.L. Lin and W.Y. Chiu,2004N-isopropylamide-chitosan) copolymers and their application to drug release.]]>4230293037Mungkornasawakul, P., S.G. Pyne, A. Jatisatienr, D. Supyen and W. Lie et al.,200366980982Nah, J.W. and M.K. Jang,20024037963803Ngah, W.S. and K.H. Liang,19993814111414Nugraha, E.S., C. Alain, T. Lin and C. Veronique,2004Lactic acid) blends.]]>1216Ouattara, B., S. Simard, G.J.P. Piette, R.A. Holley and A. Begin,199932428Padgett, T., I.Y. Han and P.L. Dawson,19986113301335Paster, N., M. Menasherov, U. Ravid and B.J. Juven,1995588185Pawar, V.C. and V.S. Thaker,2006In vitro efficacy of 75 essential oils against Aspergillus niger.]]>49316323Smith, J.P., H.S. Ramaswamy and B.K. Simpson,1990107686Tullio V., A. Nostro, N. Mandras, P. Dugo and G. Banche et al.,200610215441550Velluti, A., V. Sanchis, J.A. Ramos and S. Marin,20041 production by Fusarium verticillioides in maize.]]>8411411146Wang, S., L. Chen and Y. Tong,2006446868Zambonelli, A., A.Z. D'Aulerio, A. Bianchi and A. Albasini,1996in vitro.]]>144491494Roller, S. and N. Covill,1999476777Labuza, T.P. and W.M. Breene,198913169Ultee, A., M.H.J. Bennik and R. Moezelaar,2002Bacillus cereus.]]>6815611568