Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2008.876.881MirlohiM. Soleimanian-ZadS. Sheikh-ZeiondinM. FazeliHossein 62008116Regarding the importance of the presence of intestinal
lactobacilli and their population in infants, four different treatments
were evaluated for Lactobacillus isolation efficiency via reduction
in the growth of other groups of bacteria capable of growing on de-Man
Rogosa Sharpe (MRS) medium from fecal samples of 11 Iranian infants. MRS-Vancomycin
(1 mg L-1) was used as a base medium and application of lactic
acid and aerobic incubation of inoculated plates were performed as selective
factors. Each fecal sample was cultivated as duplicate on to the base
medium with or without lactic acid to reduce the pH to 5.4 ±0.2.
Half of the plates were incubated aerobically and the rest of them incubated
under 10% CO2 concentration. Total count and Lactobacillus
count of all samples were recorded according to the age differences of
infants. The counts of false positive colonies were recorded with respect
to their cell morphology and gram reaction in all treatments. Anaerobic
incubation of lactic acid modified MRS-Vancomycin gave the most Lactobacillus
percentage coverage, about 93% among the Lactobacillus positive
samples. Using this treatment, the median Lactobacillus count yielded
8.29 log10 cfu g-1 in the younger and 5.70 log10
cfu g-1 in the elder group. It could be concluded that lactic
acid might be a proper pH reducing agent when enumeration of lactobacilli
from fecal samples is of interest.]]>Ahrné, S., E. Lönnermark, A.E. Wold, N. Aberg and B. Hesselmar et al.,2005712561262Beasley, S.H.,20042004Björkstén, B., E. Sepp, K. Julge, T. Voor and M. Mikelsaar,2001108516520Halm, M., T. Hornbaek, N. Arneborg, S. Sefa-Dedeh and L. Jespersen,20049497103Hartemink, R., V.R. Domenech and F.M. Rombouts,1997297784Hartemink, R. and F.M. Rombouts,199936181192Jackson, M.S., A.R. Bird and A.L. McOrist,2002Lactobacilli in human faeces.]]>51313321Kandler, O. and N. Weiss,19861986pp: 1216pp: 1216Leuschner, R.G.K., J. Bew, V. Coeuret, J.P. Vernoux and M. Gueguen,2003205766Marzotto, M., C. Maffeis and T.H. Paternoster,2006157857866Mikelsaar, M., R. Mändar, E. Sepp and H. Annuk,20042004pp: 455pp: 455Mitsuoka, T.,19921992pp: 69-98pp: 69-98Mountzouris, K.C., A.L. McCartney and R.G. Gibson,200287405420Phillips, M., K. Kailasapathy and L. Tran,2006L. acidophilus, Bifidobacterium sp., L. casei, L. paracasei and L. rhamnosus) in cheddar cheese.]]>108276280Pieterse, B., R.J. Leer, H.J. Schuren and M.J. van derWerf,200515138813894Rogosa, M., J.A. Mitchell and R.F. Wiseman,195162132133Sabine, D.B. and J. Vaselekos,1965206960961Scardovi, V.,1986Bifidobacterium.]]>1986pp: 1418-1435pp: 1418-1435Sharpe, M.E.,19861986pp: 1653-1662pp: 1653-1662Siegumfeldt, H., K.B. Rechinger and M. Jackobsen,20006623302335Thamaraj, N. and N.P. Shah,2003Lactobacillus delbrueckii ssp. bulgaricus, Streptococcus thermophilus, Lactobacillus acidophilus, bifidobacteria, Lactobacillus casei, Lactobacillus rhamnosus and propionibacteria.]]>8622882296Vael, C., A. De Coninck, C. Lammens, K. Desager and H. Goossens,20052005Westerbeek, E.A.M., A. Van den Berg, H.N. Lafeber, J. Knol, W.P.F. Fetter and R.M. Van Elburg,200625361368