Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2008.726.732In vitro Regeneration of Irvingia gabonensis by Somatic
Embryogenesis]]>Fotso Oumar NicolasNiemenak Tchinda NehemieDonfagsiteli Ndoumou DenisOmokolo 52008115 A productive genotype of Irvingia gabonensis were
cultured in vitro for induction embryogenic calli, somatic embryogenesis
and regeneration of plantlets. Fragments of young leaves were used as
primary explants. Callogenesis was initiated by culture of explants during
30 days on Murashige and Skoog medium half strength (MS/2) supplemented
with 1-6 mg L-1 of 2,4-dichlorophenoxyacetic acid (2,4-D).
The highest percentage of explants forming calli is 85.1% at 3 mg L-1
of 2,4-D. Somatic embryos were obtained after a sub-culture of embryogenic
calli during 60 days on MS/2 supplemented with 1-3 mg L-1 of
BAP. The highest percentage of embryogenic calli which differentiates
somatic embryos is 63.8 ± 2.3% at 1 mg L-1 of 6-benzylaminopurine
(BAP). The highest number of somatic embryos per callus which is 43.6
is obtained with 2 mg L-1 of this phytohormone. When isolated
from calli and sub-cultured during 30 days on MS/2 supplemented with 2
mg L-1 of BAP, somatic embryos germinate with a highest percentage
of 83%. The sub-culture of germinated somatic embryos on the same Basal
Medium (BM) supplemented with 4 mg L-1 of BAP and 2 mg L-1
of Naphthalene Acetic Acid (NAA) during 80 days gives rise to the plantlets
with 82.7 ±4.8% of success. With this combination, each plantlet
has average length of 5.6 cm, bears 3.3 leaves and 7.2 roots with 1 or
2 pivoting roots. Plantlets acclimatized on a mixture sterilized soil/vermiculite
at equal volume survive at 93%. Results of this study constitute a new
way for a production of Irvingia gabonensis seedlings with pivoting
root and they permit to arrest the difficulties of natural and horticultural
reproduction.]]>Akubor, P.I., 199649213219Alemano, L., M. Berthouly and N. Michaux-Ferrière, 1997Theobroma cacao L. zygotic embryogenesis and somatic embryogenesis from floral explants.]]>33163172Alemanno, L., M. Berthouly and N. Michaux-Ferriere,199646187194Blanc, G., L. Lardet, A. Matin, J. Jacob and M.P. Carron, 2002Hevea brasiliensis (Müll Arg.). ]]>5314531462Das, G. and G.R. Rout,2002Plumbago species. ]]>68311314Dhar, U. and J. Upreti, 1999In vitro regeneration of a mature leguminous liana (Bauhina vahlii Wight and Arnott).]]>18664669El Maâtaoui, M., H. Espagna and N. Michaux-Ferrière, 1990Quercus suber) cultured in vitro.]]>66183190Fotso, T.N., Donfagsiteli, D. Mbouna and N.D. Omokolo, 2007In vitro Regeneration of Ricinodendron heudelotii. ]]>163136Gill, M.I.S., Z. Singh, B.S. Dhillon and S.S. Gosal, 1995Citrus reticulate Blanco).]]>63167174Hamzah, A., 1992Hopea odorata stem cuttings.]]>3384385Hatanaka, T., O. Arakawa, T. Yasud, N. Uchida and T. Yamaguchi, 1991Coffea canephora.]]>10179182Huang, T., S. Peng, G. Dong, L. Zhang and G. Li, 2002Citrus grandis (Pummelo): Effects of auxins in callus induction medium.]]>69141146Imani, J., T.L. Thi, G. Langen, B. Arnold-Schmitt and S. Roy et al.,2001Daucus sp. ]]>20537541Jayanthi, M. and P.K. Mandala, 2001Tilophora indica (Burm F Merrill). ]]>37576580Kielly, G.A. and S.R. Bowley, 199235474479Lapido, D.O., J.M. Fondoun and N. Ganga, 1996Irvingia sp.): Some Exploitable Intraspecific Variations.]]>1996pp: 193-205pp: 193-205Litz, R.E., R.C. Hendrix, P.A. Moon and V.M. Chavez, 1998531318Lowe, A.J., A.C.M. Gilles, J. Wilson and I.K. Dowson, 20009831841Luo, Y. and H.U. Koop, 1997Arabidopsis thaliana ecotypes.]]>202387396Mohammed, A.M.A., R. Bala and K. Karen, 2002Limonium bellidifolium, Plumbaginaceae.]]>68127135Morel, G. and R.H Wetmore, 195138141143Murashige, T. and F. Skoog,196215473497Newton, A.C., J.F. Mesen, M.P. Dick and R.R.B. Leakey, 19921992Ngoye, A., 1998Irvingia gabonensis).]]>1998Ndoumou, D.O., G.T. Ndzomo and N. Niemenak,1997Theobroma cacao L.]]>39337347Omokolo, N.D., Fotso, Oumar and D. Mbouna, 2004Irvingia gabonensis by in vitro microcutting. ]]>593138Raji, Y., I.A. Ogunwande, J.M. Adesalo and A.F. Bolarinwo, 2001Irvingia gabonensis in rats.]]>39340345Sane, D., A. Borget, J.L. Verdeil, Y.K. Gassama and Dia, 2000Acacia tortilis subsp. radiata (savi) Brenan.]]>143257266Shiembo, P.N., A.C. Newton and R.R.B. Leakey,1996Irvingia gabonensis, a West African fruit tree.]]>87185192Vivien, T. and J.J. Faure, 1996Africa species of cameroon.]]>1996Von Arnold, S., D. Chapham, U. Egerstotter and L.O. Mo, 1996Picea abies.]]>2039West, M.A.L. and J.J. Harada, 1993513611369Youmbi, E. and Benbadis, 2001In vitro regeneration of plants from axillaries buds and apices of sexual plantlets of Dacryodes edulis (Dom) Lam.]]>56333343Li, Z., A. Traore, S. Maximova and M.J. Guiltinan,1998Theobroma cacao L.) using Thidiazuron.]]>34293299