Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2008.2573.2578MojtahedinA. TamaddonfardE. ZanbouriA. 1220081122In this study, the effects of mepyramine (H1-receptor
antagonist), famotidine (H2-receptor antagonist), physostigmine
(a cholinesterase inhibitor) and atropine (muscarinic-receptor antagonist)
have investigated on the formalin-induced nociception in rats. The effects
of mepyramine and famotidine have also examined on nociceptive changes
induced by physostigmine and atropine. Nociception was induced by intraplantar
injection of formalin (50 μL, 1%) into the right hind paw and the
time spent licking and biting of the injected paw, was taken as a measure
of pain. Formalin induced a marked biphasic (first phase: 0-5 min and
second phase: 15-45 min) pain response. The used drugs did not change
the first phase of formalin-induced pain. Subcutaneous injection of physostigmine
significantly (p<0.05) suppressed pain. Subcutaneous injection of atropine
alone did not change the intensity of pain, but pretreatment with atropine
significantly (p<0.05) prevented physostigmine-induced antinociception.
Intraperitoneal injections of mepyramine and famotidine significantly
(p<0.05) decreased pain response. Mepyramine did not significantly
change, but famotidine significantly (p<0.05) prevented analgesic effect
of physostigmine on pain. Atropine did not inhibit the antinociceptive
effects of both mepyramine and famotidine on formalin-induced nociception.
These results indicate that physostigmine through muscarinic cholinergic
receptors suppresses the pain induced by formalin. Both H1
and H2 receptor antagonists produce antinociception. Histamine
H2, but no H1 antagonists may be involved in physostigmine-induced antinociception.]]>Abacioglu, N., A. Bediz, L. Cakici, B. Tuncutan and I. Kanzik,19931-and H2-antihistaminics in mice.]]>2411731176Abbott, F.V. and M. Bonder,1997140553557Buerkle, H., M. Boschin, M.A.E. Marcus, G. Brodner, R. Wutsen and H. Van Aken,19988610271032Carlson, B.B., J.T. Trevitt and J.D. Salamone,20001 antagonists on cholinomimetic-induced tremulous jaw movements: Studies of diphenhydramine, doxepin and mepyramine.]]>65683689Cannon, K.F., R. Leurs and L.B. Hough,20073 receptors inhibits formalin-induced inflammation and nociception, respectively.]]>88122129Capone, F. and A.M. Aloisi,200440223229Coruzzi, G., M. Adami, E. Guaita, I.J. De Esch and R. Leurs,20074-receptor antagonists JNJ7777120 and VUF6002 in a rat model of carrageenan-induced acute inflammation.]]>563240244Cummings, J.L.,2000157415Eidi, M., M.R. Zarrindast, A. Eidi, S. Oryan and K. Parivar,20034659196Farzin, D. and F. Nosrati,200717122128File, S.E., P.J. Kenny and S. Cheeta,2000666572Friedman, A., C.J. Behrens and U. Heineman,200748126130Fu, L.W., W. Schunack and J.C. Longhurst,20051 receptors and PKC.]]>93713722Girard, P., Y. Pansart, M.C. Coppe, D. Verniers and J.M. Gillardin,20045036369Hacimuftuoglu, A., C.R. Handy, V.M. Goettl, C.G. Lin, S. Dane and R.L. Jr. Stephens,2006173211216Hasselmo, M.E.,200616710715Jones, P.G. and J. Dunlop,200753197206Koppert, W., P. Martus and P.W. Reeh,2001597106Le Bars, D.L., M. Gozariu and S.W. Cadden,200153597652Liu, H., Q. Zheng and J.M. Farley,200613395401Milenov, K., S. Todorov, M. Vassileva, R. Zamfirova and A. Shahbazian,19951 and H2 blockers on the tone and the contractile activity of guinea pig stomach fundus.]]>17609613Mimaki, H., S. Kawauchi, S. Kagawa, S. Ueki and K. Takeuchi,20012-receptor antagonist, in rat duodenums.]]>95165171Mojtahedin, A., E. Tamaddonfard and A. Zanboori,20082008Mochizuki, T., K. Okakura-Mochizuki, A. Horii, Y. Yamamoto and A. Yamatodani,1994in vivo.]]>6222752282Nonogaki, K., X. Li, T. Tamagawa, G. Watanabe and Y. Hiyoshi et al.,19931- and H2-receptors in neostigmine-induced hyperglycemia in rats.]]>52PL107PL110Olsen, U.B., C.T. Eltrop, B.K. Ingvargsen, T.K. Jorgensen and J.A. Lundbaek et al.,20024354357Oritz, M.I. and G. Castaneda-Hernandez,200812233241Orzechowski, R.F., D.S. Currie and C.A. Valancius,20051 receptor antagonists in two functional models.]]>506257264Patil, C.S. and S.K. Kulkarni,199921523527Poli, E., G. Coruzzi and G. Bertaccini,199030191194Poyhia, R., V.K. Kontinen, S. Paananen and E. Kalso,199989428433Raffa, R.B.,2001268185Raboisson, P. and R. Dallel,200428219226Sakurada, S., T. Orito, C. Sakurada, T. Sato, T. Hayashi and J.I. Mobarakeh et al.,20024342934Tamaddonfard, E. and S. Rahimi,200431518522Tamaddonfard, E., E. Khalilzadeh, N. Hamzeh-Gooshchi and S. Seiednejhad-Yamchi,200860219224Tamaddonfard, E., H. Soraya and N. Hamzeh-Gooshchi,20082008Ting, E., R.C. Roveroni, L.F. Ferrari, C.M. Lotufo and M.C. Veiga et al.,200781765771Watanabe, K., S. Yano, M. Yamamoto and S. Kanaoka,199361229236Wolf, N.G.,200630219222Yang, X.F. Y. Xiao and M.Y. Xu,200811516Yoon, M.H., J.I. Choi and S.W. Jeong,20034710791084Zanboori, A., E. Tamaddonfard and A. Mojtahedin,20081124282432Zhao, B.M., S.M. Moochhala and S.Y. Tham,2004812183192