Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2008.2553.2559Aspergillus oryzae]]>EsfahanibolandbalaieZ. RostamiK. S. MirdamadiS. 1220081122The extracellular α-amylase production by Aspergillusoryzae was studied in submerged fermentation using an Adlof-Kuhner orbital shaker. The effect of initial pH values in the range of 4 to 7.5 on enzyme production was investigated and initial pH medium of 6.2±0.1 resulted in enhanced α-amylase production. The effect of carbon and nitrogen source and composition was examined and it has been observed that corn starch concentration of 15 g L-1 has sound effect on enzyme production. The medium containing corn starch, sodium nitrate resulted in considerable higher enzyme production. Further, the yeast extract of 2.5 g L-1 in the medium produced higher enzyme in view to other organic nitrogen sources. The effect of temperature on α-amylase production from 20 to 40°C has been studied and at 35±1°C higher α-amylase has been obtained. The effect of shaker’s speed on α-amylase production from 50 to 200 rpm was investigated. And at about 180 rpm higher enzyme production has been observed. In the present study, it has been found that glucose has repressing effect on α-amylase production using A. oryzae PTCC5164.]]>Aguilar, G., J. Morlon-Guyot, B. Trejo-Aguilar and J.P. Guyot,2002Lactobacillus manihotivorans LMG 1801 (T), an amylolytique lactic acid bacterium.]]>27406413Bernfeld, P.,19551955pp: 149-158pp: 149-158Bradford, M.M.,197672248254Chiu, L.Y. and W.L. Chandler,199541S238S239Crueger, W. and A. Crueger,19841st Edn.,pp: 189-218pp: 189-218Djekrif-Dakhmouche, S., Z. Gherib-Aoulmi, Z. Meraihi and L. Bennamoun,2006Aspergillus niger ATCC 16404 grown on orange waste powder.]]>73190197Florence, G.B., T.M. Chambers and D.L. Wiedbrank,19921st Edn.,Fogarty, W.W.,19831st Edn.,pp: 1-22pp: 1-22Fogarty, W.M. and C.T. Kelly,19801980pp: 115-170pp: 115-170Kundu, A.K., S. Das and T.K. Gupta,1973Aspergillus oryzae.]]>51142150Lepp, C.A., S. Gilligan, J.J. Tobin, G. Gunzer and P. Conway et al.,1996422323Morkeberg, R., M. Carlsen and J. Nielsen,1995Aspergillus oryzae.]]>14124492454Nahas, E. and M.M. Waldemarin,2002Aspergillus ochraceus.]]>44510Pandey, A., P. Nigam, C.R. Soccol, V.T. Soccol, D. Singh and R. Mohan,200031135152Rosell, C.M., M. Haros, C. Escriva and C.B. de Barber,20014929732977Spohr, A., M. Carlsen, J. Nielsen and J. Villadsen,1998Aspergillus oryzae during fed-batch and continues cultivation.]]>864956Sudo, S., T. Ishikawa, Y. Takayasu-Sakamoto, K. Sato and T. Oba,1993Aspergillus kawachini.]]>76105110Sudo, S., T. Ishikawa, K. Sato and T. Oba,1994Aspergillus kawachini: Solid-state and submerged cultures.]]>77483489Viswanthan, P. and N.R. Surlikar,2001Aspergillus flavus on Amaranthus grains by solid-state fermentation.]]>415764Witezak, Z.,19996165178Egas, M.C., M.S. da Costa, D.A. Cowan and E.M. Pires,1998Thermus filiformis Ork A2: Purification and biochemical characterization.]]>22332