Pakistan Journal of Biological Sciences
1028-8880
1812-5735
Asian Network for Scientific Information
10.3923/pjbs.2008.2433.2437
Bovine leukemia virus p24 Protein in Bacterial
Cell]]>
Momtaz
H.
Hemmatzadeh
F.
Keyvanfar
H.
12
2008
11
20
Bovine leukemia virus (BLV) is a member of the
family Retroviridae, genus Deltaretrovirus that has three important
gene including gag, pol and env. This virus causes B-cell
lymphocytosis and lymphosarcoma in cows. In the first step PCR product
of gag gene of BLV isolated in different regions of Iran and BLV-FLK
strain were cloned in to a pTZ57R/T vector, then insert were digested
by BglII and XhoI restriction enzymes and cloned in to pET-28(a)
as an expression vector. For the expression of p24 protein, the pET-28(a)
recombinant vector was transformed in BL21(DE3) strain of E. coli
competent cell and after induction of the protein having been expressed
by IPTG, the presence of gag expressed protein was shown in immunoblotting
and SDS-PAGE system. With respect to the remarkable frequency of infection
to BLV in Iran and the necessity of controlling it through vaccination
with recombinant vaccines of gp51, manufacturing and applying the recombinant
p24 protein are vital goals in recognition and distinction between infection
and responses caused by vaccine.]]>
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