Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2008.2418.2422KimB. Boon KadirH. Abdul TayyabS. 1220081120Interaction of bromophenol blue (BPB) with serum albumins
from different mammalian species, namely, human (HSA), bovine (BSA), goat
(GSA), sheep (SSA), rabbit (RbSA), porcine (PSA) and dog (DSA) was studied
using absorption and absorption difference spectroscopy. BPB-albumin complexes
showed significant differences in the spectral characteristics, i.e.,
extent of bathochromic shift and hypochromism relative to the spectral
features of free BPB. Absorption difference spectra of these complexes
also showed variations in the position of maxima and absorption difference
(ΔAbs.) values. Absorption difference spectra of different bilirubin
(BR)-albumin complexes showed a significant blue shift accompanied by
decrease in ΔAbs. values in presence of BPB which were indicative
of the displacement of bound BR from its binding site in BR-albumin complexes.
These changes in the difference spectral characteristics of BR-albumin
complexes were more marked at higher BPB concentration. However, the extent
of these changes was different for different BR-albumin complexes. Taken
together, all these results suggest that BPB partially shares BR binding
site on albumin and different mammalian albumins show differences in the
microenvironment of the BR/BPB binding site.]]>Bertsch, M., A.L. Mayburd and R.J. Kassner,2003313187195Bjerrum, O.J.,1968224148Brodersen, R., B. Friis-Hansen and L. Stern,19836217229Carter, D.C. and J.X. Ho,199445153203Faizul, F.M., H.A. Kadir and S. Tayyab,20089017Frandsen, P.C. and R. Brodersen,1986405559Fung, Y.S., D.X. Sun and C.Y. Yeung,200021403410Gourley, G.R.,199744173229Halim, A.A., H.A. Kadir and S. Tayyab,20081443338Honore, B., R. Brodersen and A. Robertson,19836347355Jacobsen, J. and R.P. Wennberg,197420783789Khan, M.M. and S. Tayyab,20002 groups blocking and chloroform binding.]]>1523147153Khan, M.M. and S. Tayyab,20011545263277Lowry, O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall,1951193265275Ostrow, J.D., P. Mukerjee and C. Tiribelli,19943517151737Peters, T. Jr.,19961st Edn.,Rashid, H., S. Muzammil and S. Tayyab,199844165173Tayyab, S. and M.A. Qasim,1990125558Tayyab, S., N.J. Khan, M.A. Khan and Y. Kumar,200331187193Turner, D.C. and L. Brand,1968733813390Tsutsumi, Y., T. Maruyama, A. Takadate, M. Goto, H. Matsunaga and M. Otagiri,199916916923Wei, Y., K. Li and S. Tong,199643110