Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2008.1916.1922EsfandiariE. ShakibaM. R. MahboobS. A. AlyariH. ShahabivandS. 1220081115An investigation was conducted to study the effect of
water stress on the antioxidant content, protective enzyme activities,
proline content and lipid peroxidation in wheat seedlings. Drought stress
increases the amount of Reactive Oxygen Species (ROS), leading to metabolic
disorders. It is now known that higher levels of activity- protective
mechanisms render the cells more enduring against environmental stress
including drought. Two widely cultivated cultivars of wheat in Iran, Sab.
and N. Sar. were grown up according to the hydroponic method. Having reached
the stage of 4-5 leaves growth; the plants were kept under 4, 8 and 12
bars potential resulting from using Polyethylene Glycol 8000 (PEG 8000).
Hogland solution was used as the control. Then the amount of ascorbate,
glutathione, superoxide dismutase and catalase activity, proline and lipid
Peroxidation was measured in cut samples of the leaves. The result indicated
an increase in the amount of Ascorbate and Glutathione as the stress was
intensified in the case of Sab. Moreover, the reduced form of Ascorbate
(ASC) and Glutathione (GSH) were higher in Sab. at 8 and 12 bars. The
amount of Proline accumulation was considerably higher in Sab. than N.
Sar. SOD activity, on the other hand, diminished at 8 and 12 bar levels.
CAT activity is also regarded as a limiting factor. Lipid peroxidation
was also geared up as the stress was intensified. These limiting factors
rendered N. Sar. cultivar more sensitive to water stress resulting from
PEG8000 compared to Sab.]]>Aebi, H.,1984in vitro.]]>1984pp: 121-126pp: 121-126Asada, K.,2006141391396Ashraf, M. and M.R. Foolad,200759206216Bates, L.S., R.P. Waldren and I.D. Teare,197339205207Beak, K.H. and D.Z. Skinner,200316512211227Bor, M., F. Ozdemir and I. Turkan,2003Beta vulgaris L. and wild beet Beta maritima L.]]>1647784Van Breusegem, F., E. Vranova, J.F. Dat and D. Inze,2001161405414Candan, N. and L. Tarhan,2003Mentha pulegium organs grown in Ca2+, Mg2+, Cu2+, Zn2+ and Mn2+ stress conditions.]]>163769779Esfandiari, E.,20072007Esfandiari, E.O., M.R. Shakiba, S.A. Mahboob, H. Alyari and M. Toorchi,20075149153Esfandiari, E., F. Shekari, F. Shekari and M. Esfandiari,2007354856Fadzilla, N.M., R.P. Finch and R.H. Burdon,199748325331Fridivich, I.,198926477617764De Gara, L., M.C. de Pinto and F. Tommasi,200341863870Granczarska, M.,200543583590Jablonski, P.P. and J.W. Anderson,19826914031407Jiang, C.D., H. Gao, Q. Zou, G. Jiang and L.H. Li,2006558796Jimenez, A., J.A. Hernandez, G. Pastori, L.A. del Rio and F. Sevilla,199811813271335Karabal, E., M. Yucel and H.A. Oktem,2003164925933Kono, Y. and I. Fridovich,19832581364613668Kornyeyev, D., B.A. Logan, R.D. Allen and A.S. Holadag,200316510331041De Lacerda, C.F., J. Cambraia, A. Oliva and H.A. Ruiz,2005546976Law, M.Y., S.A. Charles and B. Halliwell,1983Spinacia oleracea) chloroplasts: The effect of hydrogen peroxide and of Paraquat.]]>210899903Lu, C. and J. Zhang,19995011991206Mansour, M.M.F.,199836767772Martinez, C.A., M.E. Loureiro, M.A. Oliva and M. Maestri,2001Solanum curtilobum and freezing sensitive Solanum tuberosum subjected to oxidative and water stress.]]>160505515McCord, J.M. and I. Fridovich,196924460496055Michel, B.E.,1983726670Milone, M.T., C. Sgherri, H. Clijsters and F. Navari-Izzo,200350265276Mittler, R.,20027405410Mittler, R., S. Vanderauwera, M. Gollery and F. van Breusegem,20049490498Morita, S., M. Tasake, H. Fujisawa, T. Ushimaru and H. Tsuji,199410510151016Noctor, G., A.C.M. Arisi, L. Jouanin and C.H. Foyer,1998118471482Potters, G., L.D. Gara, H. Asada and N. Horemans,200240537548Quiles, M.J. and N.I. Lopez,2004166815823Reddy, A.R., K.V. Chaitanya and M. Vivekanandan,200416111891202Sairam, R.K., K.V. Roa and G.C. Srivastava,200216310371046Sànchez, E., J.M. Ruiz and L. Romero,2002Phaseolus vulgaris L. cv Strike).]]>93225233Scandalios, J.G.,1993101712Sen Gupta, A., R.P. Webb, A.S. Holaday and R.D. Allen,199310310671073Shao, H. B., Z.S. Liang, M.A. Shao and B.C. Wang,200542107113Stewart, R.R.C. and J.D. Bewley,198065245248Taylor, N.L., C. Rudhe, J.M. Hulett, T. Lithgow and E. Glaser et al.,2003547125130Uchida, A., A.T. Jagendorf, T. Hibino, T. Takabe and T. Takabe,2002163515523Vaidyanathan, H., P. Sivakumar, R. Chakrabarty and G. Thomas,2003Oryza sativa L.)-differential response in salt-tolerant and sensitive varieties.]]>16514111418Yamazaki, J., A. Ohashi, Y. Hashimoto, E. Negishi and S. Kumagai et al.,2003Abies mariesii growing at the forest limit on Mt. Norikura in Central Japan.]]>165257264Yoshimura, K., Y. Yabute, T. Ishikawa and S. Shigeoka,2000123223233