Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2008.188.194Brucella abortus and Brucella melitensis]]>AmirmozafariN. GhaziF. MostafazadehA. MostafaieA. RajabniaR. 22008112Heat shock protein (hsp) is highly conserved, that
serves a wide range of function in protein folding and transport. It protect
from various type of stress including heat shocks. However, it is well
known that the virulence of B. melitensis is more than B. abortus,
but there is not any strong evidence to verify it. For this purpose, in
refer to potent antigenicity of hsps in various infectious as well as
some hsp molecules act as potent activator of macrophage (danger signal),
we hypothesized that difference in virulence between B. abortus
and B. melitensis may be originated from difference in pattern
of response to heat shock induced by high degree of fever that usually
present in brucellosis. To this end, five B. abortus and five B.
melitensis strains isolated from cows and human, were subjected to
39, 40 and 42 ° C heat shocks. The bacterial whole cell proteins were
extracted and resolved by SDS-PAGE. Western blotting was used to detect
antibody production against the extracted bacterial proteins especially
hsp60 in both control and patient sera. SDS-PAGE gels revealed protein
bands mainly in the range of 10-100 kDa. The amounts of a 60 kDa protein
band (hsp60) was significantly enhanced following heat shock at 42 ° C
in relation to the unheated cells in both bacterial species. The heat
shock responses in B. abortus and B. melitensis point to
the higher production of a 60 kDa protein (hsp60) in both bacterial species,
especially in B. abortus. It seems that, lower hsp60 production
by B. melitensis would induce a relatively much lower immune response
against the bacterium leading to its greater virulence potentials; the
sera from Brucellosis patients reacted with several of these cell derived
protein bands in western blots, none of which were reactive with sera
from healthy individuals. The western blot protein bands showed striking
differences. This observation points to the immunogenic properties of
hsps, specially the overwhelming response to hsp-60. Therefore, hsp-60
can be a good antigenic candidate for engineering subunit vaccine against
Brucella, as well as for ELISA test development.]]>Abdolalizadeh, J., 2002Brucella abortus (S19) proteins by 2-dimensional electrophoresis and Western blot.]]>71121Oñate, A.A., S. Cespedes, A. Cabrera, R. Rivers and A. Gonzalez et al.,2003Brucella abortus induces protective immunity in BALB/c mice.]]>148574861Baloglu, S., T.E. Toth, G.G. Schurig, N. Sriranganathan and S.M. Boyle,2000Brucella abortus GroEL does not correlate with protection against a B. abortus challenge.]]>76193199Corbel, M.J.,19973213221Gomes, A.P.T., A. Cloeckaert and M.S. Zygmunt, 2000Brucella melitensis.]]>6829542961Jawets, E., J.L. Melnick and E.A. Adelberg, 200323rd Edn.,Cassataro, J., K.A. Pasquevich, S.M. Estein, D.A. Laplagne and A. Zwerdling et al.,2007B. ovis and similar degree of protection against B. melitensis than review. 1 vaccination.]]>2559585967Kaufmann, S.H.E., 199011129136Lin, J., L.G. Adams and T.A. Ficht,1992Brucella abortus and isolation of the genes the GroE heat shock proteins.]]>6024252431Lin, J. and T.A. Ficht, 1995Brucella abortus induced during macrophage infection.]]>6314091414Lindler, LE., T.L. Hadfield, B.D. Tall, N.J. Snellings and F.A. Rubin et al.,1996Brucella melitensis group 3 antigen gene encoding omp28. ]]>6424902499Mostafaie, A., 1999Brucella abortus (S19 strain) and Brucella mellitensis (M16 strain).]]>4817Mostafaie, A., Z.M. Hassan and B. Tabaraie, 2000Brucella LPS. ]]>52126Mostafaie, A., 2005Brucella abortus (S19) identified by two-dimensional gel electrophoresis and immunobloting.]]>301015Naoko, Y. K. Yokota, M. Mizuno, Y. Kawahara and M. Adachi et al., 2000Helicobacter pylori eradication treatment.]]>7574577Wei, C., S. Ulrike, B. Kerstin, B. Volker and K. Hubert, 199916232123219Young, E.J.,199521283289Yilan, Z., O. Norio and R. Yasuko, 1998Ehrlichia sennetsu and differential expression of HSP70 and HSP60 mRNA after temperature upshift.]]>6631063112