Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2008.1718.1722Vibrio holerae in Escherichia coli]]>HaryantiT. MarianaN.S. LatifahS.Y. YusoffK. RahaA.R. 1220081113The ctxB gene, the causative agent of cholera
epidemic was successfully cloned from V. cholerae in E. coli.
The insertion of the gene was confirmed by PCR as well as restriction
digestion analyses. The sequencing results for the gene confirmed that
the insert was in the correct orientation and in-frame with the PBAD
promoter and it showed that the gene was 99% homologous to the published
ctxB sequence. The CTB protein was successfully expressed in
E. coli using the pBAD/His vector system. The expected protein
of ~14 kDa was detected by SDS-PAGE and Western blot. The use of pBAD/His
vector to express the cholera toxin gene in E. coli would facilitate
future study of toxin gene products.]]>Bergerot, I., C. Ploix, J. Petersen, V. Moulin and C. Rask et al.,19979446104614Bergquist, C., E.L. Johansson, T. Lagergard, J. Holmgren and A. Rudin,19976526762684Birnboim, H.C. and J. Doly,1979715131523Boomershine, W.P., M.L.S. Raj, V. Gopalan and M.P. Fostera,2003Escherichia coli under the tight control of the araBAD promoter: Expression of an archaeal homolog of the RNase P Rpp29 protein.]]>28246251Briles, D.E., E. Ades, J.C. Paton, J.S. Sampson, G.M. Carlone, R.C. Huebner, A. Virolainen, E. Swiatlo and S.K. Hollingshead,2000Streptococcus pneumoniae.]]>68796800Clark, M.A., F.R. Hammond, A. Papaioannou, N.J. Hawkins and R.L. Ward,1997Escherichia coli arabinose promoter, PBAD.]]>3217226Areas, A.P., M.L. Oliveira, C.R. Ramos, M.E. Sbrogio-Almeida, I. Raw and P.L. Ho,2002Escherichia coli.]]>25481487Guzman, L.M., D. Belin, M.J. Carson and J. Beckwith,1995BAD promoter.]]>17741214130Hagiwara, Y., K. Komase, Z. Chen, K. Matsuo and Y. Suzuki et al.,19991729182926Johnson, C.M. and R.F. Schleif,1995In vivo induction kinetics of the arabinose promoters in Escherichia coli.]]>17734383442Kozuka, S, Y. Yasuda, M. Isaka, N. Masaki, T. Taniguchi, K. Matano, A. Moriyama, K. Ohkuma, N. Goto, S. Udaka and K. Tochikubo,2000Escherichia coli heat-labile enterotoxin B subunit by using the expression/secretion system of Bacillus brevis and its mucosal immunoadjuvancity.]]>1817301737Laemmli, U.K.,1970227680685Lebens, M, V. Shahabi, M. Backstrom, T. Houze, M. Lindblad and J. Holmgren,19966421442150Lee, K.Y., J.D. Hopkins, T.F. O'Brien and M. Syvanen,1991114551Lewis, G., I.W. Taylor, A.W. Nienow and C.J. Hewitt,2004Escherichia coli batch fermentation processes.]]>31311322L'hoir, C., A. Renard and J.A. Martial,1990Escherichia coli of two mutated genes encoding the cholera toxin B subunit.]]>894752Lobell, R.B. and R.F. Schleif,1990250528532Pascale, J.M., M.M. Shaw, P.J. Durant, A.A. Amador and M.S. Bartlett et al.,1999Pneumocystis carinii lung infection.]]>67805809Rudin, A., G.C. Riise and J. Holmgren,19996728842890Sambrook, J., E.F. Fritsch and T.A. Maniatis,19891st Edn.,Sanchez, J. and J. Holmgren,200517388398Snider, D.P., J.S. Marshall, M.H. Perdue and H. Liang,1994153647657Tamura, S.I., Y. Shoji, K. Hashigucci, C. Aizawa and T. Kurata,19941212381240Tamura, S., Y. Samegai, H. Kurata, T. Nagamine, C. Aizawa and T. Kurata,19886409413Tang-Feldman, Y.J., G. Ackermann, J.P. Henderson, J. Silva Jr. and S.H. Cohen,2002Clostridium difficile toxin B gene (tcdB).]]>16179183Wiedermann, U., B. Jahn-Schmid, M. Lindblad, C. Rask, J. Holmgren, D. Kraft and C. Ebner,19991117171724